{"doi":"10.1128/jb.00362-25","title":"ArgT functions as an arginine transporter in <i>Staphylococcus aureus</i>","abstract":"ABSTRACT Glycolytic activity is required for Staphylococcus aureus ( S. aureus ) to establish an infection. These data indicate that carbon catabolite repression, mediated by CcpA, is active during the initial stages of infection. CcpA represses both the biosynthesis and catabolism of arginine; therefore, it is hypothesized that arginine must be transported by S. aureus from host tissue to facilitate growth during the establishment of an infection. Within S. aureus USA300, two known arginine/ornithine antiporters, ArcD1 and ArcD2, are encoded on the two copies of the arginine deiminase operon (native and arginine catabolite mobile genetic element-derived). However, when both antiporters are inactivated via allelic replacement, no growth defect is observed in defined medium where arginine is required for growth, indicating that S. aureus contains additional arginine transporters. Using the toxic arginine analog, canavanine, we identified a novel S. aureus arginine transporter, SAUSA300_ 2383 ( Arg inine T ransporter; ArgT). Transcriptional analysis found that argT was regulated by both the canonical arginine biosynthesis repressor AhrC and CcpA; thus, its transcription is repressed during growth in medium containing glucose and is therefore not the primary arginine transporter during growth in medium containing glucose. However, we found that growth is dependent upon ArgT during growth in medium lacking proline, which suggests that S. aureus has evolved a specific response to accommodate proline-depleted growth conditions. IMPORTANCE Staphylococcus aureus is a leading cause of both community and hospital-acquired infection worldwide. In addition, S. aureus is resistant to many commonly used antibiotics, which make the treatment of bacteremia, infective endocarditis, and other invasive diseases more challenging. It is essential to obtain a basic understanding of how S. aureus survives in a variety of host niches, including those niches where S. aureus is dependent upon amino acid catabolism. We hypothesize that arginine acquisition is critical for S. aureus pathogenesis; therefore, identifying these transporters is essential for the development of novel therapeutic strategies.","journal":"Journal of Bacteriology","year":2025,"id":549023,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1241991,"name":"Itidal Reslane","orcid":null,"position":1,"is_corresponding":false},{"id":1241992,"name":"Fareha Razvi","orcid":null,"position":2,"is_corresponding":false},{"id":1241531,"name":"Luke D. Handke","orcid":"0000-0002-8758-8390","position":3,"is_corresponding":false},{"id":1241533,"name":"McKenzie K. Lehman","orcid":"0000-0002-3335-5284","position":4,"is_corresponding":false},{"id":393124,"name":"Paul D. Fey","orcid":"0000-0003-0939-6884","position":5,"is_corresponding":false},{"id":1443179,"name":"Gabrielle F. Schulze","orcid":null,"position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-19T02:54:03.053965Z","pmid":"41312975","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}