{"doi":"10.1128/jvi.00336-20","title":"A Virion-Based Assay for Glycoprotein Thermostability Reveals Key Determinants of Filovirus Entry and Its Inhibition","abstract":"The development of Ebola virus countermeasures is challenged by our limited understanding of cell entry, especially at the step of membrane fusion. The surface-exposed viral protein, GP, mediates membrane fusion and undergoes major structural rearrangements during this process. The stability of GP at elevated temperatures (thermostability) can provide insights into its capacity to undergo these rearrangements. Here, we describe a new assay that uses GP-specific antibodies to measure GP thermostability under a variety of conditions relevant to viral entry. We show that proteolytic cleavage and acid pH have significant effects on GP thermostability that shed light on their respective roles in viral entry. We also show that the assay can be used to study how small-molecule entry inhibitors affect GP stability. This work provides a simple and readily accessible assay to engineer stabilized GP variants for antiviral vaccines and to discover and improve drugs that act by modulating GP stability.","journal":"Journal of Virology","year":2020,"id":86961,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9387,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":262594,"name":"Anthony Wong","orcid":"0000-0002-9288-061X","position":1,"is_corresponding":false},{"id":444361,"name":"Michael Grodus","orcid":null,"position":2,"is_corresponding":false},{"id":443118,"name":"Hannah S. Recht","orcid":"0000-0003-2697-3831","position":3,"is_corresponding":false},{"id":443119,"name":"Marc C. Pulanco","orcid":"0000-0001-5685-8864","position":4,"is_corresponding":false},{"id":240439,"name":"Gorka Lasso","orcid":"0000-0001-5355-323X","position":5,"is_corresponding":false},{"id":443120,"name":"Simon J. Anthony","orcid":"0000-0003-1822-8399","position":6,"is_corresponding":false},{"id":443121,"name":"Eva Mittler","orcid":"0000-0002-5935-6314","position":7,"is_corresponding":false},{"id":109529,"name":"Rohit K. Jangra","orcid":"0000-0002-3119-0869","position":8,"is_corresponding":false},{"id":109548,"name":"Kartik Chandran","orcid":"0000-0003-0232-7077","position":9,"is_corresponding":false},{"id":109540,"name":"Robert H. Bortz","orcid":"0000-0003-1751-2152","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":null,"created_at":"2026-07-18T21:59:31.247492Z","pmid":"32611759","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}