{"doi":"10.1128/jvi.02417-09","title":"A Role for the C Terminus of Mopeia Virus Nucleoprotein in Its Incorporation into Z Protein-Induced Virus-Like Particles","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>Arenaviruses are enveloped, negative-strand RNA viruses. For several arenaviruses, virus-like particle (VLP) formation requires the viral matrix Z protein. However, the mechanism by which viral ribonucleoprotein complexes are incorporated into virions is poorly understood. Here, we show that the expression of the Z protein and nucleoprotein (NP) of Mopeia virus, a close relative of the pathogenic Lassa virus, resulted in the highly selective incorporation of the NP protein into Z protein-induced VLPs. Moreover, the Z protein promoted the association of NP with cellular membranes, suggesting that the association of NP, Z, and the cellular membranes may facilitate the efficient incorporation of NP into VLPs. By employing a series of NP deletion constructs and testing their VLP incorporation, we further demonstrated an important role for the C-terminal half of NP in its incorporation into VLPs.</jats:p>","journal":"Journal of Virology","year":2010,"id":612557,"datarank":0.5983476069846413,"base_score":3.9889840465642745,"endowment":3.9889840465642745,"self_citation_contribution":0.5983476069846413,"citation_network_contribution":0.0,"self_endowment_contribution":0.5983476069846413,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":53,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":615274,"name":"Masaki Imai","orcid":"0000-0001-6988-1975","position":1,"is_corresponding":false},{"id":1577303,"name":"Hideo Goto","orcid":null,"position":2,"is_corresponding":false},{"id":117523,"name":"Igor S. Lukashevich","orcid":null,"position":3,"is_corresponding":false},{"id":325374,"name":"Gabriele Neumann","orcid":"0000-0001-7054-1775","position":4,"is_corresponding":false},{"id":641886,"name":"Tokiko Watanabe","orcid":"0000-0002-8242-7539","position":5,"is_corresponding":false},{"id":107110,"name":"Yoshihiro Kawaoka","orcid":"0000-0001-5061-8296","position":6,"is_corresponding":false},{"id":361664,"name":"Olena Shtanko","orcid":"0000-0002-9848-8870","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Role for the C Terminus of Mopeia Virus Nucleoprotein in Its Incorporation into Z Protein-Induced Virus-Like Particles","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>Arenaviruses are enveloped, negative-strand RNA viruses. For several arenaviruses, virus-like particle (VLP) formation requires the viral matrix Z protein. However, the mechanism by which viral ribonucleoprotein complexes are incorporated into virions is poorly understood. Here, we show that the expression of the Z protein and nucleoprotein (NP) of Mopeia virus, a close relative of the pathogenic Lassa virus, resulted in the highly selective incorporation of the NP protein into Z protein-induced VLPs. Moreover, the Z protein promoted the association of NP with cellular membranes, suggesting that the association of NP, Z, and the cellular membranes may facilitate the efficient incorporation of NP into VLPs. By employing a series of NP deletion constructs and testing their VLP incorporation, we further demonstrated an important role for the C-terminal half of NP in its incorporation into VLPs.</jats:p>","is_dataset_classified":null,"base_score":3.9889840465642745,"endowment":3.9889840465642745,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20200234","pmcid":null,"openalex_id":"https://openalex.org/W2160397329","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI055625","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI055625","title":null}],"total_grants":2,"fwci":3.8464,"citation_percentile":0.94224285,"influential_citations":0,"citation_trend":[{"year":2012,"count":8},{"year":2013,"count":7},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":2},{"year":2017,"count":3},{"year":2018,"count":3},{"year":2019,"count":4},{"year":2020,"count":2},{"year":2021,"count":2},{"year":2023,"count":4},{"year":2025,"count":3}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://jvi.asm.org/content/jvi/84/10/5415.full.pdf","host_type":"journal"},{"url":"https://jvi.asm.org/content/jvi/84/10/5415.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.02417-09","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.02417-09","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20200234","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2863806","host_type":"repository"}],"fields_of_study":["Viral Infections and Outbreaks Research","Viral Infections and Vectors","Mosquito-borne diseases and control","Animals","Arenaviruses, Old World","Cell Line","Cell Membrane","Chlorocebus aethiops","Humans","Nucleoproteins","Protein Interaction Mapping","Sequence Deletion","Viral Matrix Proteins","Virosomes","Virus Assembly"],"mesh_terms":["Animals","Cell Line","Cell Membrane","Chlorocebus aethiops","Humans","Nucleoproteins","Viral Matrix Proteins","Sequence Deletion","Arenaviruses, Old World","Virus Assembly","Virosomes","Protein Interaction Mapping"],"keywords":["Nucleoprotein","Biology","Viral matrix protein","VP40","Ribonucleoprotein","Lassa virus","Arenavirus","Virus","Virology","RNA","Virus-like particle","Gene","Biochemistry","Lymphocytic choriomeningitis"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T04:02:46.257924Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}