{"doi":"10.1128/jvi.41.1.88-96.1982","title":"Demonstration of pyrimidine dimer-DNA glycosylase activity in vivo: bacteriophage T4-infected Escherichia coli as a model system","abstract":"<jats:p>An approach to the detection of pyrimidine dimer-DNA glycosylase activity in living cells is presented. Mutants of Escherichia coli defective in uvr functions required for incision of UV-irradiated DNA were infected with phage T4 denV+ or denV- (defective in the T4 pyrimidine dimer-DNA glycosylase activity). In the former case the denV gene product catalyzed the incision of UV-irradiated host DNA, facilitating the subsequent excision of thymine-containing pyrimidine dimers. Isolation of these dimers from the acid-soluble fraction of infected cells was achieved by a multistep thin-layer chromatographic system. Exposure of the dimers to irradiation that monomerizes pyrimidine dimers (direct photoreversal) resulted in the stoichiometric formation of free thymine. Thus, in vivo incision of UV-irradiated DNA dependent on a pyrimidine dimer-DNA glycosylase can be demonstrated.</jats:p>","journal":"Journal of Virology","year":1982,"id":36329,"datarank":1.189421383011498,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.7644393814030653,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.7644393814030653,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":13,"citers_with_citation_signal":12,"citers_with_endowment":12,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":48.75,"fair_percentile":44.94283201407212,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":184250,"name":"E C 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System","DNA Glycosylases","DNA Repair","DNA, Bacterial","Escherichia coli","N-Glycosyl Hydrolases","Pyrimidine Dimers","T-Phages","Ultraviolet Rays"],"mesh_terms":["Cell-Free System","DNA Repair","DNA, Bacterial","Escherichia coli","N-Glycosyl Hydrolases","Pyrimidine Dimers","T-Phages","Ultraviolet Rays","DNA Glycosylases"],"keywords":["Pyrimidine dimer","DNA glycosylase","Biology","Thymine","DNA","Escherichia coli","Pyrimidine","Bacteriophage","Biochemistry","Dimer","Molecular biology","DNA 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