{"doi":"10.1128/aac.00893-07","title":"Impact of Nucleoside Reverse Transcriptase Inhibitors on Mitochondria in Human Immunodeficiency Virus Type 1-Infected Children Receiving Highly Active Antiretroviral Therapy","abstract":"<jats:title>ABSTRACT</jats:title><jats:p>Mitochondrial toxicity induced by nucleoside reverse transcriptase inhibitors (NRTIs) has been reported to be responsible for various adverse effects. The relative impact of NRTIs on the mitochondria of human immunodeficiency virus (HIV) type 1 (HIV-1)-infected children receiving highly active antiretroviral therapy (HAART) is unknown. Mitochondrial DNA (mtDNA) levels were quantified longitudinally from peripheral blood mononuclear cells (PBMCs) in 31 HIV-1-infected children from Pediatric AIDS Clinical Trial Group Study 382 who were receiving HAART, including nelfinavir, efavirenz, and different NRTIs, and who had had undetectable plasma HIV-1 RNA levels for &gt;2 years. The median mtDNA levels in PBMCs increased from 137 copies/cell at the baseline to 179 copies/cell at week 48 (<jats:italic>P</jats:italic>= 0.01) and 198 copies/cell at week 104 (<jats:italic>P</jats:italic>&lt; 0.001). Before the initiation of HAART, children who received regimens containing didanosine had mtDNA levels persistently lower than those in children not receiving didanosine (106 versus 140 copies/cell;<jats:italic>P</jats:italic>= 0.008). During HAART, the median increase in the mtDNA level from the baseline to week 104 was the lowest in children who received regimens containing didanosine (+26 copies/cell) compared to those in children who received other regimens (+79 copies/cell) (<jats:italic>P</jats:italic>= 0.02). A multivariate analysis also demonstrated that didanosine, as part of HAART, was the only NRTI associated with the change in mtDNA levels (<jats:italic>P</jats:italic>= 0.007). Children receiving didanosine-containing antiretroviral regimens have the lowest mtDNA levels in PBMCs and may be at greater risk for long-term adverse effects due to mitochondrial toxicity. This may be of particular importance in resource-limited countries where didanosine is widely used for the treatment of HIV-infected children.</jats:p>","journal":"Antimicrobial Agents and Chemotherapy","year":2007,"id":619302,"datarank":0.5897738449086489,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"self_citation_contribution":0.5897738449086489,"citation_network_contribution":0.0,"self_endowment_contribution":0.5897738449086489,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":943906,"name":"Terence Fenton","orcid":null,"position":1,"is_corresponding":false},{"id":1141747,"name":"Carmelita Alvero","orcid":null,"position":2,"is_corresponding":false},{"id":259563,"name":"Courtney V. Fletcher","orcid":"0000-0002-3703-7849","position":3,"is_corresponding":false},{"id":69476,"name":"Stephen A. Spector","orcid":"0000-0002-1882-4855","position":4,"is_corresponding":false},{"id":1598131,"name":"Akihiko Saitoh","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Impact of Nucleoside Reverse Transcriptase Inhibitors on Mitochondria in Human Immunodeficiency Virus Type 1-Infected Children Receiving Highly Active Antiretroviral Therapy","abstract":"<jats:title>ABSTRACT</jats:title><jats:p>Mitochondrial toxicity induced by nucleoside reverse transcriptase inhibitors (NRTIs) has been reported to be responsible for various adverse effects. The relative impact of NRTIs on the mitochondria of human immunodeficiency virus (HIV) type 1 (HIV-1)-infected children receiving highly active antiretroviral therapy (HAART) is unknown. Mitochondrial DNA (mtDNA) levels were quantified longitudinally from peripheral blood mononuclear cells (PBMCs) in 31 HIV-1-infected children from Pediatric AIDS Clinical Trial Group Study 382 who were receiving HAART, including nelfinavir, efavirenz, and different NRTIs, and who had had undetectable plasma HIV-1 RNA levels for &gt;2 years. The median mtDNA levels in PBMCs increased from 137 copies/cell at the baseline to 179 copies/cell at week 48 (<jats:italic>P</jats:italic>= 0.01) and 198 copies/cell at week 104 (<jats:italic>P</jats:italic>&lt; 0.001). Before the initiation of HAART, children who received regimens containing didanosine had mtDNA levels persistently lower than those in children not receiving didanosine (106 versus 140 copies/cell;<jats:italic>P</jats:italic>= 0.008). During HAART, the median increase in the mtDNA level from the baseline to week 104 was the lowest in children who received regimens containing didanosine (+26 copies/cell) compared to those in children who received other regimens (+79 copies/cell) (<jats:italic>P</jats:italic>= 0.02). A multivariate analysis also demonstrated that didanosine, as part of HAART, was the only NRTI associated with the change in mtDNA levels (<jats:italic>P</jats:italic>= 0.007). Children receiving didanosine-containing antiretroviral regimens have the lowest mtDNA levels in PBMCs and may be at greater risk for long-term adverse effects due to mitochondrial toxicity. This may be of particular importance in resource-limited countries where didanosine is widely used for the treatment of HIV-infected children.</jats:p>","is_dataset_classified":null,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17893156","pmcid":"PMC2167993","openalex_id":"https://openalex.org/W2111018497","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"AI-36214","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"5K23AI-56931","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"UM1 AI068632","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI068632","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"P30 AI036214","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"U01 AI068632","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"K23 AI056931","title":null}],"total_grants":7,"fwci":2.485,"citation_percentile":0.8889213,"influential_citations":0,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":6},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":2},{"year":2017,"count":2},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2024,"count":1},{"year":2025,"count":3}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2167993","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2167993","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/AAC.00893-07","host_type":"publisher"},{"url":"https://doi.org/10.1128/aac.00893-07","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17893156","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.321.8539","host_type":""}],"fields_of_study":["HIV/AIDS drug development and treatment","HIV-related health complications and treatments","HIV Research and Treatment"],"mesh_terms":["Adolescent","Age Factors","Alkynes","Body Weight","Child","Child, Preschool","Cyclopropanes","DNA, Mitochondrial","DNA, Viral","Humans","Leukocytes, Mononuclear","Lipids","Longitudinal Studies","Mitochondria","RNA, Viral","HIV-1","HIV Infections","Body Mass Index","Polymerase Chain Reaction","CD4 Lymphocyte Count","Reverse Transcriptase Inhibitors","Nelfinavir","Antiretroviral Therapy, Highly Active","Benzoxazines"],"keywords":["Didanosine","Efavirenz","Mitochondrial toxicity","Peripheral blood mononuclear cell","Reverse-transcriptase inhibitor","Nucleoside Reverse Transcriptase Inhibitor","Nelfinavir","Virology","Adverse effect","Nevirapine","Stavudine","Reverse transcriptase","Medicine","Biology","Immunology","Viral load","Internal medicine","Viral disease","Zidovudine","Virus","Human immunodeficiency virus (HIV)","Antiretroviral therapy","Polymerase chain reaction","In vitro"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T06:41:54.240474Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}