{"doi":"10.1128/jb.00465-18","title":"Analysis of Pilin Antigenic Variation in Neisseria meningitidis by Next-Generation Sequencing","abstract":"<jats:p>\n            Antigenic variation is a strategy used by many pathogens to escape host immune surveillance and establish persistent infections. This study successfully applies next-generation sequencing to study pilin antigenic variation in the human pathogen\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis</jats:named-content>\n            . This assay provides an affordable and efficient solution for quantifying antigenic variation frequency in mutant strains and for defining the recombination products of the process. We determined that there is a nonuniformity of silent donor copies used during meningococcus antigenic variation, and by the analysis of selected mutants deficient for specific recombination pathways, we show for the first time that the processes are conserved between\n            <jats:named-content content-type=\"genus-species\">N. meningitidis</jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae</jats:named-content>\n            .\n          </jats:p>","journal":"Journal of Bacteriology","year":2018,"id":24503,"datarank":0.5628669476275071,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.16700834818521818,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.16700834818521818,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":9,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":146865,"name":"H Steven Seifert","orcid":"0000-0001-8651-0375","position":1,"is_corresponding":false},{"id":146864,"name":"Jing Xu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30181126","pmcid":"PMC6199478","openalex_id":"https://openalex.org/W2890293221","authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"R24378/CN001","title":null},{"funder_name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","grant_id":"R37 AI033493","title":null}],"total_grants":2,"fwci":0.8987,"citation_percentile":0.72065279,"influential_citations":0,"citation_trend":[{"year":2019,"count":4},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://jb.asm.org/content/jb/200/22/e00465-18.full.pdf","host_type":"journal"},{"url":"https://jb.asm.org/content/jb/200/22/e00465-18.full.pdf","host_type":"BRONZE"},{"url":"https://jb.asm.org/content/jb/200/22/e00465-18.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JB.00465-18","host_type":"publisher"},{"url":"https://doi.org/10.1128/jb.00465-18","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30181126","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6199478","host_type":"repository"}],"fields_of_study":["Bacterial Infections and Vaccines","Monoclonal and Polyclonal Antibodies Research","RNA and protein synthesis mechanisms","Biology","Medicine","Antigenic Variation","Antigens, Bacterial","Bacterial Proteins","Fimbriae Proteins","High-Throughput Nucleotide Sequencing","Mutation","Neisseria gonorrhoeae","Neisseria meningitidis","Phenotype"],"mesh_terms":["Antigenic Variation","Antigens, Bacterial","Bacterial Proteins","Mutation","Neisseria gonorrhoeae","Neisseria meningitidis","Phenotype","Fimbriae Proteins","High-Throughput Nucleotide Sequencing"],"keywords":["Pilin","Neisseria meningitidis","Antigenic variation","Biology","Neisseria gonorrhoeae","Phase variation","Antigen","Mutant","Neisseriaceae","Pathogen","Neisseria","Genetics","Recombination","Virology","Microbiology","Pilus","Gene","Virulence","Bacteria","Gene Conversion","454 Sequencing","Diversity Generation"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T22:33:07.040755Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}