{"doi":"10.1128/aem.01330-08","title":"Effective Mutagenesis of\n            <i>Vibrio fischeri</i>\n            by Using Hyperactive Mini-Tn\n            <i>5</i>\n            Derivatives","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            We have developed a transposon mutagenesis system for\n            <jats:italic>Vibrio fischeri</jats:italic>\n            ES114 that utilizes a hyperactive mutant Tn\n            <jats:italic>5</jats:italic>\n            transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn\n            <jats:italic>5</jats:italic>\n            mutants at a rate of ∼10\n            <jats:sup>−6</jats:sup>\n            . This simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of\n            <jats:italic>V. fischeri</jats:italic>\n            and should be applicable to many other bacteria.\n          </jats:p>","journal":"Applied and Environmental Microbiology","year":2008,"id":686463,"datarank":0.5641800173540344,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"self_citation_contribution":0.5641800173540344,"citation_network_contribution":0.0,"self_endowment_contribution":0.5641800173540344,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1793387,"name":"Anne K. Dunn","orcid":null,"position":1,"is_corresponding":false},{"id":314722,"name":"Jeffrey L. Bose","orcid":"0000-0003-3394-9557","position":2,"is_corresponding":false},{"id":1793388,"name":"Susan L. Vescovi","orcid":null,"position":3,"is_corresponding":false},{"id":628730,"name":"Eric V. Stabb","orcid":"0000-0001-7000-4275","position":4,"is_corresponding":false},{"id":1793385,"name":"Noreen L. Lyell","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Effective Mutagenesis of\n            <i>Vibrio fischeri</i>\n            by Using Hyperactive Mini-Tn\n            <i>5</i>\n            Derivatives","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            We have developed a transposon mutagenesis system for\n            <jats:italic>Vibrio fischeri</jats:italic>\n            ES114 that utilizes a hyperactive mutant Tn\n            <jats:italic>5</jats:italic>\n            transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn\n            <jats:italic>5</jats:italic>\n            mutants at a rate of ∼10\n            <jats:sup>−6</jats:sup>\n            . This simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of\n            <jats:italic>V. fischeri</jats:italic>\n            and should be applicable to many other bacteria.\n          </jats:p>","is_dataset_classified":null,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18805998","pmcid":"PMC2583470","openalex_id":"https://openalex.org/W2121868431","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"AI50661","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI050661","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R37 AI050661","title":null}],"total_grants":3,"fwci":1.0843,"citation_percentile":0.75329609,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":4},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":6},{"year":2019,"count":2},{"year":2020,"count":6},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2024,"count":3},{"year":2025,"count":2}],"oa_status":"closed","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://journals.asm.org/doi/pdf/10.1128/AEM.01330-08","host_type":"publisher"},{"url":"https://doi.org/10.1128/aem.01330-08","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18805998","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2583470","host_type":"repository"}],"fields_of_study":["Vibrio bacteria research studies","Advanced biosensing and bioanalysis techniques","Aquaculture disease management and microbiota","Aliivibrio fischeri","Blotting, Southern","DNA Transposable Elements","DNA, Bacterial","Mutagenesis, Insertional"],"mesh_terms":["DNA Transposable Elements","DNA, Bacterial","Blotting, Southern","Mutagenesis, Insertional","Aliivibrio fischeri"],"keywords":["Transposable element","Transposon mutagenesis","Mutagenesis","Vibrio","Transposase","Mutant","Biology","Genetics","Sleeping Beauty transposon system","Bacteria","P element","DNA Transposable Elements","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T19:33:55.911533Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}