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The assay has also been useful for the isolation of nonproducer cells infected with various replication-defective transforming viruses.</jats:p>","journal":"Journal of Virology","year":1981,"id":659574,"datarank":0.9109566801150609,"base_score":6.073044534100405,"endowment":6.073044534100405,"self_citation_contribution":0.9109566801150609,"citation_network_contribution":0.0,"self_endowment_contribution":0.9109566801150609,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":433,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1721783,"name":"P Traktman","orcid":null,"position":1,"is_corresponding":false},{"id":118902,"name":"D Baltimore","orcid":null,"position":2,"is_corresponding":false},{"id":1721779,"name":"S Goff","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase","abstract":"<jats:p>A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Moloney murine leukemia virus have been isolated which (i) produce a thermolabile reverse transcriptase, (ii) are temperature sensitive for release of enzyme activity, or (iii) can only productively infect cells already producing gag-related polypeptides. 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