{"doi":"10.1128/jvi.06744-11","title":"The Human Respiratory Syncytial Virus Matrix Protein Is Required for Maturation of Viral Filaments","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>An experimental system was developed to generate infectious human respiratory syncytial virus (HRSV) lacking matrix (M) protein expression (M-null virus) from cDNA. The role of the M protein in virus assembly was then examined by infecting HEp-2 and Vero cells with the M-null virus and assessing the impact on infectious virus production and viral protein trafficking. In the absence of M, the production of infectious progeny was strongly impaired. Immunofluorescence (IF) microscopy analysis using antibodies against the nucleoprotein (N), attachment protein (G), and fusion protein (F) failed to detect the characteristic virus-induced cell surface filaments, which are believed to represent infectious virions. In addition, a large proportion of the N protein was detected in viral replication factories termed inclusion bodies (IBs). High-resolution analysis of the surface of M-null virus-infected cells by field emission scanning electron microscopy (SEM) revealed the presence of large areas with densely packed, uniformly short filaments. Although unusually short, these filaments were otherwise similar to those induced by an M-containing control virus, including the presence of the viral G and F proteins. The abundance of the short, stunted filaments in the absence of M indicates that M is not required for the initial stages of filament formation but plays an important role in the maturation or elongation of these structures. In addition, the absence of mature viral filaments and the simultaneous increase in the level of the N protein within IBs suggest that the M protein is involved in the transport of viral ribonucleoprotein (RNP) complexes from cytoplasmic IBs to sites of budding.</jats:p>","journal":"Journal of Virology","year":2012,"id":20444,"datarank":3.1826325994227473,"base_score":4.663439094112067,"endowment":4.663439094112067,"self_citation_contribution":0.6995158641168101,"citation_network_contribution":2.4831167353059374,"self_endowment_contribution":0.6995158641168101,"citer_contribution":2.4831167353059374,"corpus_percentile":null,"corpus_rank":null,"citation_count":105,"citer_count":88,"citers_with_citation_signal":68,"citers_with_endowment":68,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":135096,"name":"Pradyumna Baviskar","orcid":null,"position":1,"is_corresponding":false},{"id":135097,"name":"Rebecca R. Duncan-Decocq","orcid":null,"position":2,"is_corresponding":false},{"id":135098,"name":"Darshna Patel","orcid":null,"position":3,"is_corresponding":false},{"id":135099,"name":"Antonius G. P. Oomens","orcid":null,"position":4,"is_corresponding":false},{"id":135095,"name":"Ruchira Mitra","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.663439094112067,"endowment":4.663439094112067,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22318136","pmcid":"PMC3318654","openalex_id":"https://openalex.org/W2166677815","authors":[],"funders":[],"total_grants":0,"fwci":4.7374,"citation_percentile":0.95390046,"influential_citations":7,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":10},{"year":2014,"count":4},{"year":2015,"count":5},{"year":2016,"count":7},{"year":2017,"count":10},{"year":2018,"count":7},{"year":2019,"count":3},{"year":2020,"count":8},{"year":2021,"count":7},{"year":2022,"count":10},{"year":2023,"count":8},{"year":2024,"count":10},{"year":2025,"count":8},{"year":2026,"count":3}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3318654","host_type":"repository"},{"url":"https://jvi.asm.org/content/jvi/86/8/4432.full.pdf","host_type":"BRONZE"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3318654","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.06744-11","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.06744-11","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22318136","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.905.881","host_type":""}],"fields_of_study":["Respiratory viral infections research","Virus-based gene therapy research","Bacteriophages and microbial interactions","Biology","Medicine","Animals","Cell Line","Cell Surface Extensions","Chlorocebus aethiops","Codon","Gene Expression","Gene Order","Humans","Mutation","Open Reading Frames","Protein Transport","Respiratory Syncytial Virus, Human","Viral Matrix Proteins","Virus Replication"],"mesh_terms":["Animals","Cell Line","Chlorocebus aethiops","Codon","Humans","Mutation","Viral Matrix Proteins","Virus Replication","Gene Expression","Open Reading Frames","Respiratory Syncytial Virus, Human","Protein Transport","Cell Surface Extensions","Gene Order"],"keywords":["Biology","Viral matrix protein","Virus","Virology","Viral tegument","Viral replication","Nucleoprotein","Viral structural protein","Ribonucleoprotein","Viral entry","Viral protein","VP40","Cell biology","Molecular biology","RNA","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-04T13:36:03.163291Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}