{"doi":"10.1128/jcm.01128-22","title":"Development of an Interferon-Gamma Release Assay (IGRA) to Aid Diagnosis of Histoplasmosis","abstract":"infection. Assay performance as an aid for diagnosing histoplasmosis was assessed in a prospective cohort of 88 people with suspected or confirmed infection, and 44 healthy controls enrolled in two centers in North America (2013 to 2018). Antigen specificity of IFN-γ release was demonstrated using ELISpot and enzyme-linked immunosorbent assay (ELISA). Antigen-evoked, single-cell mRNA expression by memory T cells was shown using flow cytometry. The area under the receiver operating characteristic curve (AUC) was estimated at 0.89 (95% confidence interval [CI]: 78.5% to 99.9%). At optimal cutoff, sensitivity was 77.2% (95% CI: 54.6% to 92.2%) and specificity was 100% (95% CI: 89.7% to 100%). Sixteen of 44 healthy volunteers (36.4%) from a region of hyperendemicity had positive responses, suggesting detection of previously unrecognized (latent) infection. The ELISpot assay is sensitive and specific as an aid to diagnose H. capsulatum infection and disease, supporting proof of concept and further development.","journal":"Journal of Clinical Microbiology","year":2022,"id":275786,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.961,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":945354,"name":"Richard W. LaRue","orcid":"0000-0002-8554-7384","position":1,"is_corresponding":false},{"id":636713,"name":"Nitipong Permpalung","orcid":"0000-0002-0749-7342","position":2,"is_corresponding":false},{"id":945355,"name":"Sukanya Das","orcid":"0000-0003-4524-4247","position":3,"is_corresponding":false},{"id":525242,"name":"Sean X. Zhang","orcid":"0000-0003-1166-2563","position":4,"is_corresponding":false},{"id":711595,"name":"Seema Mehta Steinke","orcid":"0000-0003-2375-7922","position":5,"is_corresponding":false},{"id":863922,"name":"Yuri Bushkin","orcid":null,"position":6,"is_corresponding":false},{"id":232871,"name":"Joshua D. Nosanchuk","orcid":"0000-0001-9905-5754","position":7,"is_corresponding":false},{"id":347280,"name":"Kieren A. Marr","orcid":"0000-0002-1051-0376","position":8,"is_corresponding":false},{"id":945353,"name":"Kausik Datta","orcid":"0000-0001-8666-143X","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T00:28:21.971703Z","pmid":"36190260","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}