{"doi":"10.1126/scitranslmed.aay1318","title":"Exosomes secreted by hiPSC-derived cardiac cells improve recovery from myocardial infarction in swine","abstract":"Cell therapy treatment of myocardial infarction (MI) is mediated, in part, by exosomes secreted from transplanted cells. Thus, we compared the efficacy of treatment with a mixture of cardiomyocytes (CMs; 10 million), endothelial cells (ECs; 5 million), and smooth muscle cells (SMCs; 5 million) derived from human induced pluripotent stem cells (hiPSCs), or with exosomes extracted from the three cell types, in pigs after MI. Female pigs received sham surgery; infarction without treatment (MI group); or infarction and treatment with hiPSC-CMs, hiPSC-ECs, and hiPSC-SMCs (MI + Cell group); with homogenized fragments from the same dose of cells administered to the MI + Cell group (MI + Fra group); or with exosomes (7.5 mg) extracted from a 2:1:1 mixture of hiPSC-CMs:hiPSC-ECs:hiPSC-SMCs (MI + Exo group). Cells and exosomes were injected into the injured myocardium. In vitro, exosomes promoted EC tube formation and microvessel sprouting from mouse aortic rings and protected hiPSC-CMs by reducing apoptosis, maintaining intracellular calcium homeostasis, and increasing adenosine 5'-triphosphate. In vivo, measurements of left ventricular ejection fraction, wall stress, myocardial bioenergetics, cardiac hypertrophy, scar size, cell apoptosis, and angiogenesis in the infarcted region were better in the MI + Cell, MI + Fra, and MI + Exo groups than in the MI group 4 weeks after infarction. The frequencies of arrhythmic events in animals from the MI, MI + Cell, and MI + Exo groups were similar. Thus, exosomes secreted by hiPSC-derived cardiac cells improved myocardial recovery without increasing the frequency of arrhythmogenic complications and may provide an acellular therapeutic option for myocardial injury.","journal":"Science Translational Medicine","year":2020,"id":50256,"datarank":0.7977179990766325,"base_score":5.318119993844216,"endowment":5.318119993844216,"self_citation_contribution":0.7977179990766325,"citation_network_contribution":0.0,"self_endowment_contribution":0.7977179990766325,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":203,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.949,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":246342,"name":"Lu Wang","orcid":"0000-0002-3550-4757","position":1,"is_corresponding":false},{"id":246343,"name":"Yuhua Wei","orcid":"0000-0003-0140-873X","position":2,"is_corresponding":false},{"id":246344,"name":"Prasanna Krishnamurthy","orcid":"0000-0002-4842-6364","position":3,"is_corresponding":false},{"id":246345,"name":"Gregory P. Walcott","orcid":"0000-0002-9807-7422","position":4,"is_corresponding":false},{"id":57434,"name":"Philippe Menasché","orcid":"0000-0002-9845-4064","position":5,"is_corresponding":false},{"id":246346,"name":"Jianyi Zhang","orcid":"0000-0002-3955-6554","position":6,"is_corresponding":false},{"id":246341,"name":"Ling Gao","orcid":"0000-0002-6391-6724","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T20:38:25.860949Z","pmid":"32938792","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}