{"doi":"10.1128/9781555817381.ch134","title":"Reagents, Stains, and Media: Parasitology","abstract":null,"journal":"<i>Manual of</i>Clinical Microbiology","year":2015,"id":640206,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":899908,"name":"Susan E. Sharp","orcid":"0000-0002-0477-8624","position":1,"is_corresponding":false},{"id":1663813,"name":"Andrea J. Linscott","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Reagents, Stains, and Media: Parasitology","abstract":"The evaluation of clinical specimens for ova and parasites in the clinical laboratory can involve the use of direct macroscopic examination of the specimen and microscopic examination of fresh and preserved specimens, as well as culture for some parasitic organisms. These examinations necessitate the use of a variety of stains, reagents, and culture media, the most common of which are discussed in this chapter. Formalin has always been used in parasitology as an allpurpose preservative and in concentration procedures. The accompanying compound with the polyvinyl alcohol (PVA), specifically, mercuric chloride, zinc sulfate, or cupric sulfate, acts as the preservative and allows fixation of protozoan cysts and trophozoites for use with trichrome or iron hematoxylin stains for permanent smears. Specimens treated with Zn-PVA may also be stained with trichrome or iron hematoxylin stains for permanent smears. Specimens treated with Cu-PVA may also be stained with trichrome or iron hematoxylin stains for permanent smears. Examination of blood films for parasites includes the use of two common stains, the Giemsa stain and Wright's stain, both derivatives of the original Romanowsky stain. These stains are very similar, differing primarily in that no fixative is included in the Giemsa stain and the blood film must be fixed with absolute methanol prior to staining. In a basic staining procedure, thick films must be laked in distilled water or treated with saponin prior to performance of the staining procedure.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19910364","pmcid":null,"openalex_id":"https://openalex.org/W1530600706","authors":[],"funders":[],"total_grants":0,"fwci":1.9257,"citation_percentile":0.86956522,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":1},{"year":2016,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1128%2F9781555817381.ch134","host_type":"publisher"},{"url":"http://onlinelibrary.wiley.com/wol1/doi/10.1128/9781555817381.ch134/fullpdf","host_type":"publisher"},{"url":"https://doi.org/10.1128/9781555817381.ch134","host_type":"ebook platform"}],"fields_of_study":["Parasitic Infections and Diagnostics","Amoebic Infections and Treatments","Helminth infection and control"],"mesh_terms":[],"keywords":["Parasitology","Biology","Zoology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T07:55:52.822358Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}