{"doi":"10.1128/jvi.00990-23","title":"Determination of the factors responsible for the tropism of SARS-CoV-2-related bat coronaviruses to\n            <i>Rhinolophus</i>\n            bat ACE2","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Differences in host angiotensin converting enzyme 2 (ACE2) genes may affect the host range of SARS-CoV-2-related coronaviruses (SC2r-CoVs) and further determine the tropism of host ACE2 for the infection receptor. However, the factor(s) responsible for determining the host tropism of SC2r-CoVs, which may in part be determined by the tropism of host ACE2 usage, remains unclear. Here, we use the pseudoviruses with the spike proteins of two Laotian SC2r-CoVs, BANAL-20-236 and BANAL-20-52, and the cells expressing ACE2 proteins of eight different\n            <jats:italic>Rhinolophus</jats:italic>\n            bat species to show that these two spikes have different tropisms for\n            <jats:italic>Rhinolophus</jats:italic>\n            bat ACE2. Through structural analysis and cell culture experiments, we demonstrate that this tropism is determined by residue 493 of the spike and residues 31 and 35 of ACE2. Our results suggest that SC2r-CoVs exhibit differential ACE2 tropism, which may be driven by adaptation to different\n            <jats:italic>Rhinolophus</jats:italic>\n            bat ACE2 proteins.\n          </jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE</jats:title>\n            <jats:p>\n              The efficiency of infection receptor use is the first step in determining the species tropism of viruses. After the coronavirus disease 2019 pandemic, a number of SARS-CoV-2-related coronaviruses (SC2r-CoVs) were identified in\n              <jats:italic>Rhinolophus</jats:italic>\n              bats, and some of them can use human angiotensin converting enzyme 2 (ACE2) for the infection receptor without acquiring additional mutations. This means that the potential of certain SC2r-CoVs to cause spillover from bats to humans is \"off-the-shelf.\" However, both SC2r-CoVs and\n              <jats:italic>Rhinolophus</jats:italic>\n              bat species are highly diversified, and the host tropism of SC2r-CoVs remains unclear. Here, we focus on two Laotian SC2r-CoVs, BANAL-20-236 and BANAL-20-52, and determine how the tropism of SC2r-CoVs to\n              <jats:italic>Rhinolophus</jats:italic>\n              bat ACE2 is determined at the amino acid resolution level.\n            </jats:p>\n          </jats:sec>","journal":"Journal of Virology","year":2023,"id":598360,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":153818,"name":"Yusuke Kosugi","orcid":null,"position":1,"is_corresponding":false},{"id":153819,"name":"Izumi Kimura","orcid":null,"position":2,"is_corresponding":false},{"id":153820,"name":"Kenzo Tokunaga","orcid":null,"position":3,"is_corresponding":false},{"id":258983,"name":"Jumpei Ito","orcid":"0000-0003-0440-8321","position":5,"is_corresponding":false},{"id":37911,"name":"Kei Sato","orcid":"0000-0003-4431-1380","position":6,"is_corresponding":false},{"id":404511,"name":"Keita Matsuno","orcid":"0000-0002-4205-6526","position":7,"is_corresponding":false},{"id":1444128,"name":"Naganori Nao","orcid":null,"position":8,"is_corresponding":false},{"id":772615,"name":"Hirofumi Sawa","orcid":"0000-0003-2569-2755","position":9,"is_corresponding":false},{"id":615279,"name":"Shinya Tanaka","orcid":"0000-0001-6470-3301","position":10,"is_corresponding":false},{"id":615262,"name":"Masumi Tsuda","orcid":"0000-0001-5400-5905","position":11,"is_corresponding":false},{"id":231062,"name":"Lei Wang","orcid":"0000-0002-0159-1987","position":12,"is_corresponding":false},{"id":1533183,"name":"Yoshikata Oda","orcid":null,"position":13,"is_corresponding":false},{"id":1372126,"name":"Zannatul Ferdous","orcid":"0000-0001-6366-7513","position":14,"is_corresponding":false},{"id":1444129,"name":"Kenji Shishido","orcid":null,"position":15,"is_corresponding":false},{"id":378336,"name":"Takasuke Fukuhara","orcid":"0000-0001-5471-8331","position":16,"is_corresponding":false},{"id":817253,"name":"Tomokazu Tamura","orcid":"0000-0003-1395-6610","position":17,"is_corresponding":false},{"id":615255,"name":"Rigel Suzuki","orcid":"0000-0002-2700-4867","position":18,"is_corresponding":false},{"id":1385910,"name":"Saori Suzuki","orcid":"0000-0001-5233-6604","position":19,"is_corresponding":false},{"id":1443628,"name":"Hayato Ito","orcid":"0000-0002-5748-3571","position":20,"is_corresponding":false},{"id":588478,"name":"Yu Kaku","orcid":null,"position":21,"is_corresponding":false},{"id":615276,"name":"Naoko Misawa","orcid":"0000-0001-9362-803X","position":22,"is_corresponding":false},{"id":1177128,"name":"Arnon Plianchaisuk","orcid":"0009-0004-2416-0508","position":23,"is_corresponding":false},{"id":334714,"name":"Ziyi Guo","orcid":"0000-0002-7481-0140","position":24,"is_corresponding":false},{"id":1443630,"name":"Alfredo A. Hinay","orcid":"0000-0003-4761-1514","position":25,"is_corresponding":false},{"id":616892,"name":"Keiya Uriu","orcid":null,"position":26,"is_corresponding":false},{"id":1533187,"name":"Jarel Elgin M. Tolentino","orcid":null,"position":27,"is_corresponding":false},{"id":1333348,"name":"Luo Chen","orcid":"0000-0003-2665-6086","position":28,"is_corresponding":false},{"id":1497875,"name":"Lin Pan","orcid":"0000-0003-4342-1422","position":29,"is_corresponding":false},{"id":616904,"name":"Mai Suganami","orcid":null,"position":30,"is_corresponding":false},{"id":616898,"name":"Mika Chiba","orcid":null,"position":31,"is_corresponding":false},{"id":1533189,"name":"Ryo Yoshimura","orcid":null,"position":32,"is_corresponding":false},{"id":1444133,"name":"Kyoko Yasuda","orcid":null,"position":33,"is_corresponding":false},{"id":1533192,"name":"Keiko Iida","orcid":null,"position":34,"is_corresponding":false},{"id":1533194,"name":"Naomi Ohsumi","orcid":null,"position":35,"is_corresponding":false},{"id":1533195,"name":"Adam P. Strange","orcid":null,"position":36,"is_corresponding":false},{"id":344043,"name":"Shiho Tanaka","orcid":"0000-0002-2562-9660","position":37,"is_corresponding":false},{"id":616897,"name":"Kazuhisa Yoshimura","orcid":null,"position":38,"is_corresponding":false},{"id":615257,"name":"Kenji Sadamasu","orcid":"0000-0003-0541-706X","position":39,"is_corresponding":false},{"id":615272,"name":"Mami Nagashima","orcid":"0000-0001-8069-9250","position":40,"is_corresponding":false},{"id":615271,"name":"Hiroyuki Asakura","orcid":"0000-0002-2572-6786","position":41,"is_corresponding":false},{"id":615270,"name":"Isao Yoshida","orcid":"0000-0001-5234-0507","position":42,"is_corresponding":false},{"id":615280,"name":"So Nakagawa","orcid":"0000-0003-1760-3839","position":43,"is_corresponding":false},{"id":1422778,"name":"Akifumi Takaori-Kondo","orcid":null,"position":44,"is_corresponding":false},{"id":375667,"name":"Kayoko Nagata","orcid":null,"position":45,"is_corresponding":false},{"id":615273,"name":"Ryosuke Nomura","orcid":"0000-0002-9442-5522","position":46,"is_corresponding":false},{"id":616896,"name":"Yoshihito Horisawa","orcid":null,"position":47,"is_corresponding":false},{"id":1533198,"name":"Yusuke Tashiro","orcid":null,"position":48,"is_corresponding":false},{"id":1533199,"name":"Yugo Kawai","orcid":null,"position":49,"is_corresponding":false},{"id":1045242,"name":"Kazuo Takayama","orcid":"0000-0002-1132-2457","position":50,"is_corresponding":false},{"id":1443635,"name":"Rina Hashimoto","orcid":"0000-0002-7174-4563","position":51,"is_corresponding":false},{"id":1045234,"name":"Sayaka Deguchi","orcid":"0009-0007-2392-554X","position":52,"is_corresponding":false},{"id":1443636,"name":"Yukio Watanabe","orcid":"0000-0003-1504-4244","position":53,"is_corresponding":false},{"id":1443637,"name":"Ayaka Sakamoto","orcid":"0000-0001-6922-1280","position":54,"is_corresponding":false},{"id":1444136,"name":"Naoko Yasuhara","orcid":null,"position":55,"is_corresponding":false},{"id":697979,"name":"Takao Hashiguchi","orcid":"0000-0001-7578-7571","position":56,"is_corresponding":false},{"id":1443638,"name":"Tateki Suzuki","orcid":"0000-0002-6946-3850","position":57,"is_corresponding":false},{"id":1443639,"name":"Kanako Kimura","orcid":"0000-0003-4913-7502","position":58,"is_corresponding":false},{"id":1477034,"name":"Jiei Sasaki","orcid":"0000-0002-5129-2336","position":59,"is_corresponding":false},{"id":1443640,"name":"Yukari Nakajima","orcid":"0000-0002-6401-8678","position":60,"is_corresponding":false},{"id":1444137,"name":"Hisano Yajima","orcid":"0009-0000-0065-4125","position":61,"is_corresponding":false},{"id":615254,"name":"Takashi Irie","orcid":"0000-0003-3765-8482","position":62,"is_corresponding":false},{"id":616895,"name":"Ryoko Kawabata","orcid":null,"position":63,"is_corresponding":false},{"id":1533200,"name":"Kaori Tabata","orcid":null,"position":64,"is_corresponding":false},{"id":615281,"name":"Terumasa Ikeda","orcid":"0000-0003-2869-9450","position":65,"is_corresponding":false},{"id":615256,"name":"Hesham Nasser","orcid":"0000-0001-9163-1665","position":66,"is_corresponding":false},{"id":616894,"name":"Ryo Shimizu","orcid":null,"position":67,"is_corresponding":false},{"id":1533201,"name":"M. S. T. Monira Begum","orcid":null,"position":68,"is_corresponding":false},{"id":1443641,"name":"Michael Jonathan","orcid":"0000-0001-9640-1420","position":69,"is_corresponding":false},{"id":1533202,"name":"Yuka Mugita","orcid":null,"position":70,"is_corresponding":false},{"id":1533203,"name":"Otowa Takahashi","orcid":null,"position":71,"is_corresponding":false},{"id":1533204,"name":"Kimiko Ichihara","orcid":null,"position":72,"is_corresponding":false},{"id":934686,"name":"Chihiro Motozono","orcid":"0000-0002-9027-742X","position":73,"is_corresponding":false},{"id":775207,"name":"Takamasa Ueno","orcid":"0000-0003-4852-4236","position":74,"is_corresponding":false},{"id":775673,"name":"Mako Toyoda","orcid":null,"position":75,"is_corresponding":false},{"id":615253,"name":"Akatsuki Saito","orcid":"0000-0002-2792-4399","position":76,"is_corresponding":false},{"id":1147521,"name":"Maya Shofa","orcid":"0000-0002-3577-9718","position":77,"is_corresponding":false},{"id":1533205,"name":"Yuki Shibatani","orcid":null,"position":78,"is_corresponding":false},{"id":1533206,"name":"Tomoko Nishiuchi","orcid":null,"position":79,"is_corresponding":false},{"id":374970,"name":"Kotaro Shirakawa","orcid":"0000-0002-7469-1276","position":80,"is_corresponding":false},{"id":153817,"name":"Shigeru Fujita","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Determination of the factors responsible for the tropism of SARS-CoV-2-related bat coronaviruses to\n            <i>Rhinolophus</i>\n            bat ACE2","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Differences in host angiotensin converting enzyme 2 (ACE2) genes may affect the host range of SARS-CoV-2-related coronaviruses (SC2r-CoVs) and further determine the tropism of host ACE2 for the infection receptor. However, the factor(s) responsible for determining the host tropism of SC2r-CoVs, which may in part be determined by the tropism of host ACE2 usage, remains unclear. Here, we use the pseudoviruses with the spike proteins of two Laotian SC2r-CoVs, BANAL-20-236 and BANAL-20-52, and the cells expressing ACE2 proteins of eight different\n            <jats:italic>Rhinolophus</jats:italic>\n            bat species to show that these two spikes have different tropisms for\n            <jats:italic>Rhinolophus</jats:italic>\n            bat ACE2. Through structural analysis and cell culture experiments, we demonstrate that this tropism is determined by residue 493 of the spike and residues 31 and 35 of ACE2. Our results suggest that SC2r-CoVs exhibit differential ACE2 tropism, which may be driven by adaptation to different\n            <jats:italic>Rhinolophus</jats:italic>\n            bat ACE2 proteins.\n          </jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE</jats:title>\n            <jats:p>\n              The efficiency of infection receptor use is the first step in determining the species tropism of viruses. After the coronavirus disease 2019 pandemic, a number of SARS-CoV-2-related coronaviruses (SC2r-CoVs) were identified in\n              <jats:italic>Rhinolophus</jats:italic>\n              bats, and some of them can use human angiotensin converting enzyme 2 (ACE2) for the infection receptor without acquiring additional mutations. This means that the potential of certain SC2r-CoVs to cause spillover from bats to humans is \"off-the-shelf.\" However, both SC2r-CoVs and\n              <jats:italic>Rhinolophus</jats:italic>\n              bat species are highly diversified, and the host tropism of SC2r-CoVs remains unclear. Here, we focus on two Laotian SC2r-CoVs, BANAL-20-236 and BANAL-20-52, and determine how the tropism of SC2r-CoVs to\n              <jats:italic>Rhinolophus</jats:italic>\n              bat ACE2 is determined at the amino acid resolution level.\n            </jats:p>\n          </jats:sec>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37724881","pmcid":"PMC10779674","openalex_id":null,"authors":[],"funders":[{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP223fa627001","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP223fa727002","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP22fk0108511","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP22fk0108516","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP22fk0108506","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP22fk0108146","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP21fk0108494","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP21fk0108425","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP21fk0108432","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP22fk0410039","title":null},{"funder_name":"Japan Science an Technology Agency","grant_id":"PRESTO JPMJPR22R1","title":null},{"funder_name":"Japan Science an Technology Agency","grant_id":"CREST JPMJCR20H4","title":null},{"funder_name":"Japan Science an Technology Agency","grant_id":"SPRING JPMJSP2108","title":null},{"funder_name":"Japan Society for the Promotion of Science","grant_id":"KAKENHI 20K15767","title":null},{"funder_name":"Japan Society for the Promotion of Science","grant_id":"KAKENHI 23K14526","title":null},{"funder_name":"Japan Society for the Promotion of Science","grant_id":"JPJSCCA20190008","title":null}],"total_grants":16,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1128/jvi.00990-23","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/jvi.00990-23","host_type":"publisher"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10779674/pdf/jvi.00990-23.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10779674","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10779674?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Animals","Chiroptera","Humans","Phylogeny","Tropism","Spike Glycoprotein, Coronavirus","COVID-19","Angiotensin-Converting Enzyme 2","SARS-CoV-2"],"keywords":["Coronavirus","spike","ACE2","Rhinolophus Bat","Sars-cov-2"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"refseq"},{"name":"gisaid"},{"name":"pdb"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T15:33:52.028596Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}