{"doi":"10.1128/jvi.00812-18","title":"<i>In Vivo</i>\n            Knockdown of the Herpes Simplex Virus 1 Latency-Associated Transcript Reduces Reactivation from Latency","abstract":"<jats:p>Herpes simplex virus (HSV) establishes a lifelong infection and remains dormant (latent) in our nerve cells. Occasionally HSV reactivates to cause disease, with HSV-1 typically causing cold sores whereas HSV-2 is the most common cause of genital herpes. The details of how HSV reactivates are largely unknown. Most of HSV's genes are silent during latency, with the exception of RNAs made from the latency-associated transcript (LAT) region. While viruses that make less LAT do not reactivate efficiently, these viruses also do not establish latency as efficiently. Here we deliver a ribozyme that can degrade the LAT to the nerve cells of latently infected rabbits using a gene therapy vector. We show that this treatment blocks reactivation in the majority of the rabbits. This work shows that the LAT RNA is important for reactivation and suggests the potential of this treatment as a therapy for treating HSV infections.</jats:p>","journal":"Journal of Virology","year":2018,"id":673039,"datarank":0.5897738449086489,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"self_citation_contribution":0.5897738449086489,"citation_network_contribution":0.0,"self_endowment_contribution":0.5897738449086489,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1758496,"name":"Shannan D. 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Here we deliver a ribozyme that can degrade the LAT to the nerve cells of latently infected rabbits using a gene therapy vector. We show that this treatment blocks reactivation in the majority of the rabbits. This work shows that the LAT RNA is important for reactivation and suggests the potential of this treatment as a therapy for treating HSV infections.</jats:p>","is_dataset_classified":null,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29875240","pmcid":"PMC6069208","openalex_id":"https://openalex.org/W2806200195","authors":[],"funders":[{"funder_name":"HHS | National Institutes of Health","grant_id":"R01 AI48633","title":null},{"funder_name":"HHS | National Institutes of Health","grant_id":"R56 AI01174","title":null},{"funder_name":"HHS | National Institutes of Health","grant_id":"TL1 TR000066","title":null},{"funder_name":"HHS | NIH | National Eye Institute","grant_id":"P30 EY02172","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI048633","title":null},{"funder_name":"NCATS NIH HHS","grant_id":"TL1 TR001428","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R56 AI101174","title":null},{"funder_name":"Research to Prevent Blindness","grant_id":"","title":null}],"total_grants":8,"fwci":4.6607,"citation_percentile":0.95777788,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2019,"count":12},{"year":2020,"count":10},{"year":2021,"count":9},{"year":2022,"count":8},{"year":2023,"count":3},{"year":2024,"count":5},{"year":2025,"count":2}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://jvi.asm.org/content/jvi/92/16/e00812-18.full.pdf","host_type":"journal"},{"url":"https://jvi.asm.org/content/jvi/92/16/e00812-18.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.00812-18","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.00812-18","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29875240","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6069208","host_type":"repository"}],"fields_of_study":["Herpesvirus Infections and Treatments","Animal Virus Infections Studies","Animal Disease Management and Epidemiology"],"mesh_terms":["Dependovirus","Animals","Cells, Cultured","Genetic Vectors","Neurons","Rabbits","RNA, Viral","Transcription, Genetic","Virus Activation","Gene Expression Regulation, Viral","RNA, Catalytic","Virus Latency","Herpesvirus 1, Human","RNA, Long Noncoding"],"keywords":["Herpes simplex virus","Biology","Virus latency","Latency (audio)","Virology","HSL and HSV","Gene knockdown","Virus","Herpesviridae","Gene","Viral replication","Viral disease","Genetics","Gene therapy","Reactivation","Latency","Ribozymes","Lncrna"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T12:09:16.569861Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}