{"doi":"10.1128/jvi.76.5.2113-2122.2002","title":"Interaction of Translation Initiation Factor eIF4B with the Poliovirus Internal Ribosome Entry Site","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Poliovirus translation is initiated at the internal ribosome entry site (IRES). Most likely involving the action of standard initiation factors, this highly structured\n            <jats:italic>cis</jats:italic>\n            element in the 5\" noncoding region of the viral RNA guides the ribosome to an internal silent AUG. The actual start codon for viral protein synthesis further downstream is then reached by ribosomal scanning. In this study we show that two of the secondary structure elements of the poliovirus IRES, domain V and, to a minor extent, domain VI, are the determinants for binding of the eukaryotic initiation factor eIF4B. Several mutations in domain V which are known to greatly affect poliovirus growth also seriously impair the binding of eIF4B. The interaction of eIF4B with the IRES is not dependent on the presence of the polypyrimidine tract-binding protein, which also binds to the poliovirus IRES. In contrast to its weak interaction with cellular mRNAs, eIF4B remains tightly associated with the poliovirus IRES during the formation of complete 80S ribosomes. Binding of eIF4B to the IRES is energy dependent, and binding of the small ribosomal subunit to the IRES requires the previous energy-dependent association of initiation factors with the IRES. These results indicate that the interaction of eIF4B with the 3\" region of the poliovirus IRES may be directly involved in translation initiation.\n          </jats:p>","journal":"Journal of Virology","year":2002,"id":21021,"datarank":2.3361706567817304,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"self_citation_contribution":0.5897738449086489,"citation_network_contribution":1.7463968118730815,"self_endowment_contribution":0.5897738449086489,"citer_contribution":1.7463968118730815,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":43,"citers_with_citation_signal":37,"citers_with_endowment":37,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136917,"name":"Lanja Saleh","orcid":null,"position":1,"is_corresponding":false},{"id":136918,"name":"Gergis Bassili","orcid":null,"position":2,"is_corresponding":false},{"id":136919,"name":"Volker H. Sonntag","orcid":null,"position":3,"is_corresponding":false},{"id":136920,"name":"Amandus Zeller","orcid":null,"position":4,"is_corresponding":false},{"id":136921,"name":"Michael Niepmann","orcid":null,"position":5,"is_corresponding":false},{"id":136916,"name":"Kerstin Ochs","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11836388","pmcid":"PMC135939","openalex_id":"https://openalex.org/W2100838622","authors":[],"funders":[],"total_grants":0,"fwci":2.9639,"citation_percentile":0.9202452,"influential_citations":1,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":1},{"year":2014,"count":3},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2025,"count":3}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://europepmc.org/articles/pmc135939?pdf=render","host_type":"GREEN"},{"url":"https://journals.asm.org/doi/pdf/10.1128/jvi.76.5.2113-2122.2002","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.76.5.2113-2122.2002","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11836388","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC135939","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/135939","host_type":"repository"}],"fields_of_study":["Viral Infections and Immunology Research","RNA and protein synthesis mechanisms","RNA Research and Splicing","Medicine","Biology","5' Untranslated Regions","Animals","Binding Sites","Eukaryotic Initiation Factors","Humans","Mice","Mutation","Peptide Initiation Factors","Plasmids","Poliovirus","Polypyrimidine Tract-Binding Protein","Protein Biosynthesis","RNA, Messenger","RNA, Viral","RNA-Binding Proteins","Rabbits","Ribonucleoproteins","Ribosomal Proteins","Ribosomes"],"mesh_terms":["Animals","Binding Sites","Humans","Mutation","Peptide Initiation Factors","Plasmids","Rabbits","Ribonucleoproteins","Ribosomal Proteins","Ribosomes","RNA, Messenger","RNA, Viral","Protein Biosynthesis","RNA-Binding Proteins","Poliovirus","5' Untranslated Regions","Polypyrimidine Tract-Binding Protein","Eukaryotic Initiation Factors","Mice"],"keywords":["Internal ribosome entry site","Eukaryotic initiation factor","Initiation factor","Eukaryotic translation","Polypyrimidine tract-binding protein","Biology","Poliovirus","Ribosome","Translation (biology)","Eukaryotic Small Ribosomal Subunit","Eukaryotic Ribosome","Cell biology","30S","Picornavirus","RNA","Molecular biology","Messenger RNA","Virology","Genetics","Virus","RNA-binding protein","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Affordable and clean energy"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T14:06:30.215539Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}