{"doi":"10.1128/aem.03016-13","title":"Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            One of the functions of the mammalian large intestinal microbiota is the fermentation of plant cell wall components. In ruminant animals, the majority of their nutrients are obtained via pregastric fermentation; however, up to 20% can be recovered from microbial fermentation in the large intestine. Eight-week continuous culture enrichments of cattle feces with cellulose and xylan-pectin were used to isolate bacteria from this community. A total of 459 bacterial isolates were classified phylogenetically using 16S rRNA gene sequencing. Six phyla were represented:\n            <jats:named-content content-type=\"genus-species\">Firmicutes</jats:named-content>\n            (51.9%),\n            <jats:named-content content-type=\"genus-species\">Bacteroidetes</jats:named-content>\n            (30.9%),\n            <jats:named-content content-type=\"genus-species\">Proteobacteria</jats:named-content>\n            (11.1%),\n            <jats:named-content content-type=\"genus-species\">Actinobacteria</jats:named-content>\n            (3.5%),\n            <jats:named-content content-type=\"genus-species\">Synergistetes</jats:named-content>\n            (1.5%), and\n            <jats:named-content content-type=\"genus-species\">Fusobacteria</jats:named-content>\n            (1.1%). The majority of bacterial isolates had &lt;98.5% identity to cultured bacteria with sequences in the Ribosomal Database Project and thus represent new species and/or genera. Within the\n            <jats:named-content content-type=\"genus-species\">Firmicutes</jats:named-content>\n            isolates, most were classified in the families\n            <jats:named-content content-type=\"genus-species\">Lachnospiraceae</jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Ruminococcaceae</jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Erysipelotrichaceae</jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Clostridiaceae</jats:named-content>\n            I. The majority of the\n            <jats:named-content content-type=\"genus-species\">Bacteroidetes</jats:named-content>\n            were most closely related to\n            <jats:named-content content-type=\"genus-species\">Bacteroides thetaiotaomicron</jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">B. ovatus</jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">B. xylanisolvens</jats:named-content>\n            and members of the\n            <jats:named-content content-type=\"genus-species\">Porphyromonadaceae</jats:named-content>\n            family. Many of the\n            <jats:named-content content-type=\"genus-species\">Firmicutes</jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Bacteroidetes</jats:named-content>\n            isolates were related to species demonstrated to possess enzymes which ferment plant cell wall components; the others were hypothesized to cross-feed these bacteria. The microbial communities that arose in these enrichment cultures had broad bacterial diversity. With over 98% of the isolates not represented as previously cultured, there are new opportunities to study the genomic and metabolic capacities of these members of the complex intestinal microbiota.\n          </jats:p>","journal":"Applied and Environmental Microbiology","year":2014,"id":33781,"datarank":3.577184743854862,"base_score":4.406719247264253,"endowment":4.406719247264253,"self_citation_contribution":0.6610078870896381,"citation_network_contribution":2.916176856765224,"self_endowment_contribution":0.6610078870896381,"citer_contribution":2.916176856765224,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":78,"citers_with_citation_signal":62,"citers_with_endowment":62,"datacite_reuse_total":6,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":176083,"name":"Cherie J. Ziemer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.406719247264253,"endowment":4.406719247264253,"datacite_reuse_total":6,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24212576","pmcid":"PMC3911107","openalex_id":"https://openalex.org/W2120061385","authors":[],"funders":[],"total_grants":0,"fwci":1.9442,"citation_percentile":0.86204024,"influential_citations":2,"citation_trend":[{"year":2014,"count":3},{"year":2015,"count":8},{"year":2016,"count":2},{"year":2017,"count":17},{"year":2018,"count":5},{"year":2019,"count":10},{"year":2020,"count":4},{"year":2021,"count":12},{"year":2022,"count":5},{"year":2023,"count":6},{"year":2024,"count":7},{"year":2026,"count":2}],"oa_status":"bronze","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://aem.asm.org/content/aem/80/2/574.full.pdf","host_type":"journal"},{"url":"https://aem.asm.org/content/aem/80/2/574.full.pdf","host_type":"GREEN"},{"url":"https://aem.asm.org/content/aem/80/2/574.full.pdf","host_type":"publisher"},{"url":"https://journals.asm.org/doi/pdf/10.1128/AEM.03016-13","host_type":"publisher"},{"url":"https://doi.org/10.1128/aem.03016-13","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24212576","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3911107","host_type":"repository"}],"fields_of_study":["Gut microbiota and health","Probiotics and Fermented Foods","Ruminant Nutrition and Digestive Physiology","Medicine","Biology","Environmental Science","Animals","Bacteria","Bacteriological Techniques","Bacteroidetes","Biodiversity","Cattle","Cellulose","Feces","Female","Molecular Sequence Data","Phylogeny","Polysaccharides","RNA, Ribosomal, 16S","Xylans"],"mesh_terms":["Animals","Bacteria","Bacteriological Techniques","Cattle","Cellulose","Feces","Female","Molecular Sequence Data","Phylogeny","Polysaccharides","RNA, Ribosomal, 16S","Xylans","Bacteroidetes","Biodiversity"],"keywords":["Firmicutes","Lachnospiraceae","Bacteroidetes","Biology","Ruminococcus","Proteobacteria","Microbiology","Bacteroides","Bacteria","Actinobacteria","Bacteroides thetaiotaomicron","Fusobacteria","Bacteroidaceae","Microbiome","Clostridium","Feces","16S ribosomal RNA","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.14759907.v1","title":"Additional file 1 of Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14759907","title":"Additional file 1 of Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14759910.v1","title":"Additional file 2 of Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14759910","title":"Additional file 2 of Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14759913.v1","title":"Additional file 3 of Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14759913","title":"Additional file 3 of Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T16:54:18.104167Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}