{"doi":"10.1128/jb.01942-06","title":"Predation by\n            <i>Bdellovibrio bacteriovorus</i>\n            HD100 Requires Type IV Pili","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Early electron microscopy and more recent studies in our laboratory of\n            <jats:italic>Bdellovibrio bacteriovorus</jats:italic>\n            cells indicated the presence of narrow fibers at the nonflagellar pole of this unusual predatory bacterium. Analysis of the\n            <jats:italic>B. bacteriovorus</jats:italic>\n            HD100 genome showed a complete set of genes potentially encoding type IV pili and an incomplete gene set for Flp pili; therefore, the role of type IV pili in the predatory life cycle of\n            <jats:italic>B. bacteriovorus</jats:italic>\n            HD100 was investigated. Alignment of the predicted PilA protein with known type IV pilins showed the characteristic conserved N terminus common to type IVa pilins. The\n            <jats:italic>pilA</jats:italic>\n            gene, encoding the type IV pilus fiber protein, was insertionally inactivated in multiple\n            <jats:italic>Bdellovibrio</jats:italic>\n            replicate cultures, and the effect upon the expression of other pilus genes was monitored by reverse transcriptase PCR. Interruption of\n            <jats:italic>pilA</jats:italic>\n            in replicate isolates abolished\n            <jats:italic>Bdellovibrio</jats:italic>\n            predatory capability in liquid prey cultures and on immobilized yellow fluorescent protein-labeled prey, but the mutants could be cultured prey independently. Expression patterns of\n            <jats:italic>pil</jats:italic>\n            genes involved in the formation of type IV pili were profiled across the predatory life cycle from attack phase predatory\n            <jats:italic>Bdellovibrio</jats:italic>\n            throughout the intraperiplasmic bdelloplast stages to prey lysis and in prey-independent growth. Taken together, the data show that type IV pili play a critical role in\n            <jats:italic>Bdellovibrio</jats:italic>\n            predation.\n          </jats:p>","journal":"Journal of Bacteriology","year":2007,"id":682453,"datarank":0.7254422860427218,"base_score":4.836281906951478,"endowment":4.836281906951478,"self_citation_contribution":0.7254422860427218,"citation_network_contribution":0.0,"self_endowment_contribution":0.7254422860427218,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":125,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":905675,"name":"Carey Lambert","orcid":"0000-0002-1844-457X","position":1,"is_corresponding":false},{"id":905674,"name":"R. Elizabeth Sockett","orcid":"0000-0002-6271-2674","position":2,"is_corresponding":false},{"id":1778200,"name":"Katy J. Evans","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Predation by\n            <i>Bdellovibrio bacteriovorus</i>\n            HD100 Requires Type IV Pili","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Early electron microscopy and more recent studies in our laboratory of\n            <jats:italic>Bdellovibrio bacteriovorus</jats:italic>\n            cells indicated the presence of narrow fibers at the nonflagellar pole of this unusual predatory bacterium. Analysis of the\n            <jats:italic>B. bacteriovorus</jats:italic>\n            HD100 genome showed a complete set of genes potentially encoding type IV pili and an incomplete gene set for Flp pili; therefore, the role of type IV pili in the predatory life cycle of\n            <jats:italic>B. bacteriovorus</jats:italic>\n            HD100 was investigated. Alignment of the predicted PilA protein with known type IV pilins showed the characteristic conserved N terminus common to type IVa pilins. The\n            <jats:italic>pilA</jats:italic>\n            gene, encoding the type IV pilus fiber protein, was insertionally inactivated in multiple\n            <jats:italic>Bdellovibrio</jats:italic>\n            replicate cultures, and the effect upon the expression of other pilus genes was monitored by reverse transcriptase PCR. Interruption of\n            <jats:italic>pilA</jats:italic>\n            in replicate isolates abolished\n            <jats:italic>Bdellovibrio</jats:italic>\n            predatory capability in liquid prey cultures and on immobilized yellow fluorescent protein-labeled prey, but the mutants could be cultured prey independently. Expression patterns of\n            <jats:italic>pil</jats:italic>\n            genes involved in the formation of type IV pili were profiled across the predatory life cycle from attack phase predatory\n            <jats:italic>Bdellovibrio</jats:italic>\n            throughout the intraperiplasmic bdelloplast stages to prey lysis and in prey-independent growth. Taken together, the data show that type IV pili play a critical role in\n            <jats:italic>Bdellovibrio</jats:italic>\n            predation.\n          </jats:p>","is_dataset_classified":null,"base_score":4.836281906951478,"endowment":4.836281906951478,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17416646","pmcid":"PMC1913455","openalex_id":"https://openalex.org/W2103748135","authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"AL/067712","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":2,"fwci":2.798,"citation_percentile":0.90822035,"influential_citations":0,"citation_trend":[{"year":2012,"count":8},{"year":2013,"count":4},{"year":2014,"count":8},{"year":2015,"count":6},{"year":2016,"count":2},{"year":2017,"count":9},{"year":2018,"count":2},{"year":2019,"count":4},{"year":2020,"count":12},{"year":2021,"count":11},{"year":2022,"count":4},{"year":2023,"count":9},{"year":2024,"count":7},{"year":2025,"count":7},{"year":2026,"count":5}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1913455","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1913455","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JB.01942-06","host_type":"publisher"},{"url":"https://doi.org/10.1128/jb.01942-06","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17416646","host_type":"repository"}],"fields_of_study":["Bacterial biofilms and quorum sensing","Vibrio bacteria research studies","Genomics and Phylogenetic Studies"],"mesh_terms":["Amino Acid Sequence","Bacterial Proteins","Bdellovibrio","Genes, Bacterial","Molecular Sequence Data","Operon","Fimbriae, Bacterial","Gene Expression Regulation, Bacterial","Sequence Homology, Amino Acid","Reverse Transcriptase Polymerase Chain Reaction","Gene Order","Microscopy, Electron, Transmission"],"keywords":["Pilus","Bdellovibrio","Biology","Microbiology","Gene","Bacteria","Genetics","Virulence"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Responsible consumption and production"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T20:26:22.211052Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}