{"doi":"10.1128/jb.185.20.5984-5992.2003","title":"Constitutive Activation of the\n            <i>Escherichia coli</i>\n            Pho Regulon Upregulates\n            <i>rpoS</i>\n            Translation in an Hfq-Dependent Fashion","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Regulation of the σ factor RpoS occurs at the levels of transcription, translation, and protein stability activity, and it determines whether\n            <jats:italic>Escherichia coli</jats:italic>\n            turns on or off the stationary-phase response. To better understand the regulation of RpoS, we conducted genetic screens and found that mutations in the\n            <jats:italic>pst</jats:italic>\n            locus cause accumulation of RpoS during exponential growth. The\n            <jats:italic>pst</jats:italic>\n            locus encodes for the components of the high-affinity transport system for inorganic phosphate (P\n            <jats:sub>i</jats:sub>\n            ), which is involved in sensing P\n            <jats:sub>i</jats:sub>\n            levels in the environment. When the Pst transporter is compromised (either by mutation or by P\n            <jats:sub>i</jats:sub>\n            starvation), the two-component system PhoBR activates the transcription of the Pho regulon, a subset of genes that encode proteins for transporting and metabolizing alternative phosphate sources. Our data show that strains carrying mutations which constitutively activate the Pho regulon have increased\n            <jats:italic>rpoS</jats:italic>\n            translation during exponential growth. This upregulation of\n            <jats:italic>rpoS</jats:italic>\n            translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA). The transcription of this yet-to-be-identified sRNA is regulated by the PhoBR two-component system.\n          </jats:p>","journal":"Journal of Bacteriology","year":2003,"id":626911,"datarank":0.6329261557764161,"base_score":4.219507705176107,"endowment":4.219507705176107,"self_citation_contribution":0.6329261557764161,"citation_network_contribution":0.0,"self_endowment_contribution":0.6329261557764161,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":67,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":857314,"name":"Thomas J. Silhavy","orcid":"0000-0001-7672-5153","position":1,"is_corresponding":false},{"id":710376,"name":"Natividad Ruiz","orcid":"0000-0002-6369-2206","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Constitutive Activation of the\n            <i>Escherichia coli</i>\n            Pho Regulon Upregulates\n            <i>rpoS</i>\n            Translation in an Hfq-Dependent Fashion","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Regulation of the σ factor RpoS occurs at the levels of transcription, translation, and protein stability activity, and it determines whether\n            <jats:italic>Escherichia coli</jats:italic>\n            turns on or off the stationary-phase response. To better understand the regulation of RpoS, we conducted genetic screens and found that mutations in the\n            <jats:italic>pst</jats:italic>\n            locus cause accumulation of RpoS during exponential growth. The\n            <jats:italic>pst</jats:italic>\n            locus encodes for the components of the high-affinity transport system for inorganic phosphate (P\n            <jats:sub>i</jats:sub>\n            ), which is involved in sensing P\n            <jats:sub>i</jats:sub>\n            levels in the environment. When the Pst transporter is compromised (either by mutation or by P\n            <jats:sub>i</jats:sub>\n            starvation), the two-component system PhoBR activates the transcription of the Pho regulon, a subset of genes that encode proteins for transporting and metabolizing alternative phosphate sources. Our data show that strains carrying mutations which constitutively activate the Pho regulon have increased\n            <jats:italic>rpoS</jats:italic>\n            translation during exponential growth. This upregulation of\n            <jats:italic>rpoS</jats:italic>\n            translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA). The transcription of this yet-to-be-identified sRNA is regulated by the PhoBR two-component system.\n          </jats:p>","is_dataset_classified":null,"base_score":4.219507705176107,"endowment":4.219507705176107,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"14526009","pmcid":"PMC225030","openalex_id":"https://openalex.org/W2171216164","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM034821","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R37 GM034821","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM34821","title":null}],"total_grants":3,"fwci":2.804,"citation_percentile":0.90596197,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":5},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":4},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/225030","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/225030","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JB.185.20.5984-5992.2003","host_type":"publisher"},{"url":"https://doi.org/10.1128/jb.185.20.5984-5992.2003","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/14526009","host_type":"repository"}],"fields_of_study":["Bacterial Genetics and Biotechnology","Enzyme Structure and Function","Fungal and yeast genetics research"],"mesh_terms":["Bacterial Proteins","Culture Media","Escherichia coli","Mutation","Phosphates","Sigma Factor","Transcription, Genetic","Protein Biosynthesis","Up-Regulation","Gene Expression Regulation, Bacterial","Regulon","Escherichia coli Proteins","Host Factor 1 Protein"],"keywords":["rpoS","Regulon","Biology","Sigma factor","Transcription (linguistics)","Escherichia coli","Genetics","Transcription factor","Translation (biology)","Locus (genetics)","Transfer RNA","Gene","RNA polymerase","Cell biology","RNA","Gene expression","Promoter","Messenger RNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T15:37:34.034368Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}