{"doi":"10.1128/aac.01806-24","title":"Frequencies of molecular markers of drug resistance in the context of two different Seasonal Malaria Chemoprevention (SMC) treatment regimens in the Koulikoro health district, Mali","abstract":"ABSTRACT With growing concern about parasite resistance to sulfadoxine-pyrimethamine (SP) in West Africa, the effectiveness of dihydroartemisinin-piperaquine (DHA + PQ) was assessed as an alternative drug regimen for Seasonal Malaria Chemoprevention (SMC). This study aims to determine the prevalence of molecular markers of resistance to SP + AQ and DHA + PQ in Koulikoro (Mali), where SMC has been implemented since 2014. Plasmodium falciparum -positive samples were analyzed by either next-generation sequencing, focusing on SNPs in genes known to be associated with resistance: Pfmdr1, PfK13, Pfcrt, Pfdhfr, Pfdhps, and Pfexo genes, and using qPCR for copy number variations (CNVs) of Pfmdr1 and Pfplasmepsin2 . A total of 564 PCR-positive P. falciparum samples were analyzed: 218 in 2019 and 346 in 2020. In both years, the Pfdhfr 51I-59R-108N haplotype was highly prevalent (>93%). In both arms, the Pfdhps single mutant ( 437G ) was the most prevalent (~60%). In 2020, the combined haplotype Pfdhfr/Pfdhps IRN/ IA G KA S and IRN/V A G K GS was detected at 9.6% and 1.2%, respectively. The Pfmdr1 haplotype (N86- F184 -S1034-N1042-D1246) represented more than 52.0%, and no difference was observed between the years, while a significant increase in the Pfcrt wild-type haplotype (C72-V73-M74-N75-K76) from 39% in 2019 to 52% in 2020 ( P = 0.01) was observed. Some PfK13 non-synonymous mutations were observed in both years, including Y 493H and C 580R . The identification of the IRN I / IA G KA S quintuple mutant, along with emerging Pfdhps -431V and non-synonymous PfK13- Y493H variants, underscores the importance of continued surveillance of resistance markers.","journal":"Antimicrobial Agents and Chemotherapy","year":2025,"id":572593,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9512,"is_data_producer":true,"deposit_databanks":{"BioProject":["PRJNA1299543"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":467260,"name":"Fousseyni Kané","orcid":"0000-0002-9967-7956","position":1,"is_corresponding":false},{"id":403036,"name":"Mahamoudou Touré","orcid":"0000-0001-6238-5063","position":2,"is_corresponding":false},{"id":1062181,"name":"Marie Helene Munck Jørgensen","orcid":null,"position":3,"is_corresponding":false},{"id":427714,"name":"Daouda Sanogo","orcid":"0009-0007-1152-5231","position":4,"is_corresponding":false},{"id":948117,"name":"Soumba Keita","orcid":"0009-0006-3248-4961","position":5,"is_corresponding":false},{"id":334201,"name":"Moussa Keïta","orcid":"0009-0009-6396-3801","position":6,"is_corresponding":false},{"id":403035,"name":"Nafomon Sogoba","orcid":"0000-0003-2926-220X","position":7,"is_corresponding":false},{"id":242114,"name":"Kathryn Shaw‐Saliba","orcid":"0000-0002-4396-2432","position":8,"is_corresponding":false},{"id":384668,"name":"Mahamadou Diakité","orcid":"0000-0002-4268-8857","position":9,"is_corresponding":false},{"id":330630,"name":"Jeffrey G. Shaffer","orcid":"0000-0002-3941-3772","position":10,"is_corresponding":false},{"id":940302,"name":"Michael Alifrangis","orcid":"0000-0002-3835-3333","position":11,"is_corresponding":false},{"id":1061558,"name":"Helle Hansson","orcid":"0000-0001-6484-1165","position":12,"is_corresponding":false},{"id":262078,"name":"Seydou Doumbia","orcid":"0000-0003-1637-5600","position":13,"is_corresponding":false},{"id":797963,"name":"Bourama Traoré","orcid":"0000-0002-4967-5987","position":0,"is_corresponding":true}],"reference_count":60,"raw_metadata":null,"created_at":"2026-07-19T02:57:27.876396Z","pmid":"40823868","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}