{"doi":"10.1128/jb.183.4.1277-1283.2001","title":"The Initiation Codon Affects Ribosome Binding and Translational Efficiency in\n            <i>Escherichia coli</i>\n            of\n            <i>c</i>\n            I mRNA with or without the 5′ Untranslated Leader","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless\n            <jats:italic>c</jats:italic>\n            I mRNA from bacteriophage λ. In a\n            <jats:italic>c</jats:italic>\n            I-\n            <jats:italic>lacZ</jats:italic>\n            translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above background levels. Addition of an untranslated\n            <jats:italic>lac</jats:italic>\n            leader and Shine-Dalgarno sequence to\n            <jats:italic>c</jats:italic>\n            I increased expression but still showed a dependence on an AUG for maximum expression.\n            <jats:italic>c</jats:italic>\n            I-\n            <jats:italic>lacZ</jats:italic>\n            mRNA with an AUG initiation codon showed a greater in vitro ribosome binding strength and a higher level of full-length in vivo mRNA, suggesting that the initiation codon is an important determinant of ribosome binding strength and translational efficiency for mRNA with or without the 5′ untranslated leader.\n          </jats:p>","journal":"Journal of Bacteriology","year":2001,"id":21002,"datarank":4.584152273555308,"base_score":4.59511985013459,"endowment":4.59511985013459,"self_citation_contribution":0.6892679775201885,"citation_network_contribution":3.894884296035119,"self_endowment_contribution":0.6892679775201885,"citer_contribution":3.894884296035119,"corpus_percentile":null,"corpus_rank":null,"citation_count":98,"citer_count":91,"citers_with_citation_signal":80,"citers_with_endowment":80,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136626,"name":"Gary R. Janssen","orcid":null,"position":1,"is_corresponding":false},{"id":136850,"name":"Sean M. O'Donnell","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.59511985013459,"endowment":4.59511985013459,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11157940","pmcid":"PMC95001","openalex_id":"https://openalex.org/W2126336673","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM45923","title":null}],"total_grants":1,"fwci":1.2799,"citation_percentile":0.79459535,"influential_citations":5,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":12},{"year":2016,"count":8},{"year":2017,"count":4},{"year":2018,"count":7},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":7},{"year":2026,"count":3}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/95001","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc95001?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/95001","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JB.183.4.1277-1283.2001","host_type":"publisher"},{"url":"https://doi.org/10.1128/jb.183.4.1277-1283.2001","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11157940","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","RNA Research and Splicing","Biology","Medicine","5' Untranslated Regions","Codon, Initiator","Escherichia coli","Lac Operon","Peptide Chain Initiation, Translational","RNA, Bacterial","RNA, Messenger","Recombinant Fusion Proteins","Ribosomes","Transcription, Genetic"],"mesh_terms":["Escherichia coli","Lac Operon","Peptide Chain Initiation, Translational","Recombinant Fusion Proteins","Ribosomes","RNA, Bacterial","RNA, Messenger","Transcription, Genetic","Codon, Initiator","5' Untranslated Regions"],"keywords":["Ribosomal binding site","Start codon","Biology","Shine-Dalgarno sequence","Five prime untranslated region","Messenger RNA","Untranslated region","Translational regulation","Eukaryotic translation","Translational efficiency","Translation (biology)","Ribosome","Molecular biology","Genetics","Gene","RNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T14:03:07.689568Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}