{"doi":"10.1128/aem.00250-20","title":"Xylose-Inducible Promoter Tools for <i>Pseudomonas</i> Species and Their Use in Implicating a Role for the Type II Secretion System Protein XcpQ in the Inhibition of Corneal Epithelial Wound Closure","abstract":"Pseudomonas species are enormously important in human infections, in biotechnology, and as model systems for investigating basic science questions. In this study, we have developed a xylose-inducible promoter system, evaluated it in P. aeruginosa and P. fluorescens , and found it to be suitable for the strong induction of gene expression. Furthermore, we have demonstrated its efficacy in controlled gene expression to show that a type II secretion system protein from P. aeruginosa , XcpQ, is important for host-pathogen interactions in a corneal wound closure model.","journal":"Applied and Environmental Microbiology","year":2020,"id":89025,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":450899,"name":"Nicholas A. Stella","orcid":"0000-0002-6591-6912","position":1,"is_corresponding":false},{"id":452033,"name":"Kara M. Lehner","orcid":null,"position":2,"is_corresponding":false},{"id":450900,"name":"Benjamin R. Treat","orcid":"0000-0002-3385-0250","position":3,"is_corresponding":false},{"id":450901,"name":"Kimberly M. Brothers","orcid":"0000-0003-2055-9760","position":4,"is_corresponding":false},{"id":325806,"name":"Anthony J. St. Leger","orcid":"0000-0002-2547-200X","position":5,"is_corresponding":false},{"id":425714,"name":"Robert M. Q. Shanks","orcid":"0000-0002-7699-5396","position":6,"is_corresponding":false},{"id":450898,"name":"Jake D. Callaghan","orcid":"0000-0002-1155-0855","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-18T22:01:50.225871Z","pmid":"32414795","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}