{"doi":"10.1128/jvi.06222-11","title":"The Role of Evolutionary Intermediates in the Host Adaptation of Canine Parvovirus","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The adaptation of viruses to new hosts is a poorly understood process likely involving a variety of viral structures and functions that allow efficient replication and spread. Canine parvovirus (CPV) emerged in the late 1970s as a host-range variant of a virus related to feline panleukopenia virus (FPV). Within a few years of its emergence in dogs, there was a worldwide replacement of the initial virus strain (CPV type 2) by a variant (CPV type 2a) characterized by four amino acid differences in the capsid protein. However, the evolutionary processes that underlie the acquisition of these four mutations, as well as their effects on viral fitness, both singly and in combination, are still uncertain. Using a comprehensive experimental analysis of multiple intermediate mutational combinations, we show that these four capsid mutations act in concert to alter antigenicity, cell receptor binding, and relative\n            <jats:italic>in vitro</jats:italic>\n            growth in feline cells. Hence, host adaptation involved complex interactions among both surface-exposed and buried capsid mutations that together altered cell infection and immune escape properties of the viruses. Notably, most intermediate viral genotypes containing different combinations of the four key amino acids possessed markedly lower fitness than the wild-type viruses.\n          </jats:p>","journal":"Journal of Virology","year":2012,"id":594389,"datarank":0.6238324625039509,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"self_citation_contribution":0.6238324625039509,"citation_network_contribution":0.0,"self_endowment_contribution":0.6238324625039509,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":63,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1521532,"name":"Israel Pagan","orcid":null,"position":1,"is_corresponding":false},{"id":456329,"name":"Javier O. 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Stucker","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Role of Evolutionary Intermediates in the Host Adaptation of Canine Parvovirus","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The adaptation of viruses to new hosts is a poorly understood process likely involving a variety of viral structures and functions that allow efficient replication and spread. Canine parvovirus (CPV) emerged in the late 1970s as a host-range variant of a virus related to feline panleukopenia virus (FPV). Within a few years of its emergence in dogs, there was a worldwide replacement of the initial virus strain (CPV type 2) by a variant (CPV type 2a) characterized by four amino acid differences in the capsid protein. However, the evolutionary processes that underlie the acquisition of these four mutations, as well as their effects on viral fitness, both singly and in combination, are still uncertain. Using a comprehensive experimental analysis of multiple intermediate mutational combinations, we show that these four capsid mutations act in concert to alter antigenicity, cell receptor binding, and relative\n            <jats:italic>in vitro</jats:italic>\n            growth in feline cells. Hence, host adaptation involved complex interactions among both surface-exposed and buried capsid mutations that together altered cell infection and immune escape properties of the viruses. Notably, most intermediate viral genotypes containing different combinations of the four key amino acids possessed markedly lower fitness than the wild-type viruses.\n          </jats:p>","is_dataset_classified":null,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22114336","pmcid":"PMC3264339","openalex_id":"https://openalex.org/W2164395346","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI092571","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"RR007059","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM080533","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI092571","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI28385","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"T32 RR007059","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM080533","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI028385","title":null},{"funder_name":"European Commission","grant_id":"236470","title":"ANALYSIS OF SPECIATION MECHANISMS IN RNA VIRUSES"}],"total_grants":9,"fwci":2.4075,"citation_percentile":0.8926715,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":6},{"year":2014,"count":3},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":5},{"year":2018,"count":1},{"year":2019,"count":5},{"year":2020,"count":9},{"year":2021,"count":6},{"year":2022,"count":7},{"year":2023,"count":6},{"year":2024,"count":6},{"year":2026,"count":2}],"oa_status":"bronze","license":"ASM Journals Non-Commercial TDM","oa_locations":[{"url":"https://jvi.asm.org/content/jvi/86/3/1514.full.pdf","host_type":"journal"},{"url":"https://jvi.asm.org/content/jvi/86/3/1514.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.06222-11","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.06222-11","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22114336","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3264339","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc3264339?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.1128/jvi.06222-11","host_type":""},{"url":"http://dx.doi.org/10.1128/JVI.06222-11","host_type":""}],"fields_of_study":["Virus-based gene therapy research","Animal Virus Infections Studies","Viral gastroenteritis research and epidemiology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Adaptation, Physiological","Animals","Antigens, Viral","Base Sequence","Cats","Cell Line","DNA Primers","Dogs","Evolution, Molecular","Mutation","Parvovirus, Canine","Polymerase Chain Reaction"],"mesh_terms":["Adaptation, Physiological","Animals","Antigens, Viral","Base Sequence","Cats","Cell Line","Dogs","Mutation","Polymerase Chain Reaction","DNA Primers","Parvovirus, Canine","Evolution, Molecular"],"keywords":["Canine parvovirus","Biology","Capsid","Viral evolution","Virus","Virology","Viral replication","Parvovirus","Adaptation (eye)","Host adaptation","Host (biology)","Mutation","Genetics","Antigenicity","Viral protein","Parvoviridae","Phenotype","Viral entry","RNA","Gene","Genome","Antibody","Base Sequence","Parvovirus, Canine","Adaptation, Physiological","Polymerase Chain Reaction","Cell Line","Evolution, Molecular","Dogs","Cats","Animals","Antigens, Viral","DNA Primers"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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