{"doi":"10.1128/jb.154.2.663-668.1983","title":"Aldohexuronate transport system in Erwinia carotovora","abstract":"<jats:p>The biochemical and physiological aspects of hexuronate transport in Erwinia carotovora were studied to approach the genetic regulation of the hexuronate degradative pathway in this bacterial species. An active transport system for glucuronate and galacturonate uptake exists in E. carotovora. The glucuronate entry reaction displayed saturation kinetics with an apparent Km of 0.05 mM (at 25 degrees C; pH 7). Galacturonate appeared to be a competitive inhibitor of glucuronate uptake with a Ki of 0.1 mM. Glucuronate permeation was not induced by glucuronate itself in wild-type strains. Galacturonate induced the uptake of glucuronate (about fivefold). The induced synthesis of the transport system was sensitive to catabolite repression by glucose. Mutants able to grow on glucuronate as the sole carbon source showed constitutive synthesis of the hexuronate transport system.</jats:p>","journal":"Journal of Bacteriology","year":1983,"id":631757,"datarank":1.451946368971743,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.9368482882989708,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.9368482882989708,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":18,"citers_with_citation_signal":13,"citers_with_endowment":13,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1637326,"name":"Y Quesneau","orcid":null,"position":1,"is_corresponding":false},{"id":1637329,"name":"J Robert-Baudouy","orcid":null,"position":2,"is_corresponding":false},{"id":1637324,"name":"N Hugouvieux-Cotte-Pattat","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Aldohexuronate transport system in Erwinia carotovora","abstract":"<jats:p>The biochemical and physiological aspects of hexuronate transport in Erwinia carotovora were studied to approach the genetic regulation of the hexuronate degradative pathway in this bacterial species. An active transport system for glucuronate and galacturonate uptake exists in E. carotovora. The glucuronate entry reaction displayed saturation kinetics with an apparent Km of 0.05 mM (at 25 degrees C; pH 7). Galacturonate appeared to be a competitive inhibitor of glucuronate uptake with a Ki of 0.1 mM. Glucuronate permeation was not induced by glucuronate itself in wild-type strains. Galacturonate induced the uptake of glucuronate (about fivefold). The induced synthesis of the transport system was sensitive to catabolite repression by glucose. Mutants able to grow on glucuronate as the sole carbon source showed constitutive synthesis of the hexuronate transport system.</jats:p>","is_dataset_classified":null,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6841313","pmcid":"PMC217514","openalex_id":"https://openalex.org/W1553826341","authors":[],"funders":[],"total_grants":0,"fwci":2.5527,"citation_percentile":0.89755108,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2021,"count":1},{"year":2023,"count":1}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://jb.asm.org/content/jb/154/2/663.full.pdf","host_type":"journal"},{"url":"https://jb.asm.org/content/jb/154/2/663.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/jb.154.2.663-668.1983","host_type":"publisher"},{"url":"https://doi.org/10.1128/jb.154.2.663-668.1983","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6841313","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC217514","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/217514","host_type":"repository"}],"fields_of_study":["Bacterial Genetics and Biotechnology","Plant nutrient uptake and metabolism","Enzyme Structure and Function","2,4-Dinitrophenol","Azides","Biological Transport, Active","Carbonyl Cyanide m-Chlorophenyl Hydrazone","Dinitrophenols","Erwinia","Glucose","Glucuronates","Glucuronic Acid","Hexuronic Acids","Kinetics","Sodium Azide","Uronic Acids"],"mesh_terms":["Azides","Biological Transport, Active","Carbonyl Cyanide m-Chlorophenyl Hydrazone","Dinitrophenols","Erwinia","Glucose","Glucuronates","Hexuronic Acids","Kinetics","Uronic Acids","2,4-Dinitrophenol","Sodium Azide","Glucuronic Acid"],"keywords":["Glucuronate","Erwinia","Biology","Catabolite repression","Biochemistry","Microbiology","Kinetics","Mutant","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T00:37:20.674920Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}