{"doi":"10.1128/jcm.00631-23","title":"Development of a rapid lateral flow assay for detection of anti-coccidioidal antibodies","abstract":"spp. are dimorphic fungi that are capable of infecting human and non-human mammals and can cause diverse manifestations of coccidioidomycosis or Valley fever (VF). In combination with clinical symptoms and radiographic findings, antibody-based diagnostic tests are often used to diagnose and monitor patients with VF. Chitinase 1 (CTS1) has previously been identified as the seroreactive antigen used in these diagnostic assays to detect anticoccidial IgG. Here, an indirect enzyme-linked immunosorbent assay to detect IgG to CTS1 demonstrated 165 of 178 (92.7%) patients with a positive result by immunodiffusion (ID) and/or complement fixation (CF) had antibodies to the single antigen CTS1. We then developed a rapid antibody lateral flow assay (LFA) to detect anti-CTS1 antibodies. Out of 143 samples tested, the LFA showed 92.9% positive percent agreement [95% confidence interval (CI), 84.3%-96.9%] and 97.7% negative percent agreement (95% CI, 87.9%-99.6%) with ID and CF assays. Serum or plasma from canines, macaques, and dolphins was also tested by the CTS1 LFA. Test line densities of the CTS1 LFA correlated in a linear manner with the reported CF and ID titers for human and non-human samples, respectively. This 10-min point-of-care test for the rapid detection of anti-coccidioidal antibodies could help to inform healthcare providers in real-time, potentially improving the efficiency of healthcare delivery.","journal":"Journal of Clinical Microbiology","year":2023,"id":344910,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9588,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1084269,"name":"Sergei Svarovsky","orcid":null,"position":1,"is_corresponding":false},{"id":544535,"name":"María J. González‐Moa","orcid":null,"position":2,"is_corresponding":false},{"id":1084270,"name":"Erin Kaleta","orcid":null,"position":3,"is_corresponding":false},{"id":804353,"name":"Janis E. Blair","orcid":"0000-0003-2267-1473","position":4,"is_corresponding":false},{"id":1083843,"name":"Lydia Lovato","orcid":"0009-0008-1785-1393","position":5,"is_corresponding":false},{"id":401399,"name":"Richard Grant","orcid":"0000-0001-7041-0273","position":6,"is_corresponding":false},{"id":1084271,"name":"Kyle Ross","orcid":null,"position":7,"is_corresponding":false},{"id":1083844,"name":"Barbara K. Linnehan","orcid":"0000-0003-1560-3878","position":8,"is_corresponding":false},{"id":1084272,"name":"Jenny Meegan","orcid":null,"position":9,"is_corresponding":false},{"id":1084273,"name":"Kenta S. Reilly","orcid":null,"position":10,"is_corresponding":false},{"id":1084274,"name":"Ashlyn Brown","orcid":null,"position":11,"is_corresponding":false},{"id":686358,"name":"Stacy Williams","orcid":"0000-0002-0328-6548","position":12,"is_corresponding":false},{"id":593394,"name":"Yunro Chung","orcid":"0000-0001-9125-9277","position":13,"is_corresponding":false},{"id":1054572,"name":"D. Mitchell Magee","orcid":null,"position":14,"is_corresponding":false},{"id":341787,"name":"Thomas E. Grys","orcid":"0000-0002-0503-124X","position":15,"is_corresponding":false},{"id":935162,"name":"Douglas F. Lake","orcid":"0000-0001-8118-9666","position":16,"is_corresponding":false},{"id":935161,"name":"Francisca J. Grill","orcid":"0000-0003-2532-8929","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-19T01:11:35.133818Z","pmid":"37655868","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}