{"doi":"10.1128/jvi.74.2.965-974.2000","title":"Replication of Herpes Simplex Virus Type 1 within Trigeminal Ganglia Is Required for High Frequency but Not High Viral Genome Copy Number Latency","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The replication properties of a thymidine kinase-negative (TK\n            <jats:sup>−</jats:sup>\n            ) mutant of herpes simplex virus type 1 (HSV-1) were exploited to examine the relative contributions of replication at the body surface and within trigeminal ganglia (TG) on the establishment of latent infections. The replication of a TK\n            <jats:sup>−</jats:sup>\n            mutant, 17/tBTK\n            <jats:sup>−</jats:sup>\n            , was reduced by ∼12-fold on the mouse cornea compared to the rescued isolate 17/tBRTK\n            <jats:sup>+</jats:sup>\n            , and no replication of 17/tBTK\n            <jats:sup>−</jats:sup>\n            in the TG of these mice was detected. About 1.8% of the TG neurons of mice infected with 17/tBTK\n            <jats:sup>−</jats:sup>\n            harbored the latent viral genome compared to 23% of those infected with 17/tBRTK\n            <jats:sup>+</jats:sup>\n            . In addition, the latent sites established by the TK\n            <jats:sup>−</jats:sup>\n            mutant contained fewer copies of the HSV-1 genome (average, 2.3/neuron versus 28/neuron). On the snout, sustained robust replication of 17tBTK\n            <jats:sup>−</jats:sup>\n            in the absence of significant replication within the TG resulted in a modest increase in the number of latent sites. Importantly, these latently infected neurons displayed a wild-type latent-genome copy number profile, with some neurons containing hundreds of copies of the TK\n            <jats:sup>−</jats:sup>\n            mutant genome. As expected, the replication of the TK\n            <jats:sup>−</jats:sup>\n            mutant appeared to be blocked prior to DNA replication in most ganglionic neurons in that (i) virus replication was severely restricted in ganglia, (ii) the number of neurons expressing HSV proteins was reduced 30-fold compared to the rescued isolate, (iii) cell-to-cell spread of virus was not detected within ganglia, and (iv) the proportion of infected neurons expressing late proteins was reduced by 89% compared to the rescued strain. These results demonstrate that the viral TK gene is required for the efficient establishment of latency. This requirement appears to be primarily for efficient replication within the ganglion, which leads to a sixfold increase in the number of latent sites established. Further, latent sites with high genome copy number can be established in the absence of significant virus genome replication in neurons. This suggests that neurons can be infected by many HSV virions and still enter the latent state.\n          </jats:p>","journal":"Journal of Virology","year":2000,"id":595387,"datarank":0.6610078870896381,"base_score":4.406719247264253,"endowment":4.406719247264253,"self_citation_contribution":0.6610078870896381,"citation_network_contribution":0.0,"self_endowment_contribution":0.6610078870896381,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1524587,"name":"N. M. Sawtell","orcid":null,"position":1,"is_corresponding":false},{"id":1524586,"name":"Richard L. Thompson","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Replication of Herpes Simplex Virus Type 1 within Trigeminal Ganglia Is Required for High Frequency but Not High Viral Genome Copy Number Latency","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The replication properties of a thymidine kinase-negative (TK\n            <jats:sup>−</jats:sup>\n            ) mutant of herpes simplex virus type 1 (HSV-1) were exploited to examine the relative contributions of replication at the body surface and within trigeminal ganglia (TG) on the establishment of latent infections. The replication of a TK\n            <jats:sup>−</jats:sup>\n            mutant, 17/tBTK\n            <jats:sup>−</jats:sup>\n            , was reduced by ∼12-fold on the mouse cornea compared to the rescued isolate 17/tBRTK\n            <jats:sup>+</jats:sup>\n            , and no replication of 17/tBTK\n            <jats:sup>−</jats:sup>\n            in the TG of these mice was detected. About 1.8% of the TG neurons of mice infected with 17/tBTK\n            <jats:sup>−</jats:sup>\n            harbored the latent viral genome compared to 23% of those infected with 17/tBRTK\n            <jats:sup>+</jats:sup>\n            . In addition, the latent sites established by the TK\n            <jats:sup>−</jats:sup>\n            mutant contained fewer copies of the HSV-1 genome (average, 2.3/neuron versus 28/neuron). On the snout, sustained robust replication of 17tBTK\n            <jats:sup>−</jats:sup>\n            in the absence of significant replication within the TG resulted in a modest increase in the number of latent sites. Importantly, these latently infected neurons displayed a wild-type latent-genome copy number profile, with some neurons containing hundreds of copies of the TK\n            <jats:sup>−</jats:sup>\n            mutant genome. As expected, the replication of the TK\n            <jats:sup>−</jats:sup>\n            mutant appeared to be blocked prior to DNA replication in most ganglionic neurons in that (i) virus replication was severely restricted in ganglia, (ii) the number of neurons expressing HSV proteins was reduced 30-fold compared to the rescued isolate, (iii) cell-to-cell spread of virus was not detected within ganglia, and (iv) the proportion of infected neurons expressing late proteins was reduced by 89% compared to the rescued strain. These results demonstrate that the viral TK gene is required for the efficient establishment of latency. This requirement appears to be primarily for efficient replication within the ganglion, which leads to a sixfold increase in the number of latent sites established. Further, latent sites with high genome copy number can be established in the absence of significant virus genome replication in neurons. This suggests that neurons can be infected by many HSV virions and still enter the latent state.\n          </jats:p>","is_dataset_classified":null,"base_score":4.406719247264253,"endowment":4.406719247264253,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10623759","pmcid":"PMC111617","openalex_id":"https://openalex.org/W2102473073","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"AI32121","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI032121","title":null}],"total_grants":2,"fwci":3.259,"citation_percentile":0.91668459,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":5},{"year":2014,"count":2},{"year":2015,"count":4},{"year":2016,"count":3},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":7},{"year":2020,"count":3},{"year":2021,"count":8},{"year":2022,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.74.2.965-974.2000","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.74.2.965-974.2000","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10623759","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC112490","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/111617","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/112490","host_type":"repository"}],"fields_of_study":["Herpesvirus Infections and Treatments","Cytomegalovirus and herpesvirus research","Poxvirus research and outbreaks"],"mesh_terms":["Animals","Capsid","DNA Replication","DNA, Viral","Humans","Male","Neurons","Rabbits","Trigeminal Ganglion","Thymidine Kinase","Viral Proteins","Virus Replication","Genome, Viral","Gene Deletion","Herpes Simplex Virus Protein Vmw65","Virus Latency","Immediate-Early Proteins","Herpesvirus 1, Human","Gene Dosage","Capsid Proteins","Mice"],"keywords":["Biology","Herpes simplex virus","Viral replication","Virus latency","Virology","DNA replication","Mutant","Virus","Origin of replication","Trigeminal ganglion","Latent Virus","Thymidine kinase","Genetics","DNA","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T17:20:40.185967Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}