{"doi":"10.1128/jb.00419-21","title":"Fluorescence Imaging-Based Discovery of Membrane Domain-Associated Proteins in <i>Mycobacterium smegmatis</i>","abstract":"The intracellular membrane domain (IMD) is a membrane subcompartment found in Mycobacterium smegmatis cells. Proteomic analysis of purified IMD identified more than 300 proteins, including enzymes involved in cell envelope biosynthesis. However, proteomics on its own is unlikely to detect every IMD-associated protein because of technical and biological limitations. Here, we describe fluorescent protein colocalization as an alternative, independent approach. Using a combination of fluorescence microscopy, proteomics, and subcellular fractionation, we identified three new proteins associated with the IMD. Such a robust method to rigorously define IMD proteins will benefit future investigations to decipher the synthesis, maintenance, and functions of this membrane domain and help delineate a more general mechanism of subcellular protein localization in mycobacteria.","journal":"Journal of Bacteriology","year":2021,"id":195740,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9604,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":767207,"name":"James R. Brenner","orcid":"0000-0003-1948-6672","position":1,"is_corresponding":false},{"id":678864,"name":"Alexander Dills","orcid":"0000-0002-8770-7935","position":2,"is_corresponding":false},{"id":767690,"name":"Julius J. Judd","orcid":null,"position":3,"is_corresponding":false},{"id":267215,"name":"Jemila C. Kester","orcid":"0000-0001-8639-284X","position":4,"is_corresponding":false},{"id":678336,"name":"Julia Puffal","orcid":"0000-0003-3066-5225","position":5,"is_corresponding":false},{"id":767691,"name":"Ian L. Sparks","orcid":null,"position":6,"is_corresponding":false},{"id":767692,"name":"Malavika Prithviraj","orcid":null,"position":7,"is_corresponding":false},{"id":767693,"name":"Brittany R. Anderson","orcid":null,"position":8,"is_corresponding":false},{"id":256374,"name":"Joseph T. Wade","orcid":"0000-0002-9779-3160","position":9,"is_corresponding":false},{"id":493634,"name":"Todd A. Gray","orcid":"0000-0003-0931-5503","position":10,"is_corresponding":false},{"id":493633,"name":"Keith M. Derbyshire","orcid":"0000-0003-3404-8312","position":11,"is_corresponding":false},{"id":91624,"name":"Sarah M. Fortune","orcid":"0000-0001-7565-9975","position":12,"is_corresponding":false},{"id":678337,"name":"Yasu S Morita","orcid":"0000-0002-4514-9242","position":13,"is_corresponding":false},{"id":767689,"name":"Corelle A. Z. Rokicki","orcid":null,"position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":null,"created_at":"2026-07-18T23:50:07.691929Z","pmid":"34516286","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}