{"doi":"10.1128/jb.00251-18","title":"σ\n            <sup>I</sup>\n            from Bacillus subtilis: Impact on Gene Expression and Characterization of σ\n            <sup>I</sup>\n            -Dependent Transcription That Requires New Types of Promoters with Extended −35 and −10 Elements","abstract":"<jats:p>\n            In bacteria, σ factors are essential for transcription initiation. Knowledge about their regulons (i.e., genes transcribed from promoters dependent on these σ factors) is the key for understanding how bacteria cope with the changing environment and could be instrumental for biotechnologically motivated rewiring of gene expression. Here, we characterize the σ\n            <jats:sup>I</jats:sup>\n            regulon from the industrially important model Gram-positive bacterium\n            <jats:named-content content-type=\"genus-species\">Bacillus subtilis</jats:named-content>\n            . We reveal that σ\n            <jats:sup>I</jats:sup>\n            affects expression of ∼130 genes, of which 16 are directly regulated by σ\n            <jats:sup>I</jats:sup>\n            , including genes encoding proteins involved in iron homeostasis. Detailed analysis of promoter elements then identifies unique sequences important for σ\n            <jats:sup>I</jats:sup>\n            -dependent transcription. This study thus provides a comprehensive view on this underexplored component of the\n            <jats:named-content content-type=\"genus-species\">B. subtilis</jats:named-content>\n            transcription machinery.\n          </jats:p>","journal":"Journal of Bacteriology","year":2018,"id":684208,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1787475,"name":"Martin Převorovský","orcid":null,"position":1,"is_corresponding":false},{"id":571002,"name":"Jiří Pospíšil","orcid":"0000-0002-4720-5439","position":2,"is_corresponding":false},{"id":1787476,"name":"Dragana Vítovská","orcid":null,"position":3,"is_corresponding":false},{"id":1787477,"name":"Olga Kofroňová","orcid":null,"position":4,"is_corresponding":false},{"id":1787478,"name":"Oldřich Benada","orcid":null,"position":5,"is_corresponding":false},{"id":1787479,"name":"Marek Schwarz","orcid":null,"position":6,"is_corresponding":false},{"id":571004,"name":"Hana Šanderová","orcid":"0000-0002-5436-7583","position":7,"is_corresponding":false},{"id":571003,"name":"Jarmila Hnilicová","orcid":"0000-0002-0953-491X","position":8,"is_corresponding":false},{"id":571015,"name":"Libor Krásný","orcid":"0000-0002-4120-4347","position":9,"is_corresponding":false},{"id":1787474,"name":"Olga Ramaniuk","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"σ\n            <sup>I</sup>\n            from Bacillus subtilis: Impact on Gene Expression and Characterization of σ\n            <sup>I</sup>\n            -Dependent Transcription That Requires New Types of Promoters with Extended −35 and −10 Elements","abstract":"<jats:p>\n            In bacteria, σ factors are essential for transcription initiation. Knowledge about their regulons (i.e., genes transcribed from promoters dependent on these σ factors) is the key for understanding how bacteria cope with the changing environment and could be instrumental for biotechnologically motivated rewiring of gene expression. Here, we characterize the σ\n            <jats:sup>I</jats:sup>\n            regulon from the industrially important model Gram-positive bacterium\n            <jats:named-content content-type=\"genus-species\">Bacillus subtilis</jats:named-content>\n            . We reveal that σ\n            <jats:sup>I</jats:sup>\n            affects expression of ∼130 genes, of which 16 are directly regulated by σ\n            <jats:sup>I</jats:sup>\n            , including genes encoding proteins involved in iron homeostasis. Detailed analysis of promoter elements then identifies unique sequences important for σ\n            <jats:sup>I</jats:sup>\n            -dependent transcription. This study thus provides a comprehensive view on this underexplored component of the\n            <jats:named-content content-type=\"genus-species\">B. subtilis</jats:named-content>\n            transcription machinery.\n          </jats:p>","is_dataset_classified":null,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29914988","pmcid":"PMC6088155","openalex_id":"https://openalex.org/W2808719899","authors":[],"funders":[{"funder_name":"Czech Health Research Council","grant_id":"17-29680A","title":null},{"funder_name":"Czech Science Foundation","grant_id":"P305/12/G034","title":null},{"funder_name":"Grant Agency of the Charles University","grant_id":"322815","title":null},{"funder_name":"C4SYS","grant_id":"LM2015055","title":null},{"funder_name":"Charles University grant","grant_id":"UNCE204013","title":null},{"funder_name":"Ministry of Education, Youth and Sports of the Czech Republic","grant_id":"LO1509","title":null},{"funder_name":"Charles University grant","grant_id":"PRIMUS/MED/26","title":null},{"funder_name":"Operational Program Prague-Competitiveness","grant_id":"CZ.2.16/3.1.00/24023","title":null}],"total_grants":8,"fwci":0.6414,"citation_percentile":0.72619827,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2020,"count":3},{"year":2023,"count":4},{"year":2024,"count":3},{"year":2025,"count":2}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://jb.asm.org/content/jb/200/17/e00251-18.full.pdf","host_type":"journal"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6088155","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/JB.00251-18","host_type":"publisher"},{"url":"https://doi.org/10.1128/jb.00251-18","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29914988","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC6088155","host_type":"repository"}],"fields_of_study":["Bacterial Genetics and Biotechnology","Enzyme Structure and Function","Protein Structure and Dynamics"],"mesh_terms":["Bacillus subtilis","Bacterial Proteins","Iron","Operon","Promoter Regions, Genetic","DNA-Directed RNA Polymerases","Sigma Factor","Transcription, Genetic","Gene Expression Regulation, Bacterial","Regulon","Transcriptome"],"keywords":["Regulon","Biology","Promoter","Sigma factor","Bacillus subtilis","Gene","RNA polymerase","Operon","Genetics","Transcription factor","Transcription (linguistics)","Gene expression","Bacterial transcription","Regulation of gene expression","RNA","Escherichia coli","Bacteria","iron metabolism","Rna-seq","Rnap"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Responsible consumption and production"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"arrayexpress"},{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T13:33:39.804498Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}