{"doi":"10.1128/jvi.01176-20","title":"Cross-Protection against MERS-CoV by Prime-Boost Vaccination Using Viral Spike DNA and Protein","abstract":"Coronavirus is an RNA virus with a higher mutation rate than DNA viruses. Therefore, a mutation in S-protein, which mediates viral infection by binding to a human cellular receptor, is expected to cause difficulties in vaccine development. Given that DNA-protein vaccines promote stronger cell-mediated immune responses than protein-only vaccination, we immunized mice with various combinations of DNA priming and protein boosting using the S-subunit sequences of the MERS-CoV EMC/2012 strain. We demonstrated a cross-protective effect against wild-type KOR/KNIH/002, a strain with two mutations in the S amino acids, including one in its RBD. The vaccine also provided cross-neutralization against 15 different S-pseudotyped viruses. These suggested that a vaccine targeting one variant of S can provide cross-protection against multiple viral strains with mutations in S. The regimen of DNA priming/Protein boosting can be applied to the development of other coronavirus vaccines.","journal":"Journal of Virology","year":2020,"id":79717,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9581,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":414952,"name":"Junghyun Goo","orcid":null,"position":1,"is_corresponding":false},{"id":413892,"name":"Eunji Yang","orcid":"0000-0003-2590-4551","position":2,"is_corresponding":false},{"id":414953,"name":"Dae‐Im Jung","orcid":null,"position":3,"is_corresponding":false},{"id":414954,"name":"Sena Lee","orcid":null,"position":4,"is_corresponding":false},{"id":413893,"name":"Semi Rho","orcid":"0000-0003-0531-2030","position":5,"is_corresponding":false},{"id":414955,"name":"Yuji Jeong","orcid":null,"position":6,"is_corresponding":false},{"id":414956,"name":"Young-Shin Park","orcid":null,"position":7,"is_corresponding":false},{"id":414957,"name":"Hayan Park","orcid":null,"position":8,"is_corresponding":false},{"id":414958,"name":"Young-hye Moon","orcid":null,"position":9,"is_corresponding":false},{"id":413894,"name":"Uni Park","orcid":"0000-0003-0875-2584","position":10,"is_corresponding":false},{"id":413895,"name":"Sang Hwan Seo","orcid":"0000-0002-4518-8858","position":11,"is_corresponding":false},{"id":414959,"name":"Hyeja Lee","orcid":null,"position":12,"is_corresponding":false},{"id":413896,"name":"Jae Myun Lee","orcid":"0000-0002-5273-3113","position":13,"is_corresponding":false},{"id":413897,"name":"Nam‐Hyuk Cho","orcid":"0000-0003-3673-6397","position":14,"is_corresponding":false},{"id":414960,"name":"Manki Song","orcid":null,"position":15,"is_corresponding":false},{"id":413898,"name":"Jae‐Ouk Kim","orcid":"0000-0002-4556-5553","position":16,"is_corresponding":false},{"id":413891,"name":"Jung‐Ah Choi","orcid":"0000-0002-0896-4766","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-18T21:50:57.971597Z","pmid":"32967955","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}