{"doi":"10.1128/aem.00358-22","title":"Regiospecific Oxidation of Chlorobenzene to 4-Chlororesorcinol, Chlorohydroquinone, 3-Chlorocatechol and 4-Chlorocatechol by Engineered Toluene <i>o</i> -Xylene Monooxygenases","abstract":"-xylene monooxygenase (ToMO) can successfully oxidize chlorobenzene to form dihydroxy chloroaromatics, which are valuable industrial compounds. ToMO performs this at room temperature in water using only molecular oxygen and a cofactor supplied by the cells. Using protein engineering techniques, we also isolated ToMO variants with enhanced oxidation activity as well as fine-tuned regiospecificities which make direct microbial oxygenations even more attractive. The significance of this work lies in the ability to degrade environmental pollutants while at the same time producing valuable chemicals using environmentally benign biological methods rather than expensive, complex chemical processes.","journal":"Applied and Environmental Microbiology","year":2022,"id":291122,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":974394,"name":"Onkar K. Phul","orcid":null,"position":1,"is_corresponding":false},{"id":973992,"name":"Owen S. Roth","orcid":"0000-0002-2770-5970","position":2,"is_corresponding":false},{"id":974395,"name":"Areli Tlatelpa","orcid":null,"position":3,"is_corresponding":false},{"id":974396,"name":"Gerardo Soria-P","orcid":null,"position":4,"is_corresponding":false},{"id":973993,"name":"Nurcan Vardar-Yel","orcid":"0000-0003-0994-5871","position":5,"is_corresponding":false},{"id":973994,"name":"Gönül Vardar‐Schara","orcid":"0000-0002-0858-3170","position":6,"is_corresponding":false},{"id":974393,"name":"K. Cansu Yanık‐Yıldırım","orcid":null,"position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-19T00:30:34.502076Z","pmid":"35736230","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}