{"doi":"10.1128/aem.02760-07","title":"Dynamics of\n            <i>Legionella</i>\n            spp. and Bacterial Populations during the Proliferation of\n            <i>L. pneumophila</i>\n            in a Cooling Tower Facility","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The dynamics of\n            <jats:italic>Legionella</jats:italic>\n            spp. and of dominant bacteria were investigated in water from a cooling tower plant over a 9-month period which included several weeks when\n            <jats:italic>Legionella pneumophila</jats:italic>\n            proliferated. The structural diversity of both the bacteria and the\n            <jats:italic>Legionella</jats:italic>\n            spp. was monitored by a fingerprint technique, single-strand conformation polymorphism, and\n            <jats:italic>Legionella</jats:italic>\n            spp. and\n            <jats:italic>L. pneumophila</jats:italic>\n            were quantified by real-time quantitative PCR. The structure of the bacterial community did not change over time, but it was perturbed periodically by chemical treatment or biofilm detachment. In contrast, the structure of the\n            <jats:italic>Legionella</jats:italic>\n            sp. population changed in different periods, its dynamics at times showing stability but also a rapid major shift during the proliferation of\n            <jats:italic>L. pneumophila</jats:italic>\n            in July. The dynamics of the\n            <jats:italic>Legionella</jats:italic>\n            spp. and of dominant bacteria were not correlated. In particular, no change in the bacterial community structure was observed during the proliferation of\n            <jats:italic>L. pneumophila. Legionella</jats:italic>\n            spp. present in the cooling tower system were identified by cloning and sequencing of 16S rRNA genes. A high diversity of\n            <jats:italic>Legionella</jats:italic>\n            spp. was observed before proliferation, including\n            <jats:italic>L. lytica, L. fallonii</jats:italic>\n            , and other\n            <jats:italic>Legionella</jats:italic>\n            -like amoebal pathogen types, along with as-yet-undescribed species. During the proliferation of\n            <jats:italic>L. pneumophila, Legionella</jats:italic>\n            sp. diversity decreased significantly,\n            <jats:italic>L. fallonii</jats:italic>\n            and\n            <jats:italic>L. pneumophila</jats:italic>\n            being the main species recovered.\n          </jats:p>","journal":"Applied and Environmental Microbiology","year":2008,"id":597543,"datarank":0.7023196840686331,"base_score":4.68213122712422,"endowment":4.68213122712422,"self_citation_contribution":0.7023196840686331,"citation_network_contribution":0.0,"self_endowment_contribution":0.7023196840686331,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":107,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1530868,"name":"Valérie Bru-Adan","orcid":null,"position":1,"is_corresponding":false},{"id":1530869,"name":"Céline Minervini","orcid":null,"position":2,"is_corresponding":false},{"id":1530870,"name":"Jean-Philippe Delgénes","orcid":null,"position":3,"is_corresponding":false},{"id":1530871,"name":"Laurent Garrelly","orcid":null,"position":4,"is_corresponding":false},{"id":1530872,"name":"Jean-Jacques Godon","orcid":null,"position":5,"is_corresponding":false},{"id":1530866,"name":"Nathalie Wéry","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dynamics of\n            <i>Legionella</i>\n            spp. and Bacterial Populations during the Proliferation of\n            <i>L. pneumophila</i>\n            in a Cooling Tower Facility","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The dynamics of\n            <jats:italic>Legionella</jats:italic>\n            spp. and of dominant bacteria were investigated in water from a cooling tower plant over a 9-month period which included several weeks when\n            <jats:italic>Legionella pneumophila</jats:italic>\n            proliferated. The structural diversity of both the bacteria and the\n            <jats:italic>Legionella</jats:italic>\n            spp. was monitored by a fingerprint technique, single-strand conformation polymorphism, and\n            <jats:italic>Legionella</jats:italic>\n            spp. and\n            <jats:italic>L. pneumophila</jats:italic>\n            were quantified by real-time quantitative PCR. The structure of the bacterial community did not change over time, but it was perturbed periodically by chemical treatment or biofilm detachment. In contrast, the structure of the\n            <jats:italic>Legionella</jats:italic>\n            sp. population changed in different periods, its dynamics at times showing stability but also a rapid major shift during the proliferation of\n            <jats:italic>L. pneumophila</jats:italic>\n            in July. The dynamics of the\n            <jats:italic>Legionella</jats:italic>\n            spp. and of dominant bacteria were not correlated. In particular, no change in the bacterial community structure was observed during the proliferation of\n            <jats:italic>L. pneumophila. Legionella</jats:italic>\n            spp. present in the cooling tower system were identified by cloning and sequencing of 16S rRNA genes. A high diversity of\n            <jats:italic>Legionella</jats:italic>\n            spp. was observed before proliferation, including\n            <jats:italic>L. lytica, L. fallonii</jats:italic>\n            , and other\n            <jats:italic>Legionella</jats:italic>\n            -like amoebal pathogen types, along with as-yet-undescribed species. During the proliferation of\n            <jats:italic>L. pneumophila, Legionella</jats:italic>\n            sp. diversity decreased significantly,\n            <jats:italic>L. fallonii</jats:italic>\n            and\n            <jats:italic>L. pneumophila</jats:italic>\n            being the main species recovered.\n          </jats:p>","is_dataset_classified":null,"base_score":4.68213122712422,"endowment":4.68213122712422,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18390683","pmcid":"PMC2394956","openalex_id":"https://openalex.org/W2142946408","authors":[],"funders":[],"total_grants":0,"fwci":5.3893,"citation_percentile":0.96171942,"influential_citations":0,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":9},{"year":2014,"count":5},{"year":2015,"count":6},{"year":2016,"count":6},{"year":2017,"count":9},{"year":2018,"count":4},{"year":2019,"count":7},{"year":2020,"count":8},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":7},{"year":2024,"count":7},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"https://hal.inrae.fr/hal-02666206","host_type":"repository"},{"url":"https://hal.inrae.fr/hal-02666206","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/AEM.02760-07","host_type":"publisher"},{"url":"https://doi.org/10.1128/aem.02760-07","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18390683","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.664.2540","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2394956","host_type":"repository"}],"fields_of_study":["Legionella and Acanthamoeba research","Water Treatment and Disinfection","Bacterial biofilms and quorum sensing"],"mesh_terms":["Amoeba","Animals","Bacteria","DNA, Bacterial","DNA, Ribosomal","Industrial Microbiology","Molecular Sequence Data","Phylogeny","Seasons","Time Factors","Water Microbiology","Polymerase Chain Reaction","DNA Fingerprinting","Sequence Analysis, DNA","Polymorphism, Single-Stranded Conformational","Biodiversity"],"keywords":["Legionella pneumophila","Legionella","Biology","Microbiology","Bacteria","16S ribosomal RNA","Biofilm","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T13:32:59.374934Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}