{"doi":"10.1126/sciimmunol.aeb2657","title":"Siglec-F <sup>+</sup> neutrophils promote the resolution of acute lung injury through ALOX15 induction","abstract":"Neutrophils have vital proinflammatory protective functions, but gene expression changes in neutrophils found in inflamed tissues suggest additional proresolving effects. We identified a neutrophil subset with a distinct phenotype and function that emerges in mouse lungs during resolution of injury. These resolution-phase neutrophils increased expression of Siglec-F (sialic acid–binding Ig-like lectin F), Alox15 (12/15-lipoxygenase), and Csf1 ( colony-stimulating factor 1 ). Siglec-F + neutrophils promoted macrophage differentiation and produced specialized proresolving mediators that accelerated injury resolution. Neutrophil depletion hindered lung epithelial catabatic responses, whereas adoptive transfer of Siglec-F + neutrophils accelerated restitution of lung epithelial cells. Transforming growth factor–β (TGF-β) and granulocyte-macrophage colony-stimulating factor (GM-CSF), acting via activator protein-1 (AP-1)/Jun, promoted expression of Siglec-F in mouse neutrophils and ALOX15 in mouse and human neutrophils. In patients with respiratory failure, ALOX15 + neutrophils were present in the bronchoalveolar lavage samples, and their frequency correlated with improved oxygenation. Thus, Siglec-F + ALOX15 + proresolving neutrophils contribute to tissue injury responses.","journal":"Science Immunology","year":2025,"id":525070,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.961,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":687796,"name":"Thayse R. Brüggemann","orcid":"0000-0003-3961-618X","position":1,"is_corresponding":false},{"id":640941,"name":"R. Cagnina","orcid":"0000-0002-4210-7928","position":2,"is_corresponding":false},{"id":648497,"name":"Robert Nshimiyimana","orcid":"0000-0003-0832-9938","position":3,"is_corresponding":false},{"id":1021026,"name":"Ana B. Villaseñor-Altamirano","orcid":"0000-0002-3940-5617","position":4,"is_corresponding":false},{"id":778307,"name":"Rafael M. Rezende","orcid":"0000-0003-3998-8012","position":5,"is_corresponding":false},{"id":553436,"name":"Toby B. Lanser","orcid":"0000-0002-2250-6075","position":6,"is_corresponding":false},{"id":368957,"name":"Xiaoli Liu","orcid":"0000-0003-2904-4413","position":7,"is_corresponding":false},{"id":246968,"name":"Marjorie Bateman","orcid":"0000-0003-3323-2337","position":8,"is_corresponding":false},{"id":687795,"name":"Nandini Krishnamoorthy","orcid":"0000-0002-1114-7118","position":9,"is_corresponding":false},{"id":722863,"name":"Stéphanie Pons","orcid":"0000-0002-7372-3563","position":10,"is_corresponding":false},{"id":706673,"name":"Melody G. Duvall","orcid":"0000-0002-4173-3628","position":11,"is_corresponding":false},{"id":4667,"name":"Raja-Elie E. Abdulnour","orcid":"0000-0002-8053-0145","position":12,"is_corresponding":false},{"id":642524,"name":"Alexander H. Tavares","orcid":"0000-0002-8363-4505","position":13,"is_corresponding":false},{"id":876292,"name":"Kathleen J. Haley","orcid":"0000-0002-3591-2291","position":14,"is_corresponding":false},{"id":1017164,"name":"Rajesh K. Krishnan","orcid":null,"position":15,"is_corresponding":false},{"id":242184,"name":"Charles N. Serhan","orcid":"0000-0003-4627-8545","position":16,"is_corresponding":false},{"id":40860,"name":"Bruce D. Levy","orcid":"0000-0001-9515-5731","position":17,"is_corresponding":false},{"id":1031925,"name":"Luciana P. Tavares","orcid":"0000-0002-5319-0210","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T02:50:16.562292Z","pmid":"41417925","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}