{"doi":"10.1128/aem.00335-23","title":"Export of Diverse and Bioactive Small Proteins through a Type I Secretion System","abstract":"Type I secretion systems for microcin export in Gram-negative bacteria transport small antibacterial proteins from the cytoplasm to the extracellular environment in a single step. In nature, each secretion system is generally paired with a specific small protein. We know little about the export capacity of these transporters and how cargo sequence influences secretion. Here, we investigate the microcin V type I system. Remarkably, our studies show that this system can export small proteins of diverse sequence composition and is only limited by protein length. Furthermore, we demonstrate that a wide range of bioactive small proteins can be secreted and that this system can be used in Gram-negative species that colonize the gastrointestinal tract. These findings expand our understanding of secretion through type I systems and their potential uses in a variety of small-protein applications.","journal":"Applied and Environmental Microbiology","year":2023,"id":340985,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":498040,"name":"Jennifer K. Parker","orcid":"0000-0002-4382-0741","position":1,"is_corresponding":false},{"id":581611,"name":"Mónica González-Magaldi","orcid":"0000-0001-9022-4590","position":2,"is_corresponding":false},{"id":1076083,"name":"Mady S. Telford","orcid":"0000-0002-8799-4743","position":3,"is_corresponding":false},{"id":495975,"name":"Daniel J. Leahy","orcid":"0000-0001-7074-2358","position":4,"is_corresponding":false},{"id":325688,"name":"Bryan W. Davies","orcid":"0000-0002-2046-0341","position":5,"is_corresponding":false},{"id":1076082,"name":"Sun‐Young Kim","orcid":"0000-0003-1333-6147","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-19T01:10:58.548803Z","pmid":"37078870","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}