{"doi":"10.1128/aac.00417-23","title":"A truncated HIV Tat demonstrates potent and specific latency reversal activity","abstract":"ABSTRACT A major barrier to HIV-1 cure is caused by the pool of latently infected CD4 T-cells that persist under combination antiretroviral therapy (cART). This latent reservoir is capable of producing replication-competent infectious viruses once prolonged suppressive cART is withdrawn. Inducing the reactivation of HIV-1 gene expression in T-cells harboring a latent provirus in people living with HIV-1 under cART may result in depletion of this latent reservoir due to cytopathic effects or immune clearance. Studies have investigated molecules that reactivate HIV-1 gene expression, but to date, no latency reversal agent has been identified to eliminate latently infected cells harboring replication-competent HIV in cART-treated individuals. Stochastic fluctuations in HIV-1 tat gene expression have been described and hypothesized to allow the progression into proviral latency. We hypothesized that exposing latently infected CD4+ T-cells to Tat would result in effective latency reversal. Our results indicate the capacity of a truncated Tat protein and mRNA to reactivate HIV-1 in latently infected T-cells ex vivo to a similar degree as the protein kinase C agonist: phorbol 12-myristate 13-acetate, without T-cell activation or any significant transcriptome perturbation.","journal":"Antimicrobial Agents and Chemotherapy","year":2023,"id":341788,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9553,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":643960,"name":"Marion Pardons","orcid":"0000-0002-9921-8207","position":1,"is_corresponding":false},{"id":1078327,"name":"Erik Nijs","orcid":null,"position":2,"is_corresponding":false},{"id":1077829,"name":"Nick Verheyen","orcid":"0000-0001-8017-741X","position":3,"is_corresponding":false},{"id":1078328,"name":"Koen Dockx","orcid":null,"position":4,"is_corresponding":false},{"id":1078329,"name":"Christel Van den Eynde","orcid":null,"position":5,"is_corresponding":false},{"id":95311,"name":"Emilie Battivelli","orcid":"0000-0003-3297-0231","position":6,"is_corresponding":false},{"id":474885,"name":"Jerel Vega","orcid":"0000-0003-0998-4715","position":7,"is_corresponding":false},{"id":1077830,"name":"Éric Florence","orcid":"0000-0002-7004-9120","position":8,"is_corresponding":false},{"id":613839,"name":"Brigitte Autran","orcid":"0000-0002-3993-9336","position":9,"is_corresponding":false},{"id":233494,"name":"Nancie M. Archin","orcid":"0000-0002-7938-0389","position":10,"is_corresponding":false},{"id":105520,"name":"David M. Margolis","orcid":"0000-0001-5714-0002","position":11,"is_corresponding":false},{"id":1077831,"name":"Christine Katlama","orcid":"0000-0002-5093-4800","position":12,"is_corresponding":false},{"id":1078330,"name":"Chiraz Hamimi","orcid":null,"position":13,"is_corresponding":false},{"id":1078331,"name":"Ilse Van den Wyngaert","orcid":null,"position":14,"is_corresponding":false},{"id":1078332,"name":"Filmon Eyassu","orcid":null,"position":15,"is_corresponding":false},{"id":408658,"name":"Linos Vandekerckhove","orcid":"0000-0002-8600-1631","position":16,"is_corresponding":false},{"id":1078333,"name":"Daniel Boden","orcid":null,"position":17,"is_corresponding":false},{"id":1077828,"name":"Ellen Van Gulck","orcid":"0009-0008-3343-0644","position":0,"is_corresponding":true}],"reference_count":100,"raw_metadata":null,"created_at":"2026-07-19T01:11:08.077724Z","pmid":"37874295","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}