{"doi":"10.1128/jvi.00526-06","title":"High-Level Transgene Expression in Nonhuman Primate Liver with Novel Adeno-Associated Virus Serotypes Containing Self-Complementary Genomes","abstract":"ABSTRACT\n Adeno-associated virus (AAV) vectors are being considered for in vivo applications of gene therapy in the treatment of a variety of disorders. This study evaluates the biology of second-generation vectors based on the novel serotypes AAV7 and AAV8 and containing self-complementary genomes in the nonhuman primate liver. Stable levels of transgene expression were achieved in cynomolgus macaques and suggest efficiencies at least 2 log higher than what could be achieved with AAV2 vectors using traditional single-stranded genomes. Analysis of DNAs from tissues revealed high levels of vector in the liver that appeared proportional to the relative amounts of transgene expression.","journal":"Journal of Virology","year":2006,"id":671054,"datarank":3.9463647933556785,"base_score":4.356708826689592,"endowment":4.356708826689592,"self_citation_contribution":0.6535063240034389,"citation_network_contribution":3.2928584693522396,"self_endowment_contribution":0.6535063240034389,"citer_contribution":3.2928584693522396,"corpus_percentile":null,"corpus_rank":null,"citation_count":77,"citer_count":63,"citers_with_citation_signal":57,"citers_with_endowment":57,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":865749,"name":"You Lu","orcid":"0000-0002-0075-8167","position":1,"is_corresponding":false},{"id":683526,"name":"Xun Sun","orcid":"0000-0002-9654-9473","position":2,"is_corresponding":false},{"id":1166990,"name":"Julie Johnston","orcid":"0009-0000-4667-8296","position":3,"is_corresponding":false},{"id":269113,"name":"Roberto Calcedo","orcid":null,"position":4,"is_corresponding":false},{"id":851127,"name":"Rebecca Grant","orcid":"0000-0001-8412-8113","position":5,"is_corresponding":false},{"id":546960,"name":"James M. Wilson","orcid":"0000-0001-8627-3162","position":6,"is_corresponding":false},{"id":1753008,"name":"Guang-Ping Gao","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"High-Level Transgene Expression in Nonhuman Primate Liver with Novel Adeno-Associated Virus Serotypes Containing Self-Complementary Genomes","abstract":"ABSTRACT\n Adeno-associated virus (AAV) vectors are being considered for in vivo applications of gene therapy in the treatment of a variety of disorders. This study evaluates the biology of second-generation vectors based on the novel serotypes AAV7 and AAV8 and containing self-complementary genomes in the nonhuman primate liver. Stable levels of transgene expression were achieved in cynomolgus macaques and suggest efficiencies at least 2 log higher than what could be achieved with AAV2 vectors using traditional single-stranded genomes. Analysis of DNAs from tissues revealed high levels of vector in the liver that appeared proportional to the relative amounts of transgene expression.","is_dataset_classified":null,"base_score":4.356708826689592,"endowment":4.356708826689592,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16731960","pmcid":"PMC1472562","openalex_id":"https://openalex.org/W2102539048","authors":[],"funders":[],"total_grants":0,"fwci":5.5942,"citation_percentile":0.96230842,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":4},{"year":2014,"count":2},{"year":2015,"count":6},{"year":2016,"count":3},{"year":2017,"count":6},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"http://doi.org/10.1128/JVI.00526-06","host_type":"repository"},{"url":"http://doi.org/10.1128/JVI.00526-06","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.00526-06","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.00526-06","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16731960","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC1472562","host_type":"repository"}],"fields_of_study":["Virus-based gene therapy research","RNA Interference and Gene Delivery","Viral gastroenteritis research and epidemiology","Animals","DNA, Complementary","Dependovirus","Gene Expression","Genetic Therapy","Genetic Vectors","Genome, Viral","Liver","Macaca fascicularis","Serotyping","Transgenes"],"mesh_terms":["Dependovirus","Animals","Genetic Vectors","Liver","Macaca fascicularis","Serotyping","Genetic Therapy","Gene Expression","Genome, Viral","DNA, Complementary","Transgenes"],"keywords":["Biology","Transgene","Adeno-associated virus","Genome","Vector (molecular biology)","Virology","Genetic enhancement","Primate","Virus","Vectors in gene therapy","Gene","In vivo","Genetics","Molecular biology","Recombinant DNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T01:09:31.707868Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}