{"doi":"10.1128/iai.00338-08","title":"Identification of\n            <i>Streptococcus sanguinis</i>\n            Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:italic>Streptococcus sanguinis</jats:italic>\n            is one of the pioneers in the bacterial colonization of teeth and is one of the most abundant species in the oral biofilm called dental plaque.\n            <jats:italic>S. sanguinis</jats:italic>\n            is also the most common viridans group streptococcal species implicated in infective endocarditis. To investigate the association of biofilm and endocarditis, we established a biofilm assay and examined biofilm formation with a signature-tagged mutagenesis library of\n            <jats:italic>S. sanguinis</jats:italic>\n            . Four genes that have not previously been associated with biofilm formation in any other bacterium,\n            <jats:italic>purB, purL, thrB</jats:italic>\n            , and\n            <jats:italic>pyrE</jats:italic>\n            , were putatively identified as contributing to in vitro biofilm formation in\n            <jats:italic>S. sanguinis</jats:italic>\n            . By examining 800 mutants for attenuation in the rabbit endocarditis model and for reduction in biofilm formation in vitro, we found some mutants that were both biofilm defective and attenuated for endocarditis. However, we also identified mutants with only reduced biofilm formation or with only attenuation in the endocarditis model. This result indicates that the ability to form biofilms in vitro is not associated with endocarditis virulence in vivo in\n            <jats:italic>S. sanguinis</jats:italic>\n            .\n          </jats:p>","journal":"Infection and Immunity","year":2008,"id":599260,"datarank":0.692268077526189,"base_score":4.61512051684126,"endowment":4.61512051684126,"self_citation_contribution":0.692268077526189,"citation_network_contribution":0.0,"self_endowment_contribution":0.692268077526189,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":100,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":379069,"name":"Todd Kitten","orcid":"0000-0001-6097-7583","position":1,"is_corresponding":false},{"id":1535815,"name":"Zhenming Chen","orcid":null,"position":2,"is_corresponding":false},{"id":1535816,"name":"Sehmi P. Lee","orcid":null,"position":3,"is_corresponding":false},{"id":535284,"name":"Cindy L. Munro","orcid":"0000-0003-2799-3973","position":4,"is_corresponding":false},{"id":369303,"name":"Ping Xu","orcid":"0000-0002-4652-4841","position":5,"is_corresponding":false},{"id":1535814,"name":"Xiuchun Ge","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification of\n            <i>Streptococcus sanguinis</i>\n            Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:italic>Streptococcus sanguinis</jats:italic>\n            is one of the pioneers in the bacterial colonization of teeth and is one of the most abundant species in the oral biofilm called dental plaque.\n            <jats:italic>S. sanguinis</jats:italic>\n            is also the most common viridans group streptococcal species implicated in infective endocarditis. To investigate the association of biofilm and endocarditis, we established a biofilm assay and examined biofilm formation with a signature-tagged mutagenesis library of\n            <jats:italic>S. sanguinis</jats:italic>\n            . Four genes that have not previously been associated with biofilm formation in any other bacterium,\n            <jats:italic>purB, purL, thrB</jats:italic>\n            , and\n            <jats:italic>pyrE</jats:italic>\n            , were putatively identified as contributing to in vitro biofilm formation in\n            <jats:italic>S. sanguinis</jats:italic>\n            . By examining 800 mutants for attenuation in the rabbit endocarditis model and for reduction in biofilm formation in vitro, we found some mutants that were both biofilm defective and attenuated for endocarditis. However, we also identified mutants with only reduced biofilm formation or with only attenuation in the endocarditis model. This result indicates that the ability to form biofilms in vitro is not associated with endocarditis virulence in vivo in\n            <jats:italic>S. sanguinis</jats:italic>\n            .\n          </jats:p>","is_dataset_classified":null,"base_score":4.61512051684126,"endowment":4.61512051684126,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18390999","pmcid":"PMC2423065","openalex_id":"https://openalex.org/W2164028009","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"K02AI054908","title":null},{"funder_name":"NIDCR NIH HHS","grant_id":"R01 DE018138","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI047841","title":null},{"funder_name":"NIDCR NIH HHS","grant_id":"R01DE18138","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01AI47841","title":null}],"total_grants":5,"fwci":4.4024,"citation_percentile":0.94612014,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":7},{"year":2014,"count":6},{"year":2015,"count":2},{"year":2016,"count":9},{"year":2017,"count":4},{"year":2018,"count":5},{"year":2019,"count":9},{"year":2020,"count":10},{"year":2021,"count":4},{"year":2022,"count":4},{"year":2023,"count":9},{"year":2024,"count":3},{"year":2025,"count":3},{"year":2026,"count":2}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://iai.asm.org/content/iai/76/6/2551.full.pdf","host_type":"journal"},{"url":"https://iai.asm.org/content/iai/76/6/2551.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/IAI.00338-08","host_type":"publisher"},{"url":"https://doi.org/10.1128/iai.00338-08","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18390999","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2423065","host_type":"repository"}],"fields_of_study":["Infective Endocarditis Diagnosis and Management","Streptococcal Infections and Treatments","Oral microbiology and periodontitis research"],"mesh_terms":["Animals","Bacterial Proteins","Endocarditis, Bacterial","Genes, Bacterial","Mutation","Rabbits","Streptococcal Infections","Streptococcus sanguis","Virulence","Gene Expression Regulation, Bacterial","Biofilms"],"keywords":["Streptococcus sanguinis","Biofilm","Microbiology","Endocarditis","Virulence","Biology","Mutant","Bacteria","Gene","Genetics","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T21:01:34.527473Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}