{"doi":"10.1128/jvi.00254-23","title":"The Efficiency of p27 Cleavage during <i>In Vitro</i> Respiratory Syncytial Virus (RSV) Infection Is Cell Line and RSV Subtype Dependent","abstract":"RSV fusion protein (F) plays an important role in entry and viral fusion to the host cell. The F undergoes proteolytic cleavages releasing a 27-amino-acid peptide (p27) to become fully functional. The role of p27 in viral entry and the function of the partially cleaved F containing p27 has been overlooked. p27 is thought to destabilize the F trimers, and thus, there is need for a fully cleaved F. In this study, we detected p27 on purified RSV virions and on the surface of virus-infected HEp-2 and A549 cells for circulating RSV strains of both subtypes. Higher levels of partially cleaved F containing p27 better sustained the pre-F conformation during the temperature stress challenge. Our findings highlight that the cleavage efficiency of p27 is different between RSV subtypes and among cell lines and that the presence of p27 contributes to the stability of the pre-F conformation.","journal":"Journal of Virology","year":2023,"id":358420,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9597,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":695350,"name":"Xunyan Ye","orcid":"0000-0003-3425-9654","position":1,"is_corresponding":false},{"id":757690,"name":"Laura S. Angelo","orcid":null,"position":2,"is_corresponding":false},{"id":245874,"name":"Alexandre F. Carisey","orcid":"0000-0003-1326-2205","position":3,"is_corresponding":false},{"id":236974,"name":"Vasanthi Avadhanula","orcid":"0000-0003-4016-7588","position":4,"is_corresponding":false},{"id":236976,"name":"Pedro A. Piedra","orcid":"0000-0002-5839-0879","position":5,"is_corresponding":false},{"id":756953,"name":"Wanderson Rezende","orcid":"0000-0001-5573-5811","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T01:13:43.629667Z","pmid":"37133390","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}