{"doi":"10.1128/aem.01432-10","title":"Maximizing Capture Efficiency and Specificity of Magnetic Separation for\n            <i>Mycobacterium avium</i>\n            subsp.\n            <i>paratuberculosis</i>\n            Cells","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            In order to introduce specificity for\n            <jats:italic>Mycobacterium avium</jats:italic>\n            subsp.\n            <jats:italic>paratuberculosis</jats:italic>\n            prior to a phage amplification assay, various magnetic-separation approaches, involving either antibodies or peptides, were evaluated in terms of the efficiency of capture (expressed as a percentage) of\n            <jats:italic>M. avium</jats:italic>\n            subsp.\n            <jats:italic>paratuberculosis</jats:italic>\n            cells and the percentage of nonspecific binding by other\n            <jats:italic>Mycobacterium</jats:italic>\n            spp. A 50:50 mixture of MyOne Tosylactivated Dynabeads coated with the chemically synthesized\n            <jats:italic>M. avium</jats:italic>\n            subsp.\n            <jats:italic>paratuberculosis</jats:italic>\n            -specific peptides biotinylated aMp3 and biotinylated aMptD (i.e., peptide-mediated magnetic separation [PMS]) proved to be the best magnetic-separation approach for achieving 85 to 100% capture of\n            <jats:italic>M. avium</jats:italic>\n            subsp.\n            <jats:italic>paratuberculosis</jats:italic>\n            and minimal (&lt;1%) nonspecific recovery of other\n            <jats:italic>Mycobacterium</jats:italic>\n            spp. (particularly if beads were blocked with 1% skim milk before use) from broth samples containing 10\n            <jats:sup>3</jats:sup>\n            to 10\n            <jats:sup>4</jats:sup>\n            CFU/ml. When PMS was coupled with a recently optimized phage amplification assay and used to detect\n            <jats:italic>M. avium</jats:italic>\n            subsp.\n            <jats:italic>paratuberculosis</jats:italic>\n            in 50-ml volumes of spiked milk, the mean 50% limit of detection (LOD\n            <jats:sub>50</jats:sub>\n            ) was 14.4 PFU/50 ml of milk (equivalent to 0.3 PFU/ml). This PMS-phage assay represents a novel, rapid method for the detection and enumeration of viable\n            <jats:italic>M. avium</jats:italic>\n            subsp.\n            <jats:italic>paratuberculosis</jats:italic>\n            organisms in milk, and potentially other sample matrices, with results available within 48 h.\n          </jats:p>","journal":"Applied and Environmental Microbiology","year":2010,"id":623338,"datarank":0.6646225198264971,"base_score":4.430816798843313,"endowment":4.430816798843313,"self_citation_contribution":0.6646225198264971,"citation_network_contribution":0.0,"self_endowment_contribution":0.6646225198264971,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":83,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":655579,"name":"Christopher T. Elliott","orcid":"0000-0003-0495-2909","position":1,"is_corresponding":false},{"id":1610893,"name":"Irene R. Grant","orcid":null,"position":2,"is_corresponding":false},{"id":1610892,"name":"Antonio Foddai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-08-03T23:21:08.551751Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}