{"doi":"10.1126/scitranslmed.abd9696","title":"Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR","abstract":"Ebola virus (EBOV) hemorrhagic fever outbreaks have been challenging to deter due to the lack of health care infrastructure in disease-endemic countries and a corresponding inability to diagnose and contain the disease at an early stage. EBOV vaccines and therapies have improved disease outcomes, but the advent of an affordable, easily accessed, mass-produced rapid diagnostic test (RDT) that matches the performance of more resource-intensive polymerase chain reaction (PCR) assays would be invaluable in containing future outbreaks. Here, we developed and demonstrated the performance of a new ultrasensitive point-of-care immunoassay, the EBOV D4 assay, which targets the secreted glycoprotein of EBOV. The EBOV D4 assay is 1000-fold more sensitive than the U.S. Food and Drug Administration-approved RDTs and detected EBOV infection earlier than PCR in a standard nonhuman primate model. The EBOV D4 assay is suitable for low-resource settings and may facilitate earlier detection, containment, and treatment during outbreaks of the disease.","journal":"Science Translational Medicine","year":2021,"id":159598,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":40,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9569,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":670814,"name":"Barbara D. Lipes","orcid":"0000-0003-1195-9463","position":1,"is_corresponding":false},{"id":572256,"name":"Jason Liu","orcid":"0000-0003-2287-2320","position":2,"is_corresponding":false},{"id":242845,"name":"Krystle N. Agans","orcid":"0000-0002-7319-6935","position":3,"is_corresponding":false},{"id":670815,"name":"Aiwei Yan","orcid":"0000-0001-6324-6786","position":4,"is_corresponding":false},{"id":670816,"name":"Patricia A. Shi","orcid":"0000-0002-7954-0055","position":5,"is_corresponding":false},{"id":670817,"name":"Daniela Cruz","orcid":"0000-0001-5701-1868","position":6,"is_corresponding":false},{"id":313942,"name":"Garrett Kelly","orcid":"0000-0001-9201-1302","position":7,"is_corresponding":false},{"id":670818,"name":"Kelli M. Luginbuhl","orcid":"0000-0002-6713-0331","position":8,"is_corresponding":false},{"id":360544,"name":"Daniel Y. Joh","orcid":"0000-0003-1301-1836","position":9,"is_corresponding":false},{"id":242848,"name":"Stephanie L. Foster","orcid":"0000-0002-3097-1129","position":10,"is_corresponding":false},{"id":572253,"name":"Jacob T. Heggestad","orcid":"0000-0001-5217-6735","position":11,"is_corresponding":false},{"id":573034,"name":"Angus Hucknall","orcid":null,"position":12,"is_corresponding":false},{"id":670819,"name":"Maiken H. Mikkelsen","orcid":"0000-0002-0487-7585","position":13,"is_corresponding":false},{"id":282555,"name":"Carl F. Pieper","orcid":"0000-0003-4809-1725","position":14,"is_corresponding":false},{"id":257433,"name":"Roarke Horstmeyer","orcid":"0000-0002-2480-9141","position":15,"is_corresponding":false},{"id":242852,"name":"Thomas W. Geisbert","orcid":"0000-0003-0858-1877","position":16,"is_corresponding":false},{"id":611093,"name":"Michael D. Gunn","orcid":"0000-0003-4602-0667","position":17,"is_corresponding":false},{"id":231603,"name":"Ashutosh Chilkoti","orcid":"0000-0002-1569-2228","position":18,"is_corresponding":false},{"id":572258,"name":"Cassio M. Fontes","orcid":"0000-0003-3157-478X","position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":null,"created_at":"2026-07-18T23:44:39.550981Z","pmid":"33827978","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}