{"doi":"10.1128/aac.00692-10","title":"Environmental Study of Azole-Resistant\n Aspergillus fumigatus\n and Other Aspergilli in Austria, Denmark, and Spain","abstract":"ABSTRACT\n \n A single mechanism of azole resistance was shown to predominate in clinical and environmental\n Aspergillus fumigatus\n isolates from the Netherlands, and a link to the use of azoles in the environment was suggested. To explore the prevalence of azole-resistant\n A. fumigatus\n and other aspergilli in the environment in other European countries, we collected samples from the surroundings of hospitals in Copenhagen, Innsbruck, and Madrid, flowerbeds in an amusement park in Copenhagen, and compost bags purchased in Austria, Denmark, and Spain and screened for azole resistance using multidish agars with itraconazole, voriconazole, and posaconazole. EUCAST method E.DEF 9.1 was used to confirm azole resistance. The promoter and entire coding sequence of the\n cyp51A\n gene were sequenced to identify azole-resistant\n A. fumigatus\n isolates.\n A. fumigatus\n was recovered in 144 out of 185 samples (77.8%). Four\n A. fumigatus\n isolates from four Danish soil samples displayed elevated azole MICs (8%), and all harbored the same TR/L98H mutation of\n cyp51A\n . One\n A. lentulus\n isolate with voriconazole MIC of 4 mg/liter was detected in Spain. No azole-resistant aspergilli were detected in compost. Finally,\n A. terreus\n was present in seven samples from Austria. Multi-azole-resistant\n A. fumigatus\n is present in the environment in Denmark. The resistance mechanism is identical to that of environmental isolates in the Netherlands. No link to commercial compost could be detected. In Spain and Austria, only\n Aspergillus\n species with intrinsic resistance to either azoles or amphotericin B were found.\n ","journal":"Antimicrobial Agents and Chemotherapy","year":2010,"id":646665,"datarank":0.8195747707538417,"base_score":5.4638318050256105,"endowment":5.4638318050256105,"self_citation_contribution":0.8195747707538417,"citation_network_contribution":0.0,"self_endowment_contribution":0.8195747707538417,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":235,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":267292,"name":"Emilia Mellado","orcid":"0000-0002-9801-0260","position":1,"is_corresponding":false},{"id":1684505,"name":"Cornelia Lass-FloĢˆrl","orcid":null,"position":2,"is_corresponding":false},{"id":1602565,"name":"Juan Luis Rodriguez-Tudela","orcid":null,"position":3,"is_corresponding":false},{"id":812643,"name":"Helle Krogh Johansen","orcid":"0000-0003-0268-3717","position":4,"is_corresponding":false},{"id":119981,"name":"Maiken Cavling Arendrup","orcid":"0000-0002-4747-0144","position":5,"is_corresponding":false},{"id":1684504,"name":"Klaus Leth Mortensen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Environmental Study of Azole-Resistant\n Aspergillus fumigatus\n and Other Aspergilli in Austria, Denmark, and Spain","abstract":"ABSTRACT\n \n A single mechanism of azole resistance was shown to predominate in clinical and environmental\n Aspergillus fumigatus\n isolates from the Netherlands, and a link to the use of azoles in the environment was suggested. To explore the prevalence of azole-resistant\n A. fumigatus\n and other aspergilli in the environment in other European countries, we collected samples from the surroundings of hospitals in Copenhagen, Innsbruck, and Madrid, flowerbeds in an amusement park in Copenhagen, and compost bags purchased in Austria, Denmark, and Spain and screened for azole resistance using multidish agars with itraconazole, voriconazole, and posaconazole. EUCAST method E.DEF 9.1 was used to confirm azole resistance. The promoter and entire coding sequence of the\n cyp51A\n gene were sequenced to identify azole-resistant\n A. fumigatus\n isolates.\n A. fumigatus\n was recovered in 144 out of 185 samples (77.8%). Four\n A. fumigatus\n isolates from four Danish soil samples displayed elevated azole MICs (8%), and all harbored the same TR/L98H mutation of\n cyp51A\n . One\n A. lentulus\n isolate with voriconazole MIC of 4 mg/liter was detected in Spain. No azole-resistant aspergilli were detected in compost. Finally,\n A. terreus\n was present in seven samples from Austria. Multi-azole-resistant\n A. fumigatus\n is present in the environment in Denmark. The resistance mechanism is identical to that of environmental isolates in the Netherlands. No link to commercial compost could be detected. In Spain and Austria, only\n Aspergillus\n species with intrinsic resistance to either azoles or amphotericin B were found.\n ","is_dataset_classified":null,"base_score":5.4638318050256105,"endowment":5.4638318050256105,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20805399","pmcid":"PMC2976122","openalex_id":"https://openalex.org/W2057425442","authors":[],"funders":[],"total_grants":0,"fwci":10.3529,"citation_percentile":0.99119187,"influential_citations":0,"citation_trend":[{"year":2012,"count":22},{"year":2013,"count":23},{"year":2014,"count":18},{"year":2015,"count":11},{"year":2016,"count":13},{"year":2017,"count":19},{"year":2018,"count":16},{"year":2019,"count":13},{"year":2020,"count":20},{"year":2021,"count":19},{"year":2022,"count":10},{"year":2023,"count":12},{"year":2024,"count":11},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"https://journals.asm.org/non-commercial-tdm-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2976122","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2976122","host_type":"repository"},{"url":"https://journals.asm.org/doi/pdf/10.1128/AAC.00692-10","host_type":"publisher"},{"url":"https://doi.org/10.1128/aac.00692-10","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20805399","host_type":"repository"},{"url":"https://curis.ku.dk/portal/da/publications/environmental-study-of-azoleresistant-aspergillus-fumigatus-and-other-aspergilli-in-austria-denmark-and-spain(7a1dd5cf-a209-4b80-939e-d1c109613bca).html","host_type":"repository"},{"url":"https://researchprofiles.ku.dk/da/publications/7a1dd5cf-a209-4b80-939e-d1c109613bca","host_type":"repository"}],"fields_of_study":["Antifungal resistance and susceptibility","Fungal Infections and Studies","Plant Pathogens and Fungal Diseases","Antifungal Agents","Aspergillus fumigatus","Austria","Azoles","Denmark","Drug Resistance, Fungal","Environmental Microbiology","Itraconazole","Microbial Sensitivity Tests","Pyrimidines","Spain","Triazoles","Voriconazole"],"mesh_terms":["Antifungal Agents","Aspergillus fumigatus","Austria","Azoles","Denmark","Environmental Microbiology","Microbial Sensitivity Tests","Pyrimidines","Spain","Triazoles","Itraconazole","Drug Resistance, Fungal","Voriconazole"],"keywords":["Aspergillus fumigatus","Azole","Posaconazole","Microbiology","Voriconazole","Biology","Itraconazole","Aspergillus","Aspergillosis","Antifungal","Botany"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T14:22:07.944005Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}