{"doi":"10.1126/scisignal.abq5096","title":"B cell responses to membrane-presented antigens require the function of the mechanosensitive cation channel Piezo1","abstract":"The demand for a vaccine for coronavirus disease 2019 (COVID-19) highlighted gaps in our understanding of the requirements for B cell responses to antigens, particularly to membrane-presented antigens, as occurs in vivo. We found that human B cell responses to membrane-presented antigens required the function of Piezo1, a plasma membrane mechanosensitive cation channel. Simply making contact with a glass probe induced calcium (Ca 2+ ) fluxes in B cells that were blocked by the Piezo1 inhibitor GsMTx4. When placed on glass surfaces, the plasma membrane tension of B cells increased, which stimulated Ca 2+ influx and spreading of B cells over the glass surface, which was blocked by the Piezo1 inhibitor OB-1. B cell responses to membrane-presented antigens but not to soluble antigens were inhibited both by Piezo1 inhibitors and by siRNA-mediated knockdown of Piezo1. Thus, the activation of Piezo1 defines an essential event in B cell activation to membrane-presented antigens that may be exploited to improve the efficacy of vaccines.","journal":"Science Signaling","year":2023,"id":323125,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":864652,"name":"Haewon Sohn","orcid":"0000-0002-5126-0676","position":1,"is_corresponding":false},{"id":1037771,"name":"Rachel George","orcid":"0000-0001-8473-0358","position":2,"is_corresponding":false},{"id":1037772,"name":"Charles Torgbor","orcid":"0000-0002-7522-8216","position":3,"is_corresponding":false},{"id":829906,"name":"Javier Manzella‐Lapeira","orcid":null,"position":4,"is_corresponding":false},{"id":687597,"name":"Joseph Brzostowski","orcid":"0000-0003-0257-3905","position":5,"is_corresponding":false},{"id":333595,"name":"Susan K. Pierce","orcid":"0000-0001-7261-3437","position":6,"is_corresponding":false},{"id":1037770,"name":"Kihyuck Kwak","orcid":"0000-0002-7386-9589","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T01:07:52.377944Z","pmid":"37751477","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}