{"doi":"10.1128/jvi.00362-10","title":"Recovery of Replication-Competent Residual HIV-1 from Plasma of a Patient Receiving Prolonged, Suppressive Highly Active Antiretroviral Therapy","abstract":"<jats:title>ABSTRACT</jats:title>\n                  <jats:p>\n                    The clinical significance of persistent residual viremia in patients on prolonged highly active antiretroviral therapy (HAART) is not clear. Moreover, it remains to be demonstrated whether residual viremia consists of viruses capable of spreading infection\n                    <jats:italic>in vivo</jats:italic>\n                    upon termination of therapy. Using residual viral RNAs (vRNAs) isolated from a HAART-treated patient's plasma, we cloned full-length viral genomes and found that most of them could produce infectious, replication-competent HIVs when transfected into TZM-bl cells, suggesting that residual viruses produced in the absence of therapy can initiate fresh cycles of infection and spread in host cells. The data further indicate that residual viremia may pose a major concern with regard to the emergence of drug-resistant HIVs during periods of low adherence to therapy.\n                  </jats:p>","journal":"Journal of Virology","year":2010,"id":598769,"datarank":0.5333022092234121,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"self_citation_contribution":0.5333022092234121,"citation_network_contribution":0.0,"self_endowment_contribution":0.5333022092234121,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1534531,"name":"Juan C. Sarria","orcid":null,"position":1,"is_corresponding":false},{"id":1534533,"name":"Miles W. Cloyd","orcid":null,"position":2,"is_corresponding":false},{"id":1406131,"name":"Gautam K. Sahu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Recovery of Replication-Competent Residual HIV-1 from Plasma of a Patient Receiving Prolonged, Suppressive Highly Active Antiretroviral Therapy","abstract":"<jats:title>ABSTRACT</jats:title>\n                  <jats:p>\n                    The clinical significance of persistent residual viremia in patients on prolonged highly active antiretroviral therapy (HAART) is not clear. Moreover, it remains to be demonstrated whether residual viremia consists of viruses capable of spreading infection\n                    <jats:italic>in vivo</jats:italic>\n                    upon termination of therapy. Using residual viral RNAs (vRNAs) isolated from a HAART-treated patient's plasma, we cloned full-length viral genomes and found that most of them could produce infectious, replication-competent HIVs when transfected into TZM-bl cells, suggesting that residual viruses produced in the absence of therapy can initiate fresh cycles of infection and spread in host cells. The data further indicate that residual viremia may pose a major concern with regard to the emergence of drug-resistant HIVs during periods of low adherence to therapy.\n                  </jats:p>","is_dataset_classified":null,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20519388","pmcid":"PMC2916539","openalex_id":"https://openalex.org/W2160035471","authors":[],"funders":[{"funder_name":"NCATS NIH HHS","grant_id":"UL1 TR000071","title":null}],"total_grants":1,"fwci":1.2667,"citation_percentile":0.77286312,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":4},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":6},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2024,"count":3}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://jvi.asm.org/content/jvi/84/16/8348.full.pdf","host_type":"journal"},{"url":"https://jvi.asm.org/content/jvi/84/16/8348.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JVI.00362-10","host_type":"publisher"},{"url":"https://doi.org/10.1128/jvi.00362-10","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20519388","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2916539","host_type":"repository"}],"fields_of_study":["HIV Research and Treatment","HIV/AIDS drug development and treatment","HIV/AIDS Research and Interventions"],"mesh_terms":["Cloning, Molecular","Humans","Male","Middle Aged","Molecular Sequence Data","Phylogeny","Plasma","RNA, Viral","Transfection","Virus Replication","HIV-1","HIV Infections","Cluster Analysis","Sequence Homology","Sequence Analysis, DNA","Anti-HIV Agents","Antiretroviral Therapy, Highly Active"],"keywords":["Viremia","Viral replication","Biology","Virology","Antiretroviral therapy","Viral load","In vivo","Residual","Immunology","Lentivirus","Human immunodeficiency virus (HIV)","Virus","Viral disease","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T16:12:22.936022Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}