{"doi":"10.1128/jvi.05071-11","title":"Construction of a Lytically Replicating Kaposi's Sarcoma-Associated Herpesvirus","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>Karposi's sarcoma-associated herpesvirus (KSHV) is found predominantly in a latent state in most cell types, impeding investigations of the lytic replication cycle. Here, we engineered the cloned KSHV genome, bacterial artificial chromosome 36 (BAC36), to enforce constitutive expression of the main lytic switch regulator, the replication and transcription activator (RTA) (open reading frame 50 [ORF50]). The resulting virus, KSHV-lyt, activated by default the lytic cycle and replicated to high titers in various cells. Using KSHV-lyt, we showed that ORF33 (encoding a tegument protein) is essential for lytic KSHV replication in cell culture, but ORF73 (encoding the latent nuclear antigen [LANA]) is not. Thus, KSHV-lyt should be highly useful to study viral gene function during lytic replication.</jats:p>","journal":"Journal of Virology","year":2011,"id":35170,"datarank":1.4061497131654976,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"self_citation_contribution":0.519860385419959,"citation_network_contribution":0.8862893277455386,"self_endowment_contribution":0.519860385419959,"citer_contribution":0.8862893277455386,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":24,"citers_with_citation_signal":21,"citers_with_endowment":21,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":180724,"name":"Tsvetana Hristozova","orcid":null,"position":1,"is_corresponding":false},{"id":180725,"name":"Georg Hille","orcid":null,"position":2,"is_corresponding":false},{"id":180726,"name":"Katrin Berger","orcid":null,"position":3,"is_corresponding":false},{"id":180727,"name":"Wolfram Brune","orcid":null,"position":4,"is_corresponding":false},{"id":180723,"name":"Matthias Budt","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21795351","pmcid":"PMC3196422","openalex_id":"https://openalex.org/W2166938188","authors":[],"funders":[],"total_grants":0,"fwci":1.7032,"citation_percentile":0.85327991,"influential_citations":1,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":5},{"year":2014,"count":3},{"year":2015,"count":7},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3196422","host_type":"repository"},{"url":"https://doi.org/10.1128/jvi.05071-11","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3196422","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.05071-11","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21795351","host_type":"repository"}],"fields_of_study":["Viral-associated cancers and disorders","Cytomegalovirus and herpesvirus research","Herpesvirus Infections and Treatments","Medicine","Biology","Cell Line","Chromosomes, Artificial, Bacterial","Gene Expression Regulation, Viral","Genes, Essential","Genes, Viral","Genetic Engineering","Herpesvirus 8, Human","Humans","Immediate-Early Proteins","Trans-Activators","Viral Load","Virulence","Virus Latency"],"mesh_terms":["Cell Line","Genes, Viral","Genetic Engineering","Humans","Virulence","Trans-Activators","Gene Expression Regulation, Viral","Virus Latency","Immediate-Early Proteins","Herpesvirus 8, Human","Viral Load","Genes, Essential","Chromosomes, Artificial, Bacterial"],"keywords":["Lytic cycle","Biology","Kaposi's sarcoma-associated herpesvirus","Virology","Gammaherpesvirinae","Viral replication","Open reading frame","Virus","Virus latency","Gene","Herpesviridae","Genetics","Viral disease"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T01:53:58.270868Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}