{"doi":"10.1128/iai.01281-10","title":"Involvement of Quorum Sensing and Heat-Stable Enterotoxin a in Cell Damage Caused by a Porcine Enterotoxigenic\n                    <i>Escherichia coli</i>\n                    Strain","abstract":"<jats:title>ABSTRACT</jats:title>\n                  <jats:p>\n                    Enterotoxigenic\n                    <jats:italic>Escherichia coli</jats:italic>\n                    (ETEC) strains with K88 fimbriae are often associated with the outbreaks of diarrhea in newborn and weaned piglets worldwide. In the present study, we observed that 10\n                    <jats:sup>8</jats:sup>\n                    CFU/ml of K88\n                    <jats:sup>+</jats:sup>\n                    ETEC strain JG280 caused more death of pig intestinal IPEC-J2 cells than did 10\n                    <jats:sup>9</jats:sup>\n                    CFU/ml, suggesting that ETEC-induced cell death was cell density dependent and that quorum sensing (QS) may play a role in pathogenesis. Subsequent investigations demonstrated a positive correlation between autoinducer 2 (AI-2) activity of JG280 and death of IPEC-J2 cells during the infection for up to 3 h. However, there was a negative correlation between AI-2 activity and expression of the JG280 enterotoxin genes\n                    <jats:italic>estA</jats:italic>\n                    and\n                    <jats:italic>estB</jats:italic>\n                    when IPEC-J2 cells were exposed to the pathogen at 10\n                    <jats:sup>8</jats:sup>\n                    CFU/ml. We therefore cloned the\n                    <jats:italic>luxS</jats:italic>\n                    gene (responsible for AI-2 production) from JG280 and overexpressed it in\n                    <jats:italic>E. coli</jats:italic>\n                    DH5α, because deletion of the\n                    <jats:italic>luxS</jats:italic>\n                    gene was retarded by the lack of suitable antibiotic selection markers and the resistance of this pathogen to a wide range of antibiotics. The addition of culture fluid from\n                    <jats:italic>E. coli</jats:italic>\n                    DH5α with the overexpressed\n                    <jats:italic>luxS</jats:italic>\n                    reduced cell death of IPEC-J2 cells by 10\n                    <jats:sup>8</jats:sup>\n                    CFU/ml JG280. The addition also reduced the\n                    <jats:italic>estA</jats:italic>\n                    expression by JG280. Nonpathogenic K88\n                    <jats:sup>+</jats:sup>\n                    strain JFF4, which lacks the enterotoxin genes, caused no death of IPEC-J2 cells, although it produced AI-2 activity comparable to that produced by JG280. These results suggest the involvement of AI-2-mediated quorum sensing in K88\n                    <jats:sup>+</jats:sup>\n                    ETEC pathogenesis, possibly through a negative regulation of STa production.\n                  </jats:p>","journal":"Infection and Immunity","year":2011,"id":687050,"datarank":0.5533319181170905,"base_score":3.6888794541139363,"endowment":3.6888794541139363,"self_citation_contribution":0.5533319181170905,"citation_network_contribution":0.0,"self_endowment_contribution":0.5533319181170905,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1794901,"name":"Xianhua Yin","orcid":null,"position":1,"is_corresponding":false},{"id":857265,"name":"Hai Yu","orcid":"0000-0003-2465-0801","position":2,"is_corresponding":false},{"id":640984,"name":"Liping Zhao","orcid":"0000-0001-9694-7140","position":3,"is_corresponding":false},{"id":1794904,"name":"Parviz Sabour","orcid":null,"position":4,"is_corresponding":false},{"id":1794905,"name":"Joshua Gong","orcid":null,"position":5,"is_corresponding":false},{"id":461366,"name":"Jing Zhu","orcid":"0000-0002-8014-2855","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Involvement of Quorum Sensing and Heat-Stable Enterotoxin a in Cell Damage Caused by a Porcine Enterotoxigenic\n                    <i>Escherichia coli</i>\n                    Strain","abstract":"<jats:title>ABSTRACT</jats:title>\n                  <jats:p>\n                    Enterotoxigenic\n                    <jats:italic>Escherichia coli</jats:italic>\n                    (ETEC) strains with K88 fimbriae are often associated with the outbreaks of diarrhea in newborn and weaned piglets worldwide. In the present study, we observed that 10\n                    <jats:sup>8</jats:sup>\n                    CFU/ml of K88\n                    <jats:sup>+</jats:sup>\n                    ETEC strain JG280 caused more death of pig intestinal IPEC-J2 cells than did 10\n                    <jats:sup>9</jats:sup>\n                    CFU/ml, suggesting that ETEC-induced cell death was cell density dependent and that quorum sensing (QS) may play a role in pathogenesis. Subsequent investigations demonstrated a positive correlation between autoinducer 2 (AI-2) activity of JG280 and death of IPEC-J2 cells during the infection for up to 3 h. However, there was a negative correlation between AI-2 activity and expression of the JG280 enterotoxin genes\n                    <jats:italic>estA</jats:italic>\n                    and\n                    <jats:italic>estB</jats:italic>\n                    when IPEC-J2 cells were exposed to the pathogen at 10\n                    <jats:sup>8</jats:sup>\n                    CFU/ml. We therefore cloned the\n                    <jats:italic>luxS</jats:italic>\n                    gene (responsible for AI-2 production) from JG280 and overexpressed it in\n                    <jats:italic>E. coli</jats:italic>\n                    DH5α, because deletion of the\n                    <jats:italic>luxS</jats:italic>\n                    gene was retarded by the lack of suitable antibiotic selection markers and the resistance of this pathogen to a wide range of antibiotics. The addition of culture fluid from\n                    <jats:italic>E. coli</jats:italic>\n                    DH5α with the overexpressed\n                    <jats:italic>luxS</jats:italic>\n                    reduced cell death of IPEC-J2 cells by 10\n                    <jats:sup>8</jats:sup>\n                    CFU/ml JG280. The addition also reduced the\n                    <jats:italic>estA</jats:italic>\n                    expression by JG280. Nonpathogenic K88\n                    <jats:sup>+</jats:sup>\n                    strain JFF4, which lacks the enterotoxin genes, caused no death of IPEC-J2 cells, although it produced AI-2 activity comparable to that produced by JG280. These results suggest the involvement of AI-2-mediated quorum sensing in K88\n                    <jats:sup>+</jats:sup>\n                    ETEC pathogenesis, possibly through a negative regulation of STa production.\n                  </jats:p>","is_dataset_classified":null,"base_score":3.6888794541139363,"endowment":3.6888794541139363,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21300771","pmcid":"PMC3067552","openalex_id":"https://openalex.org/W2110504024","authors":[],"funders":[],"total_grants":0,"fwci":1.318,"citation_percentile":0.78830607,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":1},{"year":2014,"count":3},{"year":2015,"count":2},{"year":2016,"count":2},{"year":2017,"count":4},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2021,"count":9},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":2}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3067552","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3067552","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/IAI.01281-10","host_type":"publisher"},{"url":"https://doi.org/10.1128/iai.01281-10","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21300771","host_type":"repository"}],"fields_of_study":["Escherichia coli research studies","Viral gastroenteritis research and epidemiology","Clostridium difficile and Clostridium perfringens research","Animals","Bacterial Proteins","Carbon-Sulfur Lyases","Enterotoxigenic Escherichia coli","Enterotoxins","Escherichia coli Infections","Gene Expression","Gene Expression Regulation, Bacterial","Homoserine","Lactones","Quorum Sensing","Reverse Transcriptase Polymerase Chain Reaction","Swine","Virulence Factors"],"mesh_terms":["Animals","Bacterial Proteins","Enterotoxins","Escherichia coli Infections","Homoserine","Lactones","Carbon-Sulfur Lyases","Swine","Gene Expression","Gene Expression Regulation, Bacterial","Reverse Transcriptase Polymerase Chain Reaction","Virulence Factors","Quorum Sensing","Enterotoxigenic Escherichia coli"],"keywords":["Enterotoxigenic Escherichia coli","Enterotoxin","Heat-stable enterotoxin","Biology","Quorum sensing","Microbiology","Strain (injury)","Escherichia coli","Heat-labile enterotoxin","Bacteria","Genetics","Gene","Biofilm"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.14738526.v1","title":"Additional file 1 of Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14738526","title":"Additional file 1 of Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T21:11:34.208450Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}