{"doi":"10.1128/aac.29.6.1053","title":"Characterization of a varicella-zoster virus variant with altered thymidine kinase activity","abstract":"<jats:p>A varicella-zoster virus (VZV) strain resistant to 5-iodo-2'-deoxyuridine (IdUrd) and 5-bromo-2'-deoxyuridine (BrdUrd) but sensitive to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdUrd) and (E)-5-(2-iodovinyl)-2'-deoxyuridine (IVdUrd) was isolated. The 2'-deoxythymidine (dThd) kinase of this mutant (Ito) strain was characterized; it was much less efficient in phosphorylating dThd, 2'-deoxycytidine, and BrdUrd than were the dThd kinases from wild-type (CaQu, Kobayashi) VZV strains. The Ito dThd kinase had a markedly decreased affinity for dThd, 2'-deoxycytidine, and BrdUrd but only a slightly decreased affinity for IVdUrd than had the wild-type VZV dThd kinase. BrdUrd was incorporated to a much lesser extent in VZV (Ito strain)-infected cells than wild-type VZV-infected cells, but IVdUrd was incorporated in Ito VZV-infected cells as efficiently as in wild-type VZV-infected cells. While resistant to IdUrd and BrdUrd, the Ito strain was susceptible to inhibitors of de novo thymidylate biosynthesis such as aminopterin.</jats:p>","journal":"Antimicrobial Agents and Chemotherapy","year":1986,"id":614438,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1583307,"name":"S Mori","orcid":null,"position":1,"is_corresponding":false},{"id":147204,"name":"T Yokota","orcid":null,"position":2,"is_corresponding":false},{"id":1583308,"name":"K Konno","orcid":null,"position":3,"is_corresponding":false},{"id":1583309,"name":"E De Clercq","orcid":null,"position":4,"is_corresponding":false},{"id":1583306,"name":"S Shigeta","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Characterization of a varicella-zoster virus variant with altered thymidine kinase activity","abstract":"<jats:p>A varicella-zoster virus (VZV) strain resistant to 5-iodo-2'-deoxyuridine (IdUrd) and 5-bromo-2'-deoxyuridine (BrdUrd) but sensitive to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdUrd) and (E)-5-(2-iodovinyl)-2'-deoxyuridine (IVdUrd) was isolated. The 2'-deoxythymidine (dThd) kinase of this mutant (Ito) strain was characterized; it was much less efficient in phosphorylating dThd, 2'-deoxycytidine, and BrdUrd than were the dThd kinases from wild-type (CaQu, Kobayashi) VZV strains. The Ito dThd kinase had a markedly decreased affinity for dThd, 2'-deoxycytidine, and BrdUrd but only a slightly decreased affinity for IVdUrd than had the wild-type VZV dThd kinase. BrdUrd was incorporated to a much lesser extent in VZV (Ito strain)-infected cells than wild-type VZV-infected cells, but IVdUrd was incorporated in Ito VZV-infected cells as efficiently as in wild-type VZV-infected cells. While resistant to IdUrd and BrdUrd, the Ito strain was susceptible to inhibitors of de novo thymidylate biosynthesis such as aminopterin.</jats:p>","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"3015014","pmcid":"PMC180499","openalex_id":"https://openalex.org/W2095124314","authors":[],"funders":[],"total_grants":0,"fwci":1.3019,"citation_percentile":0.81392307,"influential_citations":0,"citation_trend":[{"year":2020,"count":1}],"oa_status":"closed","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://journals.asm.org/doi/pdf/10.1128/AAC.29.6.1053","host_type":"publisher"},{"url":"https://doi.org/10.1128/aac.29.6.1053","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/3015014","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC180499","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/180499","host_type":"repository"}],"fields_of_study":["Herpesvirus Infections and Treatments","melanin and skin pigmentation","Pharmacological Effects of Natural Compounds","Aminopterin","Antiviral Agents","Bromodeoxyuridine","Cells, Cultured","Chromatography, Affinity","Electrophoresis, Polyacrylamide Gel","Floxuridine","Herpesvirus 3, Human","Humans","Idoxuridine","Microbial Sensitivity Tests","Phosphorylation","Thymidine","Thymidine Kinase"],"mesh_terms":["Aminopterin","Antiviral Agents","Bromodeoxyuridine","Cells, Cultured","Chromatography, Affinity","Electrophoresis, Polyacrylamide Gel","Floxuridine","Humans","Idoxuridine","Microbial Sensitivity Tests","Phosphorylation","Thymidine","Thymidine Kinase","Herpesvirus 3, Human"],"keywords":["Thymidine kinase","Deoxyuridine","Idoxuridine","Thymidine","Deoxycytidine kinase","Varicella zoster virus","Bromodeoxyuridine","Kinase","Biology","Virology","Molecular biology","Virus","Biochemistry","Deoxycytidine","Chemistry","In vitro","DNA","Herpes simplex virus","Cell growth","Chemotherapy","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T13:27:21.507549Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}