{"doi":"10.1128/jvi.01323-21","title":"Natural Occurring Polymorphisms in HIV-1 Integrase and RNase H Regulate Viral Release and Autoprocessing","abstract":"Nascent HIV-1 is a noninfectious viral particle. Cleaving Gag and GagPol polyproteins in the particle by mature HIV protease (PR), the nascent virus becomes an infectious virus. PR is initially translated as an inactive embedded enzyme in a GagPol polyprotein. The embedded PR in homodimerized GagPol polyproteins catalyzes a proteolytic reaction to release the mature PR. This excision step by self-cleavage is called autoprocessing. Here, during the evaluation of the roles of naturally emerging nonsynonymous SNPs in HIV RNA, we found that autoprocessing is inhibited by Met-to-Ile change at codon 50 in integrase GagPol. Other coexisting SNPs, Ser-to-Asn change at codon 17 in integrase or Asn-to-Ser mutation at codon 79 in RNase H, recovered this defect, suggesting that autoprocessing is regulated by not only integrase but also RNase H in GagPol polyprotein.","journal":"Journal of Virology","year":2021,"id":190319,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":390601,"name":"John G. Bernbaum","orcid":"0000-0002-1529-8191","position":1,"is_corresponding":false},{"id":714186,"name":"Sylvain Laverdure","orcid":"0000-0002-8053-4891","position":2,"is_corresponding":false},{"id":519258,"name":"Jun Yang","orcid":"0000-0003-2775-8914","position":3,"is_corresponding":false},{"id":753960,"name":"Chen Qian","orcid":"0000-0002-3005-5761","position":4,"is_corresponding":false},{"id":647418,"name":"Helene Highbarger","orcid":null,"position":5,"is_corresponding":false},{"id":687974,"name":"Ming Hao","orcid":"0000-0001-7618-1934","position":6,"is_corresponding":false},{"id":431773,"name":"Hongyan Sui","orcid":"0000-0002-4341-0929","position":7,"is_corresponding":false},{"id":432708,"name":"Robin Dewar","orcid":"0000-0002-7840-3155","position":8,"is_corresponding":false},{"id":421689,"name":"Weizhong Chang","orcid":"0000-0002-1413-2763","position":9,"is_corresponding":false},{"id":60624,"name":"H. Clifford Lane","orcid":"0000-0001-9509-1045","position":10,"is_corresponding":false},{"id":421688,"name":"Tomozumi Imamichi","orcid":"0000-0002-1474-1796","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-18T23:49:22.671571Z","pmid":"34523971","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}