{"doi":"10.1128/jvi.00963-19","title":"Reverse Genetics System for a Human Group A Rotavirus","abstract":"<jats:p>Reverse genetics, an approach used to generate viruses from cloned cDNA, has increased our understanding of virus biology. Worldwide research led to the development of an entirely plasmid-based reverse genetics system for the simian RV laboratory strain. Although the technique allows generation of gene-modified recombinant RVs, biological differences between animal and human RVs mean that reverse genetics systems for human RV strains are still needed. Here, we describe a reverse genetics system for the high-yield human RV strain Odelia, which replicates efficiently and is suitable for<jats:italic>in vitro</jats:italic>molecular studies. Monoreassortant viruses between simian and human RV strains and NSP1 mutant viruses generated by the rescue system enabled study of the biological functions of viral gene segments. This human RV reverse genetics system will facilitate study of RV biology and development of vaccines and vectors.</jats:p>","journal":"Journal of Virology","year":2020,"id":612358,"datarank":0.6010999777848708,"base_score":4.007333185232471,"endowment":4.007333185232471,"self_citation_contribution":0.6010999777848708,"citation_network_contribution":0.0,"self_endowment_contribution":0.6010999777848708,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":54,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1305132,"name":"Jeffery A. Nurdin","orcid":"0000-0002-2160-4458","position":1,"is_corresponding":false},{"id":1576658,"name":"Misa Onishi","orcid":null,"position":2,"is_corresponding":false},{"id":1576659,"name":"Ryotaro Nouda","orcid":null,"position":3,"is_corresponding":false},{"id":763560,"name":"Yuta Kanai","orcid":"0000-0002-5102-6951","position":4,"is_corresponding":false},{"id":1576660,"name":"Takeshi Tajima","orcid":null,"position":5,"is_corresponding":false},{"id":646099,"name":"Hiroshi Ushijima","orcid":"0000-0003-4378-3861","position":6,"is_corresponding":false},{"id":56729,"name":"Takeshi Kobayashi","orcid":"0000-0002-5532-207X","position":7,"is_corresponding":false},{"id":910454,"name":"Takahiro Kawagishi","orcid":"0000-0002-8693-1820","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Reverse Genetics System for a Human Group A Rotavirus","abstract":"<jats:p>Reverse genetics, an approach used to generate viruses from cloned cDNA, has increased our understanding of virus biology. Worldwide research led to the development of an entirely plasmid-based reverse genetics system for the simian RV laboratory strain. Although the technique allows generation of gene-modified recombinant RVs, biological differences between animal and human RVs mean that reverse genetics systems for human RV strains are still needed. Here, we describe a reverse genetics system for the high-yield human RV strain Odelia, which replicates efficiently and is suitable for<jats:italic>in vitro</jats:italic>molecular studies. Monoreassortant viruses between simian and human RV strains and NSP1 mutant viruses generated by the rescue system enabled study of the biological functions of viral gene segments. This human RV reverse genetics system will facilitate study of RV biology and development of vaccines and vectors.</jats:p>","is_dataset_classified":null,"base_score":4.007333185232471,"endowment":4.007333185232471,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31645445","pmcid":"PMC6955264","openalex_id":"https://openalex.org/W2980892742","authors":[],"funders":[{"funder_name":"Public Foundation of the Vaccination Research Center","grant_id":"2018-38","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP18im0210610","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP18fk0108018","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP18fk0108001","title":null},{"funder_name":"MEXT | Japan Society for the Promotion of Science","grant_id":"JP18H02663","title":null},{"funder_name":"MEXT | Japan Society for the Promotion of Science","grant_id":"JP19H04835","title":null},{"funder_name":"MEXT | Japan Society for the Promotion of Science","grant_id":"JP18K19444","title":null},{"funder_name":"MEXT | Japan Society for the Promotion of Science","grant_id":"JP18K07145","title":null},{"funder_name":"MEXT | Japan Society for the Promotion of Science","grant_id":"JP18K15167","title":null},{"funder_name":"MEXT | Japan Society for the Promotion of Science","grant_id":"JP16H05360","title":null}],"total_grants":10,"fwci":3.5503,"citation_percentile":0.93622582,"influential_citations":0,"citation_trend":[{"year":2020,"count":12},{"year":2021,"count":8},{"year":2022,"count":5},{"year":2023,"count":8},{"year":2024,"count":12},{"year":2025,"count":8},{"year":2026,"count":1}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6955264","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6955264","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.00963-19","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.00963-19","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31645445","host_type":"repository"}],"fields_of_study":["Viral gastroenteritis research and epidemiology","Animal Virus Infections Studies","Virus-based gene therapy research","Animals","HEK293 Cells","Haplorhini","Humans","Mutation","Reverse Genetics","Rotavirus","Rotavirus Infections","Viral Nonstructural Proteins","Virus Replication","beta-Transducin Repeat-Containing Proteins"],"mesh_terms":["Animals","Haplorhini","Humans","Mutation","Rotavirus Infections","Rotavirus","Virus Replication","Viral Nonstructural Proteins","beta-Transducin Repeat-Containing Proteins","HEK293 Cells","Reverse Genetics"],"keywords":["Reverse genetics","Biology","Rotavirus","Simian","Genetics","Human genetics","Molecular genetics","Virology","Complementary DNA","Gene","Plasmid","Virus","Mutant","Reverse genetics system","Virus-host Interactions"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T03:02:45.118560Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}