{"doi":"10.1128/iai.00864-06","title":"Carboxypeptidases B of\n            <i>Anopheles gambiae</i>\n            as Targets for a\n            <i>Plasmodium falciparum</i>\n            Transmission-Blocking Vaccine","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:italic>Anopheles gambiae</jats:italic>\n            is the major African vector of\n            <jats:italic>Plasmodium falciparum</jats:italic>\n            , the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs), which induce in the vertebrate host the production of antibodies that inhibit parasite development in the mosquito midgut. So far, the most promising components of a TBV are parasite-derived antigens, although targeting critical mosquito components might also successfully block development of the parasite in its vector. We previously identified\n            <jats:italic>A. gambiae</jats:italic>\n            genes whose expression was modified in\n            <jats:italic>P. falciparum</jats:italic>\n            -infected mosquitoes, including one midgut carboxypeptidase gene,\n            <jats:italic>cpbAg1.</jats:italic>\n            Here we show that\n            <jats:italic>P. falciparum</jats:italic>\n            up-regulates the expression of\n            <jats:italic>cpbAg1</jats:italic>\n            and of a second midgut carboxypeptidase gene,\n            <jats:italic>cpbAg2</jats:italic>\n            , and that this up-regulation correlates with an increased carboxypeptidase B (CPB) activity at a time when parasites establish infection in the mosquito midgut. The addition of antibodies directed against CPBAg1 to a\n            <jats:italic>P. falciparum</jats:italic>\n            -containing blood meal inhibited CPB activity and blocked parasite development in the mosquito midgut. Furthermore, the development of the rodent parasite\n            <jats:italic>Plasmodium berghei</jats:italic>\n            was significantly reduced in mosquitoes fed on infected mice that had been immunized with recombinant CPBAg1. Lastly, mosquitoes fed on anti-CPBAg1 antibodies exhibited reduced reproductive capacity, a secondary effect of a CPB-based TBV that could likely contribute to reducing\n            <jats:italic>Plasmodium</jats:italic>\n            transmission. These results indicate that\n            <jats:italic>A. gambiae</jats:italic>\n            CPBs could constitute targets for a TBV that is based upon mosquito molecules.\n          </jats:p>","journal":"Infection and Immunity","year":2007,"id":46468,"datarank":3.58832060788723,"base_score":4.499809670330265,"endowment":4.499809670330265,"self_citation_contribution":0.6749714505495399,"citation_network_contribution":2.9133491573376897,"self_endowment_contribution":0.6749714505495399,"citer_contribution":2.9133491573376897,"corpus_percentile":null,"corpus_rank":null,"citation_count":89,"citer_count":87,"citers_with_citation_signal":70,"citers_with_endowment":70,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":215486,"name":"C. Boudin","orcid":null,"position":1,"is_corresponding":false},{"id":215487,"name":"R. Lacroix","orcid":null,"position":2,"is_corresponding":false},{"id":215488,"name":"S. Bonnet","orcid":null,"position":3,"is_corresponding":false},{"id":215489,"name":"A. Diop","orcid":null,"position":4,"is_corresponding":false},{"id":215490,"name":"S. Thiberge","orcid":null,"position":5,"is_corresponding":false},{"id":215491,"name":"B. Boisson","orcid":null,"position":6,"is_corresponding":false},{"id":215492,"name":"R. Tahar","orcid":null,"position":7,"is_corresponding":false},{"id":215493,"name":"C. Bourgouin","orcid":null,"position":8,"is_corresponding":false},{"id":215485,"name":"C. Lavazec","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Carboxypeptidases B of\n            <i>Anopheles gambiae</i>\n            as Targets for a\n            <i>Plasmodium falciparum</i>\n            Transmission-Blocking Vaccine","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:italic>Anopheles gambiae</jats:italic>\n            is the major African vector of\n            <jats:italic>Plasmodium falciparum</jats:italic>\n            , the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs), which induce in the vertebrate host the production of antibodies that inhibit parasite development in the mosquito midgut. So far, the most promising components of a TBV are parasite-derived antigens, although targeting critical mosquito components might also successfully block development of the parasite in its vector. We previously identified\n            <jats:italic>A. gambiae</jats:italic>\n            genes whose expression was modified in\n            <jats:italic>P. falciparum</jats:italic>\n            -infected mosquitoes, including one midgut carboxypeptidase gene,\n            <jats:italic>cpbAg1.</jats:italic>\n            Here we show that\n            <jats:italic>P. falciparum</jats:italic>\n            up-regulates the expression of\n            <jats:italic>cpbAg1</jats:italic>\n            and of a second midgut carboxypeptidase gene,\n            <jats:italic>cpbAg2</jats:italic>\n            , and that this up-regulation correlates with an increased carboxypeptidase B (CPB) activity at a time when parasites establish infection in the mosquito midgut. The addition of antibodies directed against CPBAg1 to a\n            <jats:italic>P. falciparum</jats:italic>\n            -containing blood meal inhibited CPB activity and blocked parasite development in the mosquito midgut. Furthermore, the development of the rodent parasite\n            <jats:italic>Plasmodium berghei</jats:italic>\n            was significantly reduced in mosquitoes fed on infected mice that had been immunized with recombinant CPBAg1. Lastly, mosquitoes fed on anti-CPBAg1 antibodies exhibited reduced reproductive capacity, a secondary effect of a CPB-based TBV that could likely contribute to reducing\n            <jats:italic>Plasmodium</jats:italic>\n            transmission. These results indicate that\n            <jats:italic>A. gambiae</jats:italic>\n            CPBs could constitute targets for a TBV that is based upon mosquito molecules.\n          </jats:p>","is_dataset_classified":null,"base_score":4.499809670330265,"endowment":4.499809670330265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17283100","pmcid":"PMC1865713","openalex_id":"https://openalex.org/W2102770366","authors":[],"funders":[],"total_grants":0,"fwci":4.6213,"citation_percentile":0.94142788,"influential_citations":8,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":4},{"year":2014,"count":5},{"year":2015,"count":7},{"year":2016,"count":7},{"year":2017,"count":3},{"year":2018,"count":6},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":6},{"year":2022,"count":3},{"year":2023,"count":7},{"year":2024,"count":3},{"year":2025,"count":4},{"year":2026,"count":2}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1865713","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc1865713?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1865713","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/IAI.00864-06","host_type":"publisher"},{"url":"https://doi.org/10.1128/iai.00864-06","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17283100","host_type":"repository"},{"url":"https://pasteur.hal.science/pasteur-04008691","host_type":"repository"}],"fields_of_study":["Malaria Research and Control","Invertebrate Immune Response Mechanisms","Mosquito-borne diseases and control","Biology","Medicine","Environmental Science","Animals","Anopheles","Antibodies","Carboxypeptidase B","Disease Models, Animal","Female","Gastrointestinal Tract","Humans","Malaria","Malaria Vaccines","Malaria, Falciparum","Mice","Plasmodium berghei","Plasmodium falciparum","Reproduction","Up-Regulation"],"mesh_terms":["Animals","Anopheles","Antibodies","Disease Models, Animal","Female","Humans","Malaria","Plasmodium berghei","Plasmodium falciparum","Reproduction","Up-Regulation","Malaria, Falciparum","Malaria Vaccines","Gastrointestinal Tract","Carboxypeptidase B","Mice"],"keywords":["Anopheles gambiae","Biology","Midgut","Plasmodium falciparum","Parasite hosting","Plasmodium berghei","Vector (molecular biology)","Virology","Anopheles","Malaria","Plasmodium (life cycle)","Gametocyte","Recombinant DNA","Gene","Immunology","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-10T04:24:32.846467Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}