{"doi":"10.1128/aem.00172-09","title":"Bile-Inducible Efflux Transporter from\n            <i>Bifidobacterium longum</i>\n            NCC2705, Conferring Bile Resistance","abstract":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Bifidobacteria are normal inhabitants of the human gut. Some strains of this genus are considered health promoting or probiotic, being included in numerous food products. In order to exert their health benefits, these bacteria must overcome biological barriers, including bile salts, to colonize and survive in specific parts of the intestinal tract. The role of multidrug resistance (MDR) transporters in bile resistance of probiotic bacteria and the effect of bile on probiotic gene expression are not fully understood. In the present study, the effect of subinhibitory concentrations of bile on the expression levels of predicted MDR genes from three different bifidobacterial strains, belonging to\n            <jats:italic>Bifidobacterium longum</jats:italic>\n            subsp.\n            <jats:italic>longum, Bifidobacterium breve</jats:italic>\n            , and\n            <jats:italic>Bifidobacterium animalis</jats:italic>\n            subsp.\n            <jats:italic>lactis</jats:italic>\n            , was tested. In this way, two putative MDR genes whose expression was induced by bile, BL0920 from\n            <jats:italic>B. longum</jats:italic>\n            and its homolog, Bbr0838, from\n            <jats:italic>B. breve</jats:italic>\n            , were identified. The expression of the BL0920 gene in\n            <jats:italic>Escherichia coli</jats:italic>\n            was shown to confer resistance to bile, likely to be mediated by active efflux from the cells. To the best of our knowledge, this represents the first identified bifidobacterial bile efflux pump whose expression is induced by bile.\n          </jats:p>","journal":"Applied and Environmental Microbiology","year":2009,"id":27828,"datarank":2.6557026036929137,"base_score":4.394449154672439,"endowment":4.394449154672439,"self_citation_contribution":0.6591673732008659,"citation_network_contribution":1.9965352304920476,"self_endowment_contribution":0.6591673732008659,"citer_contribution":1.9965352304920476,"corpus_percentile":null,"corpus_rank":null,"citation_count":80,"citer_count":61,"citers_with_citation_signal":49,"citers_with_endowment":49,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":157507,"name":"Christel Garrigues","orcid":null,"position":1,"is_corresponding":false},{"id":124924,"name":"Douwe van Sinderen","orcid":null,"position":2,"is_corresponding":false},{"id":157508,"name":"Clara G. de los Reyes-Gavilán","orcid":null,"position":3,"is_corresponding":false},{"id":157509,"name":"Abelardo Margolles","orcid":null,"position":4,"is_corresponding":false},{"id":140496,"name":"Miguel Gueimonde","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.394449154672439,"endowment":4.394449154672439,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19304838","pmcid":"PMC2681658","openalex_id":"https://openalex.org/W2120137398","authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"CC2003","title":null}],"total_grants":1,"fwci":11.3544,"citation_percentile":0.98549877,"influential_citations":3,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":4},{"year":2014,"count":8},{"year":2015,"count":1},{"year":2016,"count":4},{"year":2017,"count":5},{"year":2018,"count":9},{"year":2019,"count":2},{"year":2020,"count":5},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":3},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2681658","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc2681658?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2681658","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/AEM.00172-09","host_type":"publisher"},{"url":"https://doi.org/10.1128/aem.00172-09","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19304838","host_type":"repository"}],"fields_of_study":["Probiotics and Fermented Foods","Antibiotic Resistance in Bacteria","Drug Transport and Resistance Mechanisms","Medicine","Biology","Environmental Science","Anti-Bacterial Agents","Bacterial Proteins","Bifidobacterium","Bile","DNA, Bacterial","Drug Resistance, Bacterial","Escherichia coli","Gene Expression Profiling","Membrane Transport Proteins","Molecular Sequence Data","Phylogeny","Sequence Analysis, DNA","Sequence Homology"],"mesh_terms":["Anti-Bacterial Agents","Bacterial Proteins","Bifidobacterium","Bile","DNA, Bacterial","Escherichia coli","Molecular Sequence Data","Phylogeny","Sequence Homology","Sequence Analysis, DNA","Gene Expression Profiling","Drug Resistance, Bacterial","Membrane Transport Proteins"],"keywords":["Bifidobacterium longum","Bifidobacterium breve","Efflux","Probiotic","Bifidobacterium animalis","Biology","Microbiology","Bifidobacterium","Bacteria","Human gastrointestinal tract","ATP-binding cassette transporter","Gene","Transporter","Lactobacillus","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"pfam"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T19:34:28.784474Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}