{"doi":"10.1128/jb.00518-21","title":"Natural Transformation Protein ComFA Exhibits Single-Stranded DNA Translocase Activity","abstract":"Competence, or the ability of bacteria to take up and incorporate foreign DNA in a process called natural transformation, is common in the bacterial kingdom. Research in several bacterial species suggests that long, contiguous stretches of DNA are imported into cells in a processive manner, but how bacteria power transformation remains unclear. Our finding that ComFA, a DEAD box helicase required for competence in Gram-positive bacteria, translocates on single-stranded DNA from 5' to 3', supports the long-held hypothesis that ComFA may be the motor powering DNA transport during natural transformation. Moreover, ComFA may be a previously unidentified type of DEAD box helicase-one with the capability of extended translocation on single-stranded DNA.","journal":"Journal of Bacteriology","year":2022,"id":266981,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9526,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":834126,"name":"Xiaoxuan Lin","orcid":"0000-0001-5356-9135","position":1,"is_corresponding":false},{"id":502662,"name":"Sriram Srikant","orcid":"0000-0003-3904-0336","position":2,"is_corresponding":false},{"id":475674,"name":"Rachel R. Cueny","orcid":"0000-0002-7388-6864","position":3,"is_corresponding":false},{"id":834295,"name":"Tanya G. Falbel","orcid":null,"position":4,"is_corresponding":false},{"id":475676,"name":"James L. Keck","orcid":"0000-0002-5961-0220","position":5,"is_corresponding":false},{"id":502663,"name":"Rachelle Gaudet","orcid":"0000-0002-9177-054X","position":6,"is_corresponding":false},{"id":927509,"name":"Briana M. Burton","orcid":"0000-0002-6444-8213","position":7,"is_corresponding":false},{"id":258183,"name":"Hannah R. Foster","orcid":"0000-0003-4683-6180","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T00:27:02.050512Z","pmid":"35041498","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}