{"doi":"10.1128/aem.70.1.167-173.2004","title":"Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria","abstract":"<jats:title>ABSTRACT</jats:title><jats:p>A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with<jats:italic>Bifidobacterium</jats:italic>genus- and species-specific primers. Real-time PCR detection of serially diluted DNA extracted from cultured bifidobacteria was linear for cell counts ranging from 10<jats:sup>6</jats:sup>to 10 cells per PCR assay. It was also found that the method was applicable to the detection of<jats:italic>Bifidobacterium</jats:italic>in feces when it was present at concentrations of &gt;10<jats:sup>6</jats:sup>cells per g of feces. Concerning the distribution of<jats:italic>Bifidobacterium</jats:italic>species in intestinal flora, the<jats:italic>Bifidobacterium adolescentis</jats:italic>group, the<jats:italic>Bifidobacterium catenulatum</jats:italic>group, and<jats:italic>Bifidobacterium longum</jats:italic>were found to be the three predominant species by examination of DNA extracted from the feces of 46 healthy adults. We also examined changes in the population and composition of<jats:italic>Bifidobacterium</jats:italic>species in human intestinal flora of six healthy adults over an 8-month period. The results showed that the composition of bifidobacterial flora was basically stable throughout the test period.</jats:p>","journal":"Applied and Environmental Microbiology","year":2004,"id":22006,"datarank":17.62460489786737,"base_score":6.218600119691729,"endowment":6.218600119691729,"self_citation_contribution":0.9327900179537595,"citation_network_contribution":16.69181487991361,"self_endowment_contribution":0.9327900179537595,"citer_contribution":16.69181487991361,"corpus_percentile":null,"corpus_rank":null,"citation_count":501,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":25,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":139735,"name":"Koichi Watanabe","orcid":null,"position":1,"is_corresponding":false},{"id":139736,"name":"Junji 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Matsuki","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":6.218600119691729,"endowment":6.218600119691729,"datacite_reuse_total":25,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"14711639","pmcid":"PMC321263","openalex_id":"https://openalex.org/W1977455677","authors":[],"funders":[],"total_grants":0,"fwci":28.4892,"citation_percentile":0.99847128,"influential_citations":34,"citation_trend":[{"year":2012,"count":35},{"year":2013,"count":25},{"year":2014,"count":26},{"year":2015,"count":28},{"year":2016,"count":26},{"year":2017,"count":26},{"year":2018,"count":24},{"year":2019,"count":20},{"year":2020,"count":24},{"year":2021,"count":31},{"year":2022,"count":41},{"year":2023,"count":19},{"year":2024,"count":14},{"year":2025,"count":17},{"year":2026,"count":2}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/321263","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc321263?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/321263","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/AEM.70.1.167-173.2004","host_type":"publisher"},{"url":"https://doi.org/10.1128/aem.70.1.167-173.2004","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/14711639","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC321263","host_type":"repository"}],"fields_of_study":["Probiotics and Fermented Foods","Gut microbiota and health","Genomics and Phylogenetic Studies","Medicine","Biology","Adult","Base Sequence","Bifidobacterium","Colony Count, Microbial","DNA Primers","DNA, Ribosomal","Female","Genes, rRNA","Humans","Intestines","Male","Middle Aged","Molecular Sequence Data","Polymerase Chain Reaction","RNA, Ribosomal, 16S","Sequence Analysis, DNA","Species Specificity"],"mesh_terms":["Adult","Base Sequence","Bifidobacterium","DNA, Ribosomal","Female","Humans","Intestines","Male","Middle Aged","Molecular Sequence Data","RNA, Ribosomal, 16S","Species Specificity","Colony Count, Microbial","Polymerase Chain Reaction","Sequence Analysis, DNA","DNA Primers","Genes, rRNA"],"keywords":["Bifidobacterium","Bifidobacterium longum","Biology","Actinomycetaceae","Feces","Microbiology","16S ribosomal RNA","Population","Human feces","Ribosomal RNA","Polymerase chain reaction","Bacteria","Gene","Genetics","Lactobacillus"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in 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